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| mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ | |
Ding, Shunhua; Zhang, Yi1; Hu, Zhi1; Huang, Xiahe2; Zhang, Bohan1; Lu, Qingtao ; Wen, Xiaogang; Wang, Yingchun1,2; Lu, Congming3
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| 2019 | |
| Source Publication | MOLECULAR PLANT
 |
| ISSN | 1674-2052 |
| Volume | 12Issue:9Pages:1259-1277 |
| Abstract | RNA polymerase transcriptional pausing represents a major checkpoint in transcription in bacteria and metazoans, but it is unknown whether this phenomenon occurs in plant organelles. Here, we report that transcriptional pausing occurs in chloroplasts. We found that mTERF5 specifically and positively regulates the transcription of chloroplast psbEFLJ in Arabidopsis thaliana that encodes four key subunits of photo-system II. We found that mTERF5 causes the plastid-encoded RNA polymerase (PEP) complex to pause at psbEFLJ by binding to the +30 to +51 region of double-stranded DNA. Moreover, we revealed that mTERF5 interacts with pTAC6, an essential subunit of the PEP complex, although pTAC6 is not involved in the transcriptional pausing at psbEFLJ. We showed that mTERF5 recruits additional pTAC6 to the transcriptionally paused region of psbEFLJ, and the recruited pTAC6 proteins could be assembled into the PEP complex to regulate psbEFLJ transcription. Taken together, our findings shed light on the role of transcriptional pausing in chloroplast transcription in plants. |
| Keyword | mTERF5 transcriptional pausing transcription chloroplast psbEFLJ |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1016/j.molp.2019.05.007 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | NEGATIVE ELONGATION-FACTOR ; DOMAIN PROTEIN FUNCTIONS ; ENCODED RNA-POLYMERASE ; GENE-EXPRESSION ; PHOTOSYSTEM-I ; COMPLEX REVEALS ; ARABIDOPSIS ; TERMINATION ; PROMOTER ; DNA |
| WOS Research Area | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS ID | WOS:000483463400010 |
| Publisher | CELL PRESS |
| Subtype | Article |
| Publication Place | CAMBRIDGE |
| EISSN | 1752-9867 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730102] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; National Key Scientific Instrument and Equipment Development Project of China [2013YQ030595] |
| Corresponding Author Email | cmlu@sdau.edu.cn |
| OA | Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/19457 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 4.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China |
| Recommended Citation GB/T 7714 | Ding, Shunhua,Zhang, Yi,Hu, Zhi,et al. mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ[J]. MOLECULAR PLANT,2019,12(9):1259-1277. |
| APA | Ding, Shunhua.,Zhang, Yi.,Hu, Zhi.,Huang, Xiahe.,Zhang, Bohan.,...&Lu, Congming.(2019).mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ.MOLECULAR PLANT,12(9),1259-1277. |
| MLA | Ding, Shunhua,et al."mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ".MOLECULAR PLANT 12.9(2019):1259-1277. |
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| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S167420521930（3340KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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