IB-CAS  > 中科院北方资源植物重点实验室
Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis
Sun, Xiaoming1; Zhu, Zhenfei3; Zhang, Langlang; Fang, Linchuan; Zhang, Jisen2; Wang, Qingfeng; Li, Shaohua1; Liang, Zhenchang1; Xin, Haiping
2019
发表期刊SCIENTIA HORTICULTURAE
ISSN0304-4238
卷号243页码:320-326
摘要Low temperature is a vital environmental factor that affects plant growth, development and crop yields. Ethylene response factor (ERF) is a plant specific transcription factor with important roles in stress adaptation. However, the regulatory mechanism on how grapevine responds to cold stress is still unclear. In this study, we isolated and characterized two ERF genes from Vitis amurensis designated as VaERF080 and VaERF087. The function of these two genes was investigated in transgenic Arabidopsis. We found that both VaERF080 and VaERF087 were induced by cold stress and ethylene. The overexpression of these genes in Arabidopsis resulted in enhanced cold tolerance. The VaERF080- and VaERF087-overexpressing (OE) Arabidopsis lines showed lower levels of malondialdehyde (MDA) content and higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) under cold stress compared to wild-type (WT) Arabidopsis. Nine representative cold-responsive genes, CBF1, CBF2, ICE1, ZAT12, KIN1, SIZ1, RD29A, COR15A, and COR47, were significantly upregulated in OE Arabidopsis. These results revealed that both VaERF080 and VaERF087 may improve the cold tolerance of transgenic Arabidopsis through increasing antioxidant enzyme activities and regulating the expression of cold-related genes.
关键词Abiotic stress Amur grape Antioxidant enzyme Cold stress Low temperature
学科领域Horticulture
DOI10.1016/j.scienta.2018.08.055
收录类别SCI
语种英语
WOS关键词ABIOTIC STRESS TOLERANCE ; REGULATES FREEZING TOLERANCE ; TRANSCRIPTION FACTOR ; SALT STRESS ; GENE ; CBF ; ACCLIMATION ; EXPRESSION ; FAMILY ; RICE
WOS研究方向Agriculture
WOS记录号WOS:000450381800039
出版者ELSEVIER
文献子类Article
出版地AMSTERDAM
EISSN1879-1018
资助机构Youth Innovation Promotion Association of CAS [2015281] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016M601166] ; Grape Breeding Project of Ningxia [NXNYYZ201502] ; Open Project Program of the Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology (Fujian Agriculture and Forestry University)
作者邮箱xinhaiping215@hotmail.com
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/19431
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
3.Fujian Agr & Forestry Univ, Ctr Genom & Biotechnol, Haixia Inst Sci & Technol, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Fujian, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Sun, Xiaoming,Zhu, Zhenfei,Zhang, Langlang,et al. Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis[J]. SCIENTIA HORTICULTURAE,2019,243:320-326.
APA Sun, Xiaoming.,Zhu, Zhenfei.,Zhang, Langlang.,Fang, Linchuan.,Zhang, Jisen.,...&Xin, Haiping.(2019).Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis.SCIENTIA HORTICULTURAE,243,320-326.
MLA Sun, Xiaoming,et al."Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis".SCIENTIA HORTICULTURAE 243(2019):320-326.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S030442381830(2137KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, Xiaoming]的文章
[Zhu, Zhenfei]的文章
[Zhang, Langlang]的文章
百度学术
百度学术中相似的文章
[Sun, Xiaoming]的文章
[Zhu, Zhenfei]的文章
[Zhang, Langlang]的文章
必应学术
必应学术中相似的文章
[Sun, Xiaoming]的文章
[Zhu, Zhenfei]的文章
[Zhang, Langlang]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S0304423818306071-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。