Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars

doi:10.1016/j.plaphy.2019.06.018

	Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars
	Deng, Chengyan 1; Li, Shanshan 2,3,4; Feng, Chengyong 2,3,4; Hong, Yan 1; Huang, He 1; Wang, Jiaying 1; Wang, Liangsheng2,3,4 ; Dai, Silan 1
	2019
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	142 页码:22-33
摘要	Centaurea cyanus is a popular garden plant native to Europe. Although their petals show abundant colour variations, the flavonoid profiling and the potential molecular mechanisms remain unclear. In the present study, we collected six cornflower cultivars with white, pink, red, blue, mauve and black petals. Ultra-performance liquid chromatography coupled with photodiode array and tandem mass spectrometry (UPLC-MS/MS) was used to investigate the comparative profiling of flavonoids both qualitatively and quantitatively. Ten anthocyanins, six flavones and two flavonols were separated and putatively identified. Except for white petals without any anthocyanins, both pink and red flowers contained pelargonidin derivatives, whereas blue, mauve and black petals accumulated cyanidins. The expression patterns of genes involved in the flavonoid biosynthesis were performed by real-time quantitative reverse transcription-PCR. The anthocyanin biosynthetic pathway in white petals was inhibited starting from flavanone 3-hydroxylase, resulting in the absence of anthocyanin accumulation. The open reading frame of flavonoid 3'-hydroxylase in pink and red petals was truncated; this led to loss of a haem binding site, a conserved motif in the cytochrome P450 family, and loss of conversion from dihydrokaempferol to dihydroquercetin. The significantly higher expression of structural genes corresponding to the hyper-accumulation of flavonoids in black petals may play an important role in black coloration. Remarkably, the mauve and blue petals accumulated the same cyanidin derivative but contained apigenin with different modifications on the 4' position, which may cause the coloration differences. The results obtained in this study will provide insights into the mechanisms of vivid colour diversities in cornflower.
关键词	Centaurea cyanus Petal colour Flavonoids Gene expression Biosynthetic pathway
学科领域	Plant Sciences
DOI	10.1016/j.plaphy.2019.06.018
收录类别	SCI
语种	英语
WOS关键词	3-(6-SUCCINYL GLUCOSIDE)-5-GLUCOSIDE ; BLUE COLORATION ; ANTHOCYANIN ; FLOWERS ; PIGMENT ; IDENTIFICATION ; IONS ; ACID
WOS研究方向	Plant Sciences
WOS记录号	WOS:000487569200004
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
文献子类	Article
出版地	ISSY-LES-MOULINEAUX
资助机构	National Key Research and Development Program [2018YFD1000405]
作者邮箱	silandai@sina.com
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19443
专题	中科院北方资源植物重点实验室
作者单位	1.Beijing Forestry Univ, Beijing Lab Urban & Rural Ecol Environm, Natl Engn Res Ctr Floriculture, Beijing Key Lab Ornamental Plants Germplasm Innov, Beijing 100083, Peoples R China 2.Beijing Forestry Univ, Coll Landscape Architecture, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Deng, Chengyan,Li, Shanshan,Feng, Chengyong,et al. Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2019,142:22-33.
APA	Deng, Chengyan.,Li, Shanshan.,Feng, Chengyong.,Hong, Yan.,Huang, He.,...&Dai, Silan.(2019).Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars.PLANT PHYSIOLOGY AND BIOCHEMISTRY,142,22-33.
MLA	Deng, Chengyan,et al."Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars".PLANT PHYSIOLOGY AND BIOCHEMISTRY 142(2019):22-33.

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