mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ

doi:10.1016/j.molp.2019.05.007

IB-CAS > 中科院光生物学重点实验室

	mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ
	Ding, Shunhua ; Zhang, Yi 1; Hu, Zhi 1; Huang, Xiahe 2; Zhang, Bohan 1; Lu, Qingtao; Wen, Xiaogang ; Wang, Yingchun 1,2; Lu, Congming 3
	2019
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	12 期号:9 页码:1259-1277
摘要	RNA polymerase transcriptional pausing represents a major checkpoint in transcription in bacteria and metazoans, but it is unknown whether this phenomenon occurs in plant organelles. Here, we report that transcriptional pausing occurs in chloroplasts. We found that mTERF5 specifically and positively regulates the transcription of chloroplast psbEFLJ in Arabidopsis thaliana that encodes four key subunits of photo-system II. We found that mTERF5 causes the plastid-encoded RNA polymerase (PEP) complex to pause at psbEFLJ by binding to the +30 to +51 region of double-stranded DNA. Moreover, we revealed that mTERF5 interacts with pTAC6, an essential subunit of the PEP complex, although pTAC6 is not involved in the transcriptional pausing at psbEFLJ. We showed that mTERF5 recruits additional pTAC6 to the transcriptionally paused region of psbEFLJ, and the recruited pTAC6 proteins could be assembled into the PEP complex to regulate psbEFLJ transcription. Taken together, our findings shed light on the role of transcriptional pausing in chloroplast transcription in plants.
关键词	mTERF5 transcriptional pausing transcription chloroplast psbEFLJ
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2019.05.007
收录类别	SCI
语种	英语
WOS关键词	NEGATIVE ELONGATION-FACTOR ; DOMAIN PROTEIN FUNCTIONS ; ENCODED RNA-POLYMERASE ; GENE-EXPRESSION ; PHOTOSYSTEM-I ; COMPLEX REVEALS ; ARABIDOPSIS ; TERMINATION ; PROMOTER ; DNA
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000483463400010
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730102] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; National Key Scientific Instrument and Equipment Development Project of China [2013YQ030595]
作者邮箱	cmlu@sdau.edu.cn
作品OA属性	Bronze
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19457
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 4.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ding, Shunhua,Zhang, Yi,Hu, Zhi,et al. mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ[J]. MOLECULAR PLANT,2019,12(9):1259-1277.
APA	Ding, Shunhua.,Zhang, Yi.,Hu, Zhi.,Huang, Xiahe.,Zhang, Bohan.,...&Lu, Congming.(2019).mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ.MOLECULAR PLANT,12(9),1259-1277.
MLA	Ding, Shunhua,et al."mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ".MOLECULAR PLANT 12.9(2019):1259-1277.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
1-s2.0-S167420521930（3340KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文