IB-CAS  > 中科院光生物学重点实验室
mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ
Ding, Shunhua; Zhang, Yi1; Hu, Zhi1; Huang, Xiahe2; Zhang, Bohan1; Lu, Qingtao; Wen, Xiaogang; Wang, Yingchun1,2; Lu, Congming3
2019
Source PublicationMOLECULAR PLANT
ISSN1674-2052
Volume12Issue:9Pages:1259-1277
AbstractRNA polymerase transcriptional pausing represents a major checkpoint in transcription in bacteria and metazoans, but it is unknown whether this phenomenon occurs in plant organelles. Here, we report that transcriptional pausing occurs in chloroplasts. We found that mTERF5 specifically and positively regulates the transcription of chloroplast psbEFLJ in Arabidopsis thaliana that encodes four key subunits of photo-system II. We found that mTERF5 causes the plastid-encoded RNA polymerase (PEP) complex to pause at psbEFLJ by binding to the +30 to +51 region of double-stranded DNA. Moreover, we revealed that mTERF5 interacts with pTAC6, an essential subunit of the PEP complex, although pTAC6 is not involved in the transcriptional pausing at psbEFLJ. We showed that mTERF5 recruits additional pTAC6 to the transcriptionally paused region of psbEFLJ, and the recruited pTAC6 proteins could be assembled into the PEP complex to regulate psbEFLJ transcription. Taken together, our findings shed light on the role of transcriptional pausing in chloroplast transcription in plants.
KeywordmTERF5 transcriptional pausing transcription chloroplast psbEFLJ
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
DOI10.1016/j.molp.2019.05.007
Indexed BySCI
Language英语
WOS KeywordNEGATIVE ELONGATION-FACTOR ; DOMAIN PROTEIN FUNCTIONS ; ENCODED RNA-POLYMERASE ; GENE-EXPRESSION ; PHOTOSYSTEM-I ; COMPLEX REVEALS ; ARABIDOPSIS ; TERMINATION ; PROMOTER ; DNA
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000483463400010
PublisherCELL PRESS
SubtypeArticle
Publication PlaceCAMBRIDGE
EISSN1752-9867
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730102] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; National Key Scientific Instrument and Equipment Development Project of China [2013YQ030595]
Corresponding Author Emailcmlu@sdau.edu.cn
OABronze
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Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19457
Collection中科院光生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China
4.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Ding, Shunhua,Zhang, Yi,Hu, Zhi,et al. mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ[J]. MOLECULAR PLANT,2019,12(9):1259-1277.
APA Ding, Shunhua.,Zhang, Yi.,Hu, Zhi.,Huang, Xiahe.,Zhang, Bohan.,...&Lu, Congming.(2019).mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ.MOLECULAR PLANT,12(9),1259-1277.
MLA Ding, Shunhua,et al."mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ".MOLECULAR PLANT 12.9(2019):1259-1277.
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