Shifts of the nirS and nirK denitrifiers in different land use types and seasons in the Sanjiang Plain, China

doi:10.1002/jobm.201900192

	Shifts of the nirS and nirK denitrifiers in different land use types and seasons in the Sanjiang Plain, China
	Wang, Chunyong 1; Li, Juan 2; Wu, Yuntao 2; Song, Yi 1; Liu, Ruyin 1; Cao, Zicheng 1; Cui, Yanshan 1
	2019
发表期刊	JOURNAL OF BASIC MICROBIOLOGY
ISSN	0233-111X
卷号	59 期号:10 页码:1040-1048
摘要	Denitrification is a key nitrogen removal process that involves many denitrifying bacteria. In this study, the denitrification performance was estimated for soil samples from different land use types including farmland soil, restored wetland soil, and wetland soil. The quantitative real-time polymerase chain reaction results showed that the average abundance of nirS and nirK genes was notably affected by seasonal changes, increasing from 2.34 x 10(6) and 2.81 x 10(6) to 1.97 x 10(6) and 4.55 x 10(6) gene copies/g of dry soil, respectively, from autumn to spring. This suggests that the abundance of nirS and nirK denitrifiers in spring is higher than those in autumn. Furthermore, the abundance of nirS and nirK genes was higher in the farmland soil than in restored wetland soil and wetland soil in both seasons. According to the analyses of MiSeq sequencing of nirS and nirK genes, Halobacteriaceae could be used as a special strain to distinguish wetland soil from farmland soil and restored wetland soil. Furthermore, redundancy analysis indicated that the soil environmental variables of total carbon, total nitrogen, moisture content, and organic matter were the main factors affecting the community structures of nirS and nirK denitrifiers existing in wetland soil. These findings could contribute to understanding the differences in nirS and nirK denitrifiers between different land use types during seasonal changes.
关键词	denitrification denitrifying bacteria nirK nirS wetland
学科领域	Microbiology
DOI	10.1002/jobm.201900192
收录类别	SCI
语种	英语
WOS关键词	16S RIBOSOMAL-RNA ; COMMUNITY STRUCTURE ; AGRICULTURAL-DEVELOPMENT ; ORGANIC-MATTER ; N2O PRODUCTION ; NITROGEN ; BACTERIA ; WETLANDS ; CARBON ; CONVERSION
WOS研究方向	Microbiology
WOS记录号	WOS:000522248200007
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1521-4028
资助机构	National Key R&D Program of China [2016YFC0500408]
作者邮箱	cuiyanshan@ucas.ac.cn
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19501
专题	植被与环境变化国家重点实验室
作者单位	1.Univ Chinese Acad Sci, Coll Resources & Environm, Huaibei Village 380, Beijing 101408, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chunyong,Li, Juan,Wu, Yuntao,et al. Shifts of the nirS and nirK denitrifiers in different land use types and seasons in the Sanjiang Plain, China[J]. JOURNAL OF BASIC MICROBIOLOGY,2019,59(10):1040-1048.
APA	Wang, Chunyong.,Li, Juan.,Wu, Yuntao.,Song, Yi.,Liu, Ruyin.,...&Cui, Yanshan.(2019).Shifts of the nirS and nirK denitrifiers in different land use types and seasons in the Sanjiang Plain, China.JOURNAL OF BASIC MICROBIOLOGY,59(10),1040-1048.
MLA	Wang, Chunyong,et al."Shifts of the nirS and nirK denitrifiers in different land use types and seasons in the Sanjiang Plain, China".JOURNAL OF BASIC MICROBIOLOGY 59.10(2019):1040-1048.

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