Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis

doi:10.1016/j.envexpbot.2019.02.002

	Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis
	Hou, Longyu ; Wang, Mingya 3; Wang, Hui 4; Zhang, Wen-Hao; Mao, Peisheng
	2019
发表期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN	0098-8472
卷号	162 页码:95-102
摘要	Seed dormancy is an important trait determining seed germination and seedling establishment of plants. Despite extensive studies on seed dormancy, relatively few studies have specifically focused on mechanisms underlying seed dormancy in wild perennial species. Leymus chinensis is a perennial grass forage with excellent nutritional value and one of the dominant species in the Eurasian steppe. In this study, the proteomic features for seed dormancy and release in the wild germplasm after ripening were analyzed by iTRAQ technology. Based on the wheat (Triticwn aestivum) protein database, a total of 281 differently expressed proteins were identified in L. chinensis non-dormant seeds versus dormant seeds, with 188 differentially decreased (fold change < 0.8, P < 0.05) and 93 differentially increased proteins (fold change > 1.2, P < 0.05). Based on the COG annotation, the proteins involved in chromatin structure and dynamics, intracellular trafficking, section, and vesicular transport, cytoskeleton were all increased differentially. In contrast, the proteins involved in RNA processing and modification, cell wall/membrane/envelope biogenesis, and signal transduction mechanisms were all decreased differentially. Based on the Mapman annotation and function enrichment, the decreased proteins mainly participated in photosynthesis and TCA cycle, while the increased proteins were involved in protein targeting, degradation, and synthesis, amino acid metabolism, lipid metabolism, redox, cell cycle, and DNA synthesis/ chromatin structure. After-ripening of L. chinensis dormant seeds was the transition from maturation to storage. Our proteomic analyses revealed that the dormancy release of L. chinensis seeds was related to the increases in tubulin, histone, and thioredoxins, and the decrease in L- ascorbate peroxidase. Based on these results, we proposed that an increase in reactive oxygen species (ROS) during after-ripening is an important driver to regulate dormancy release in L. chinensis seeds by modulating cytoskeleton and chromatin. These findings provide new insight for our mechanistic understanding of dormancy control in perennial grass forage.
关键词	Proteomics Seed dormancy and germination After-ripening Leymus chinensis Reactive oxygen species
学科领域	Plant Sciences ; Environmental Sciences
DOI	10.1016/j.envexpbot.2019.02.002
收录类别	SCI
语种	英语
WOS关键词	FLOW CYTOMETRIC DETERMINATION ; NUCLEAR REPLICATION STAGES ; BETA-TUBULIN ACCUMULATION ; GENE-EXPRESSION ; TRIN. TZVEL. ; GERMINATION ; ARABIDOPSIS ; TEMPERATURE ; MECHANISM ; MATURATION
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
WOS记录号	WOS:000467507700010
出版者	PERGAMON-ELSEVIER SCIENCE LTD
文献子类	Article
出版地	OXFORD
EISSN	1873-7307
资助机构	earmarked fund for the China Agriculture Research System [CARS-34] ; National Key Technologies R&D Program of the 12th Five-Year Plan [2011BAD17B01-02] ; Beijing Common Construction Project
作者邮箱	maops@cau.edu.cn
作品OA属性	hybrid
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19418
专题	植被与环境变化国家重点实验室
作者单位	1.China Agr Univ, Forage Seed Lab, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inner Mongolia Res Ctr Prataculture, Beijing 100093, Peoples R China 4.Hebei Agr Univ, Coll Anim Sci & Technol, Baoding 071000, Peoples R China 5.Southwest Minzu Univ, Coll Qinghai Tibetan Plateau, Chengdu 610041, Peoples R China
推荐引用方式 GB/T 7714	Hou, Longyu,Wang, Mingya,Wang, Hui,et al. Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2019,162:95-102.
APA	Hou, Longyu,Wang, Mingya,Wang, Hui,Zhang, Wen-Hao,&Mao, Peisheng.(2019).Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,162,95-102.
MLA	Hou, Longyu,et al."Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 162(2019):95-102.

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