SIZ1-Mediated SUMOylation of TPR1 Suppresses Plant Immunity in Arabidopsis

doi:10.1016/j.molp.2018.12.002

	SIZ1-Mediated SUMOylation of TPR1 Suppresses Plant Immunity in Arabidopsis
	Niu, De ; Lin, Xiao-Li ; Kong, Xiangxiong ; Qu, Gao-Ping ; Cai, Bin ; Lee, Jiyoung 2; Jin, Jing Bo
	2019
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	12 期号:2 页码:215-228
摘要	Plant immune responses are tightly regulated to ensure their appropriate deployment. Overexpression of TOPLESS-RELATED 1 (TPR1), a SUPPRESSOR OF npr1-1, CONSTITUTIVE 1 (SNC1)-interacting protein, results in autoimmunity that reduces plant growth and development. However, how TPR1 activity is regulated remains unknown. Loss of function of SIZ1, a (SUMO) E3 ligase, induces an autoimmune response, partially due to elevated SNC1 levels. Here we show that SNC1 expression is upregulated in Arabidopsis thaliana siz1-2 due to positive-feedback regulation by salicylic acid. SIZ1 physically interacts with TPR1 and facilitates its SUMO modification. The K282 and K721 residues in TPR1 serve as critical SUMO attachment sites. Simultaneous introduction of K282R and K721R substitutions in TPR1 blocked its SUMOylation, enhanced its transcriptional co-repressor activity, and increased its association with HISTONE DEACETYLASE 19 (HDA19), suggesting that SUMOylation of TPR1 represses its transcriptional co-repressor activity and inhibits its interaction with HDA19. In agreement with this finding, the simultaneous introduction of K282R and K721R substitutions enhanced TPR1-mediated immunity, and the tpr1 mutation partially suppressed autoimmunity in siz1-2. These results demonstrate that SIZ1-mediated SUMOylation of TPR1 represses plant immunity, which at least partly contributes to the suppression of autoimmunity under nonpathogenic conditions to ensure proper plant development.
关键词	immune response SIZ1 SUMO E3 ligase SUMO modification TOPLESS
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2018.12.002
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTORS INTERACT ; SALICYLIC-ACID ; LIGASE SIZ1 ; RESISTANCE PROTEINS ; DISEASE RESISTANCE ; SUMO CONJUGATION ; ABSCISIC-ACID ; DIVERSE ARRAY ; MODIFIER ; SNC1
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000457568200016
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	Chinese Academy of SciencesChinese Academy of Sciences [XDA08010105] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670186, 31471363]
作者邮箱	jinjb@ibcas.ac.cn
作品OA属性	hybrid
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19456
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Korea Res Inst Biosci & Biotechnol, Biol Resource Ctr, Jeongeup 56212, South Korea
推荐引用方式 GB/T 7714	Niu, De,Lin, Xiao-Li,Kong, Xiangxiong,et al. SIZ1-Mediated SUMOylation of TPR1 Suppresses Plant Immunity in Arabidopsis[J]. MOLECULAR PLANT,2019,12(2):215-228.
APA	Niu, De.,Lin, Xiao-Li.,Kong, Xiangxiong.,Qu, Gao-Ping.,Cai, Bin.,...&Jin, Jing Bo.(2019).SIZ1-Mediated SUMOylation of TPR1 Suppresses Plant Immunity in Arabidopsis.MOLECULAR PLANT,12(2),215-228.
MLA	Niu, De,et al."SIZ1-Mediated SUMOylation of TPR1 Suppresses Plant Immunity in Arabidopsis".MOLECULAR PLANT 12.2(2019):215-228.

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