Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics

doi:10.1016/j.envint.2019.05.013

IB-CAS > 中科院光生物学重点实验室

	Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics
	Li, Huahua ; Mei, Xiaoxue ; Liu, Bingfeng ; Li, Zhen ; Wang, Baichen 1; Ren, Nanqi ; Xing, Defeng
	2019
发表期刊	ENVIRONMENT INTERNATIONAL
ISSN	0160-4120
卷号	129 页码:1-9
摘要	Ethanoligenens, a novel ethanologenic hydrogen-producing genus, is a representative fermenter in its unique acetate-ethanol fermentation and physiology. Acetic acid accumulation is one of major factors that affect H-2-ethanol co-production. However, sufficient information is unavailable on the tolerance mechanisms of hydrogen-producing bacterium in acetic acid stress. The fermentation process of Ethanoligenens harbinense YUAN-3 was significantly slowed down in the selection stress of exogenous acetic acid. The maximum gas production rate of strain YUAN-3 decreased from 192.15 mL.(L-culture)(-1).h(-1) to 75.2 mL.(L-culture)(-1).h(-1) with increasing exogenous acetic acid from 0 mM to 30 mM, the batch fermentation period was correspondingly expanded from 66 h to 136 h. Through iTRAQ-based quantitative proteomic approach, 78, 121 and 216 proteins were differentially expressed after strain YUAN-3 was cultured in the medium supplemented with exogenous acetic acid of 10 mM, 20 mM and 30 mM. The up-regulated proteins were mainly involved in beta-alanine and pyrimidine metabolism, oxidative stress response, while the down-regulated proteins mainly participated in phosphotransferase system (PTS), fructose and mannose metabolism, phosphate uptake, ribosome, and flagellar assembly. These proteins help to maintain balance between fermentation process and alleviation of intracellular acidification in strain YUAN-3. The study indicated that response to acetic acid stress in strain YUAN-3 was a complex process, which involved multiple metabolic pathways. Reductive pyrimidine catabolic pathway played an important role in the acetic acid resistance of E. harbinense.
关键词	Hydrogen-producing bacteria Ethanoligenens harbinense Acetate-ethanol fermentation Acetic acid tolerance Quantitative proteomics iTRAQ
学科领域	Environmental Sciences
DOI	10.1016/j.envint.2019.05.013
收录类别	SCI
语种	英语
WOS关键词	BIOHYDROGEN PRODUCTION ; PROTEIN EXPRESSION ; BACTERIA ; REVEALS ; PH ; MECHANISMS ; TOLERANCE ; ENZYMES
WOS研究方向	Environmental Sciences & Ecology
WOS记录号	WOS:000470239200001
出版者	PERGAMON-ELSEVIER SCIENCE LTD
文献子类	Article
出版地	OXFORD
EISSN	1873-6750
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31870114] ; National Key Research and Development Program of China [2018YFD1100503] ; State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology)Harbin Institute of Technology [HCK201018]
作者邮箱	dxing@hit.edu.cn
作品OA属性	gold
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19424
专题	中科院光生物学重点实验室
作者单位	1.Harbin Inst Technol, Sch Environm, State Key Lab Urban Water Resource & Environm, Harbin 150090, Heilongjiang, Peoples R China 2.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Huahua,Mei, Xiaoxue,Liu, Bingfeng,et al. Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics[J]. ENVIRONMENT INTERNATIONAL,2019,129:1-9.
APA	Li, Huahua.,Mei, Xiaoxue.,Liu, Bingfeng.,Li, Zhen.,Wang, Baichen.,...&Xing, Defeng.(2019).Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics.ENVIRONMENT INTERNATIONAL,129,1-9.
MLA	Li, Huahua,et al."Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics".ENVIRONMENT INTERNATIONAL 129(2019):1-9.

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