Knowledge Management System Of Institute Of Botany,CAS
Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis | |
Sun, Xiaoming1; Zhu, Zhenfei3; Zhang, Langlang; Fang, Linchuan; Zhang, Jisen2; Wang, Qingfeng; Li, Shaohua1; Liang, Zhenchang1; Xin, Haiping | |
2019 | |
发表期刊 | SCIENTIA HORTICULTURAE |
ISSN | 0304-4238 |
卷号 | 243页码:320-326 |
摘要 | Low temperature is a vital environmental factor that affects plant growth, development and crop yields. Ethylene response factor (ERF) is a plant specific transcription factor with important roles in stress adaptation. However, the regulatory mechanism on how grapevine responds to cold stress is still unclear. In this study, we isolated and characterized two ERF genes from Vitis amurensis designated as VaERF080 and VaERF087. The function of these two genes was investigated in transgenic Arabidopsis. We found that both VaERF080 and VaERF087 were induced by cold stress and ethylene. The overexpression of these genes in Arabidopsis resulted in enhanced cold tolerance. The VaERF080- and VaERF087-overexpressing (OE) Arabidopsis lines showed lower levels of malondialdehyde (MDA) content and higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) under cold stress compared to wild-type (WT) Arabidopsis. Nine representative cold-responsive genes, CBF1, CBF2, ICE1, ZAT12, KIN1, SIZ1, RD29A, COR15A, and COR47, were significantly upregulated in OE Arabidopsis. These results revealed that both VaERF080 and VaERF087 may improve the cold tolerance of transgenic Arabidopsis through increasing antioxidant enzyme activities and regulating the expression of cold-related genes. |
关键词 | Abiotic stress Amur grape Antioxidant enzyme Cold stress Low temperature |
学科领域 | Horticulture |
DOI | 10.1016/j.scienta.2018.08.055 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | ABIOTIC STRESS TOLERANCE ; REGULATES FREEZING TOLERANCE ; TRANSCRIPTION FACTOR ; SALT STRESS ; GENE ; CBF ; ACCLIMATION ; EXPRESSION ; FAMILY ; RICE |
WOS研究方向 | Agriculture |
WOS记录号 | WOS:000450381800039 |
出版者 | ELSEVIER |
文献子类 | Article |
出版地 | AMSTERDAM |
EISSN | 1879-1018 |
资助机构 | Youth Innovation Promotion Association of CAS [2015281] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016M601166] ; Grape Breeding Project of Ningxia [NXNYYZ201502] ; Open Project Program of the Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology (Fujian Agriculture and Forestry University) |
作者邮箱 | xinhaiping215@hotmail.com |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19431 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Fujian Agr & Forestry Univ, Ctr Genom & Biotechnol, Haixia Inst Sci & Technol, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Fujian, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Xiaoming,Zhu, Zhenfei,Zhang, Langlang,et al. Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis[J]. SCIENTIA HORTICULTURAE,2019,243:320-326. |
APA | Sun, Xiaoming.,Zhu, Zhenfei.,Zhang, Langlang.,Fang, Linchuan.,Zhang, Jisen.,...&Xin, Haiping.(2019).Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis.SCIENTIA HORTICULTURAE,243,320-326. |
MLA | Sun, Xiaoming,et al."Overexpression of ethylene response factors VaERF080 and VaERF087 from Vitis amurensis enhances cold tolerance in Arabidopsis".SCIENTIA HORTICULTURAE 243(2019):320-326. |
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