Large-scale Identification and Time -course Quantification of Ubiquitylation Events During Maize Seedling De -etiolation

doi:10.1016/j.gpb.2018.05.005

IB-CAS > 中科院光生物学重点实验室

	Large-scale Identification and Time -course Quantification of Ubiquitylation Events During Maize Seedling De -etiolation
	Wang, Yue-Feng ; Chao, Qing ; Li, Zhe 2; Lu, Tian-Cong ; Zheng, Hai-Yan ; Zhao, Cai-Feng ; Shen, Zhuo ; Li, Xiao-Hui ; Wang, Bai-Chen
	2019
发表期刊	GENOMICS PROTEOMICS & BIOINFORMATICS
ISSN	1672-0229
卷号	17 期号:6 页码:603-622
摘要	The ubiquitin system is crucial for the development and fitness of higher plants. De-etiolation, during which green plants initiate photomorphogenesis and establish autotrophy, is a dramatic and complicated process that is tightly regulated by a massive number of ubiquitylation/de-ubiquitylation events. Here we present site-specific quantitative proteomic data for the ubiquitylomes of de-etiolating seedling leaves of Zea mays L. (exposed to light for 1, 6, or 12 h) achieved through immunoprecipitation-based high-resolution mass spectrometry (MS). Through the integrated analysis of multiple ubiquitylomes, we identified and quantified 1926 unique ubiquitylation sites corresponding to 1053 proteins. We analyzed these sites and found five potential ubiquitylation motifs, KA, AXK, KXG, AK, and TK. Time-course studies revealed that the ubiquitylation levels of 214 sites corresponding to 173 proteins were highly correlated across two replicate MS experiments, and significant alterations in the ubiquitylation levels of 78 sites (fold change >1.5) were detected after de-etiolation for 12 h. The majority of the ubiquitylated sites we identified corresponded to substrates involved in protein and DNA metabolism, such as ribosomes and histones. Meanwhile, multiple ubiquitylation sites were detected in proteins whose functions reflect the major physiological changes that occur during plant de-etiolation, such as hormone synthesis/signaling proteins, key C4 photosynthetic enzymes, and light signaling proteins. This study on the ubiquitylome of the maize seedling leaf is the first attempt ever to study the ubiquitylome of a C4 plant and provides the proteomic basis for elucidating the role of ubiquitylation during plant de-etiolation.
关键词	Maize seedling leaf De-etiolation Ubiquitylation C4 photosynthetic enzymes Ribosome
学科领域	Genetics & Heredity
DOI	10.1016/j.gpb.2018.05.005
收录类别	SCI
语种	英语
WOS关键词	PHOSPHOENOLPYRUVATE CARBOXYLASE ; LYSINE UBIQUITINATION ; PROTEIN-DEGRADATION ; PROTEASOME SYSTEM ; GENE-EXPRESSION ; 26S PROTEASOME ; LIGHT ; MONOUBIQUITINATION ; PROTEOMES ; SYNTHASE
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000535175900005
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	2210-3244
资助机构	National Key R&D Program of China [2016YFD0101003] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA08010206] ; Agricultural Science and Technology Innovation Program of Jilin Province [CXGC2017JQ019]
作者邮箱	xhli@cjaas.com ; wangbc@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19516
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, CAS Key Lab Photobiol, Beijing 100093, Peoples R China 2.Jilin Acad Agr Sci, Crop Germplasm Resources Inst, Gongzhuling 136100, Peoples R China 3.Precis Sci Beijing Co, Beijing 100085, Peoples R China 4.Univ Sci & Technol Beijing, Adv Biotechnol & Applicat Res Ctr, Sch Chem & Biol Engn, Beijing 100083, Peoples R China 5.Rutgers State Univ, Ctr Adv Biotechnol & Med, Biol Mass Spectrometry Facil, Piscataway, NJ 08855 USA 6.Grad Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yue-Feng,Chao, Qing,Li, Zhe,et al. Large-scale Identification and Time -course Quantification of Ubiquitylation Events During Maize Seedling De -etiolation[J]. GENOMICS PROTEOMICS & BIOINFORMATICS,2019,17(6):603-622.
APA	Wang, Yue-Feng.,Chao, Qing.,Li, Zhe.,Lu, Tian-Cong.,Zheng, Hai-Yan.,...&Wang, Bai-Chen.(2019).Large-scale Identification and Time -course Quantification of Ubiquitylation Events During Maize Seedling De -etiolation.GENOMICS PROTEOMICS & BIOINFORMATICS,17(6),603-622.
MLA	Wang, Yue-Feng,et al."Large-scale Identification and Time -course Quantification of Ubiquitylation Events During Maize Seedling De -etiolation".GENOMICS PROTEOMICS & BIOINFORMATICS 17.6(2019):603-622.

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