IB-CAS  > 植被与环境变化国家重点实验室
Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis
Hou, Longyu; Wang, Mingya3; Wang, Hui4; Zhang, Wen-Hao; Mao, Peisheng
2019
Source PublicationENVIRONMENTAL AND EXPERIMENTAL BOTANY
ISSN0098-8472
Volume162Pages:95-102
AbstractSeed dormancy is an important trait determining seed germination and seedling establishment of plants. Despite extensive studies on seed dormancy, relatively few studies have specifically focused on mechanisms underlying seed dormancy in wild perennial species. Leymus chinensis is a perennial grass forage with excellent nutritional value and one of the dominant species in the Eurasian steppe. In this study, the proteomic features for seed dormancy and release in the wild germplasm after ripening were analyzed by iTRAQ technology. Based on the wheat (Triticwn aestivum) protein database, a total of 281 differently expressed proteins were identified in L. chinensis non-dormant seeds versus dormant seeds, with 188 differentially decreased (fold change < 0.8, P < 0.05) and 93 differentially increased proteins (fold change > 1.2, P < 0.05). Based on the COG annotation, the proteins involved in chromatin structure and dynamics, intracellular trafficking, section, and vesicular transport, cytoskeleton were all increased differentially. In contrast, the proteins involved in RNA processing and modification, cell wall/membrane/envelope biogenesis, and signal transduction mechanisms were all decreased differentially. Based on the Mapman annotation and function enrichment, the decreased proteins mainly participated in photosynthesis and TCA cycle, while the increased proteins were involved in protein targeting, degradation, and synthesis, amino acid metabolism, lipid metabolism, redox, cell cycle, and DNA synthesis/ chromatin structure. After-ripening of L. chinensis dormant seeds was the transition from maturation to storage. Our proteomic analyses revealed that the dormancy release of L. chinensis seeds was related to the increases in tubulin, histone, and thioredoxins, and the decrease in L- ascorbate peroxidase. Based on these results, we proposed that an increase in reactive oxygen species (ROS) during after-ripening is an important driver to regulate dormancy release in L. chinensis seeds by modulating cytoskeleton and chromatin. These findings provide new insight for our mechanistic understanding of dormancy control in perennial grass forage.
KeywordProteomics Seed dormancy and germination After-ripening Leymus chinensis Reactive oxygen species
Subject AreaPlant Sciences ; Environmental Sciences
DOI10.1016/j.envexpbot.2019.02.002
Indexed BySCI
Language英语
WOS KeywordFLOW CYTOMETRIC DETERMINATION ; NUCLEAR REPLICATION STAGES ; BETA-TUBULIN ACCUMULATION ; GENE-EXPRESSION ; TRIN. TZVEL. ; GERMINATION ; ARABIDOPSIS ; TEMPERATURE ; MECHANISM ; MATURATION
WOS Research AreaPlant Sciences ; Environmental Sciences & Ecology
WOS IDWOS:000467507700010
PublisherPERGAMON-ELSEVIER SCIENCE LTD
SubtypeArticle
Publication PlaceOXFORD
EISSN1873-7307
Funding Organizationearmarked fund for the China Agriculture Research System [CARS-34] ; National Key Technologies R&D Program of the 12th Five-Year Plan [2011BAD17B01-02] ; Beijing Common Construction Project
Corresponding Author Emailmaops@cau.edu.cn
OAhybrid
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19418
Collection植被与环境变化国家重点实验室
Affiliation1.China Agr Univ, Forage Seed Lab, Beijing 100193, Peoples R China
2.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
3.Chinese Acad Sci, Inner Mongolia Res Ctr Prataculture, Beijing 100093, Peoples R China
4.Hebei Agr Univ, Coll Anim Sci & Technol, Baoding 071000, Peoples R China
5.Southwest Minzu Univ, Coll Qinghai Tibetan Plateau, Chengdu 610041, Peoples R China
Recommended Citation
GB/T 7714
Hou, Longyu,Wang, Mingya,Wang, Hui,et al. Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2019,162:95-102.
APA Hou, Longyu,Wang, Mingya,Wang, Hui,Zhang, Wen-Hao,&Mao, Peisheng.(2019).Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,162,95-102.
MLA Hou, Longyu,et al."Physiological and proteomic analyses for seed dormancy and release in the perennial grass of Leymus chinensis".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 162(2019):95-102.
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