Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation

doi:10.1016/j.ijhydene.2019.10.074

IB-CAS > 中科院光生物学重点实验室

	Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation
	Chen, Mei ; Liu, Peng 1; Zhang, Fin 1; Peng, Lianwei 2; Huang, Fang
	2019
发表期刊	INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
ISSN	0360-3199
卷号	44 期号:60 页码:31790-31799
摘要	Renewable H-2 photoproduction by Chlamydomonas reinhardtii offers a desirable bio-system for solar fuels. However, its large-scale application is hindered mainly due to lack of ideal strains. We previously isolated a mutant hpm91 which lacks PGR5 and sustains H-2 photoproduction for 25 days. To understand the photosynthetic basis for this remarkable phenotype, we hereby investigated its photochemical characteristics during sulfur-deprived H-2 photoproduction using in vivo chlorophyll fluorescence spectroscopy. Compared to wild type, effective quantum yield of PSII and PSI of hpm91 increased upto 78.9% and 147.6%, respectively. Electron transport rate of each photosystem is closely correlated with the increase of quantum yield, suggesting overall enhanced photochemistry of hpm91 under such condition. Moreover, ATP synthase activity decays slower and remains higher in this mutant. These are in vivo evidence demonstrating increased photosynthetic efficiency of hpm91 promotes its H2 photoproduction. Together with its competent photoheterotrophic growth in a larger photobioreactor, we propose that hpm91 is a valuable strain for re-engineering Chlamydomonas towards improving light energy efficiency in a large-scale system. (C) 2019 Institute of Botany, Chinese Academy of Sciences. Published by Elsevier Ltd on behalf of Hydrogen Energy Publications LLC.
关键词	C. reinhardtii Hydrogen production mutant 91 Sulfur deprivation PSI PSII ATP synthase activity
学科领域	Chemistry, Physical ; Electrochemistry ; Energy & Fuels
DOI	10.1016/j.ijhydene.2019.10.074
收录类别	SCI
语种	英语
WOS关键词	CYCLIC ELECTRON FLOW ; HYDROGEN-PRODUCTION ; PHOTOSYSTEM-II ; CHLOROPHYLL FLUORESCENCE ; THYLAKOID MEMBRANE ; GREEN-ALGAE ; HIGH-LIGHT ; REINHARDTII ; PROTEIN ; TRANSPORT
WOS研究方向	Chemistry ; Electrochemistry ; Energy & Fuels
WOS记录号	WOS:000502888800009
出版者	PERGAMON-ELSEVIER SCIENCE LTD
文献子类	Article
出版地	OXFORD
EISSN	1879-3487
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470340] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015CB150100] ; CASChinese Academy of Sciences [17030300, KGCX2-YW-373]
作者邮箱	fhuang@ibcas.ac.cn
作品OA属性	hybrid
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19433
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China
推荐引用方式 GB/T 7714	Chen, Mei,Liu, Peng,Zhang, Fin,et al. Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2019,44(60):31790-31799.
APA	Chen, Mei,Liu, Peng,Zhang, Fin,Peng, Lianwei,&Huang, Fang.(2019).Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,44(60),31790-31799.
MLA	Chen, Mei,et al."Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 44.60(2019):31790-31799.

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