IB-CAS  > 中科院北方资源植物重点实验室
Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars
Deng, Chengyan1; Li, Shanshan2,3,4; Feng, Chengyong2,3,4; Hong, Yan1; Huang, He1; Wang, Jiaying1; Wang, Liangsheng2,3,4; Dai, Silan1
2019
Source PublicationPLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN0981-9428
Volume142Pages:22-33
AbstractCentaurea cyanus is a popular garden plant native to Europe. Although their petals show abundant colour variations, the flavonoid profiling and the potential molecular mechanisms remain unclear. In the present study, we collected six cornflower cultivars with white, pink, red, blue, mauve and black petals. Ultra-performance liquid chromatography coupled with photodiode array and tandem mass spectrometry (UPLC-MS/MS) was used to investigate the comparative profiling of flavonoids both qualitatively and quantitatively. Ten anthocyanins, six flavones and two flavonols were separated and putatively identified. Except for white petals without any anthocyanins, both pink and red flowers contained pelargonidin derivatives, whereas blue, mauve and black petals accumulated cyanidins. The expression patterns of genes involved in the flavonoid biosynthesis were performed by real-time quantitative reverse transcription-PCR. The anthocyanin biosynthetic pathway in white petals was inhibited starting from flavanone 3-hydroxylase, resulting in the absence of anthocyanin accumulation. The open reading frame of flavonoid 3'-hydroxylase in pink and red petals was truncated; this led to loss of a haem binding site, a conserved motif in the cytochrome P450 family, and loss of conversion from dihydrokaempferol to dihydroquercetin. The significantly higher expression of structural genes corresponding to the hyper-accumulation of flavonoids in black petals may play an important role in black coloration. Remarkably, the mauve and blue petals accumulated the same cyanidin derivative but contained apigenin with different modifications on the 4' position, which may cause the coloration differences. The results obtained in this study will provide insights into the mechanisms of vivid colour diversities in cornflower.
KeywordCentaurea cyanus Petal colour Flavonoids Gene expression Biosynthetic pathway
Subject AreaPlant Sciences
DOI10.1016/j.plaphy.2019.06.018
Indexed BySCI
Language英语
WOS Keyword3-(6-SUCCINYL GLUCOSIDE)-5-GLUCOSIDE ; BLUE COLORATION ; ANTHOCYANIN ; FLOWERS ; PIGMENT ; IDENTIFICATION ; IONS ; ACID
WOS Research AreaPlant Sciences
WOS IDWOS:000487569200004
PublisherELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
SubtypeArticle
Publication PlaceISSY-LES-MOULINEAUX
Funding OrganizationNational Key Research and Development Program [2018YFD1000405]
Corresponding Author Emailsilandai@sina.com
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19443
Collection中科院北方资源植物重点实验室
Affiliation1.Beijing Forestry Univ, Beijing Lab Urban & Rural Ecol Environm, Natl Engn Res Ctr Floriculture, Beijing Key Lab Ornamental Plants Germplasm Innov, Beijing 100083, Peoples R China
2.Beijing Forestry Univ, Coll Landscape Architecture, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
4.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Deng, Chengyan,Li, Shanshan,Feng, Chengyong,et al. Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2019,142:22-33.
APA Deng, Chengyan.,Li, Shanshan.,Feng, Chengyong.,Hong, Yan.,Huang, He.,...&Dai, Silan.(2019).Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars.PLANT PHYSIOLOGY AND BIOCHEMISTRY,142,22-33.
MLA Deng, Chengyan,et al."Metabolite and gene expression analysis reveal the molecular mechanism for petal colour variation in six Centaurea cyanus cultivars".PLANT PHYSIOLOGY AND BIOCHEMISTRY 142(2019):22-33.
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