IB-CAS  > 中科院北方资源植物重点实验室
Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum
Li, Boqiang; Chen, Yong1; Zong, Yuanyuan; Shang, Yanjiao; Zhang, Zhanquan; Xu, Xiaodi1; Wang, Xiao1; Long, Manyuan2; Tian, Shiping1,3
2019
Source PublicationENVIRONMENTAL MICROBIOLOGY
ISSN1462-2912
Volume21Issue:3Pages:1124-1139
AbstractThe patulin biosynthesis is one of model pathways in an understanding of secondary metabolite biology and network novelties in fungi. However, molecular regulation mechanism of patulin biosynthesis and contribution of each gene related to the different catalytic enzymes in the biochemical steps of the pathway remain largely unknown in fungi. In this study, the genetic components of patulin biosynthetic pathway were systematically dissected in Penicillium expansum, which is an important fungal pathogen and patulin producer in harvested fruits and vegetables. Our results revealed that all the 15 genes in the cluster are involved in patulin biosynthesis. Proteins encoded by those genes are compartmentalized in various subcellular locations, including cytosol, nucleus, vacuole, endoplasmic reticulum, plasma membrane and cell wall. The subcellular localizations of some proteins, such as PatE and PatH, are required for the patulin production. Further, the functions of eight enzymes in the 10-step patulin biosynthetic pathway were verified in P. expansum. Moreover, velvet family proteins, VeA, VelB and VelC, were proved to be involved in the regulation of patulin biosynthesis, but not VosA. These findings provide a thorough understanding of the biosynthesis pathway, spatial control and regulation mechanism of patulin in fungi.
Subject AreaMicrobiology
DOI10.1111/1462-2920.14542
Indexed BySCI
Language英语
WOS KeywordSECONDARY METABOLISM ; ASPERGILLUS-NIDULANS ; EXPRESSION ; PATHWAY ; VIRULENCE ; COMPLEX ; PROTEIN ; PATHOGENICITY ; COORDINATION ; MYCOTOXIN
WOS Research AreaMicrobiology
WOS IDWOS:000461231300018
PublisherWILEY
SubtypeArticle
Publication PlaceHOBOKEN
EISSN1462-2920
Funding OrganizationNational Key R&D Program of China from Ministry of Science and Technology of the People's Republic of China [2016YFD0400902] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31722043] ; Youth Innovation Promotion Association CAS [2015063]
Corresponding Author Emailtsp@ibcas.ac.cn
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19398
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA
4.Minist Educ, Key Lab Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China
Recommended Citation
GB/T 7714
Li, Boqiang,Chen, Yong,Zong, Yuanyuan,et al. Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum[J]. ENVIRONMENTAL MICROBIOLOGY,2019,21(3):1124-1139.
APA Li, Boqiang.,Chen, Yong.,Zong, Yuanyuan.,Shang, Yanjiao.,Zhang, Zhanquan.,...&Tian, Shiping.(2019).Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum.ENVIRONMENTAL MICROBIOLOGY,21(3),1124-1139.
MLA Li, Boqiang,et al."Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum".ENVIRONMENTAL MICROBIOLOGY 21.3(2019):1124-1139.
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