Cloning and expression analysis of LoCCD8 during IAA-induced bulbils outgrowth in lily (Oriental Hybrid 'Sorbonne')

doi:10.1016/j.jplph.2019.03.002

	Cloning and expression analysis of LoCCD8 during IAA-induced bulbils outgrowth in lily (Oriental Hybrid 'Sorbonne')
	Li, Jingrui 1,2; Chai, Min 1; Zhu, Xiaopei 1; Zhang, Xuhong 1; Li, Hui 1; Wang, Di 1; Xing, Quan 1; Zhang, Jinzheng 1; Sun, Meiyu 1; Shi, Lei1
	2019
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	236 页码:39-50
摘要	Aerial bulbils, which resemble tiny bulbs, develop from axillary buds and facilitate rapid propagation of Lilium. In most species of lily, bulbils are perpetually dormant and little is known about induction of these vegetative structures. Herein, we proposed that strigolactones (SLs) may regulate the induction of bulbils in Lilium. We tested this hypothesis by isolating and investigating the expression patterns of 2 copies of the carotenoid cleavage dioxygenase8 (CCD8) gene in lily-LoCCD8a and LoCCD8b-with regard to biosynthesis of SLs. Expression analyses revealed that LoCCD8a principally is expressed during vegetative growth, whereas LoCCD8b mainly is expressed during reproductive growth. The maximum quantity of LoCCD8a transcripts was observed in the basal plate in most developmental stages, which suggests that SLs may originate from underground parts, especially the basal plate, and move upward. The effects of treatments with indole-3-acetic acid or SL analog (GR24) on outgrowth of bulbils and expression of LoCCD8 genes suggested that SLs function downstream of auxin to inhibit the outgrowth of bulbil. The expression patterns of LoCCD8a and LoCCD8b at sprouting and bulblet weighting stages also implied that SLs may function in nutrient redistribution. Our findings are expected to promote the utilization of bulbils as vegetative propagules for commercial practice.
关键词	Lily Auxin SLs CCD8 Bulbil
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2019.03.002
收录类别	SCI
语种	英语
WOS关键词	BUD OUTGROWTH ; LEAF SENESCENCE ; AUXIN ; STRIGOLACTONE ; PLANT ; SIGNALS ; GROWTH ; PEA ; REPRODUCTION ; GERMINATION
WOS研究方向	Plant Sciences
WOS记录号	WOS:000466148400006
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	Chinese Academy of SciencesChinese Academy of Sciences [KFZD-SW-313] ; National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2011AA10020805]
作者邮箱	sunmeiyu@ibcas.ac.cn ; shilei_67@126.com
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19428
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Jingrui,Chai, Min,Zhu, Xiaopei,et al. Cloning and expression analysis of LoCCD8 during IAA-induced bulbils outgrowth in lily (Oriental Hybrid 'Sorbonne')[J]. JOURNAL OF PLANT PHYSIOLOGY,2019,236:39-50.
APA	Li, Jingrui.,Chai, Min.,Zhu, Xiaopei.,Zhang, Xuhong.,Li, Hui.,...&Shi, Lei.(2019).Cloning and expression analysis of LoCCD8 during IAA-induced bulbils outgrowth in lily (Oriental Hybrid 'Sorbonne').JOURNAL OF PLANT PHYSIOLOGY,236,39-50.
MLA	Li, Jingrui,et al."Cloning and expression analysis of LoCCD8 during IAA-induced bulbils outgrowth in lily (Oriental Hybrid 'Sorbonne')".JOURNAL OF PLANT PHYSIOLOGY 236(2019):39-50.

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