SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola

doi:10.1111/pce.13456

	SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola
	Zhao, Haixia 1; Wang, Liangsheng; Zhao, Fang-Jie ; Wu, Longhua 3; Liu, Anna ; Xu, Wenzhong
	2019
发表期刊	PLANT CELL AND ENVIRONMENT
ISSN	0140-7791
卷号	42 期号:4 页码:1112-1124
摘要	Sedum plumbizincicola is able to hyperaccumulate cadmium (Cd), a nonessential and highly toxic metal, in the above-ground tissues, but the mechanisms for its Cd hypertolerance are not fully understood. Here, we show that the heavy metal ATPase 1 (SpHMA1) of S. plumbizincicola plays an important role in chloroplast Cd detoxification. Compared with the HMA1 ortholog in the Cd nonhyperaccumulating ecotype of Sedum alfredii, the expression of SpHMA1 in the leaves of S. plumbizincicola was >200 times higher. Heterologous expression of SpHMA1 in Saccharomyces cerevisiae increased Cd sensitivity and Cd transport activity in the yeast cells. The SpHMA1 protein was localized to the chloroplast envelope. SpHMA1 RNA interference transgenic plants and CRISPR/Cas9-induced mutant lines showed significantly increased Cd accumulation in the chloroplasts compared with wild-type plants. Chlorophyll fluorescence imaging analysis revealed that the photosystem II of SpHMA1 knockdown and knockout lines suffered from a much higher degree of Cd toxicity than wild type. Taken together, these results suggest that SpHMA1 functions as a chloroplast Cd exporter and protects photosynthesis by preventing Cd accumulation in the chloroplast in S. plumbizincicola and hyperexpression of SpHMA1 is an important component contributing to Cd hypertolerance in S. plumbizincicola.
关键词	cadmium (Cd) chloroplast CRISPR/Cas9 heavy metal ATPase 1 (HMA1) hyperaccumulator Sedum plumbizincicola transporter
学科领域	Plant Sciences
DOI	10.1111/pce.13456
收录类别	SCI
语种	英语
WOS关键词	P-TYPE ATPASE ; CHLOROPHYLL FLUORESCENCE ; INDUCED INHIBITION ; METAL HOMEOSTASIS ; PHOTOSYSTEM-II ; ARABIDOPSIS ; ZINC ; TOLERANCE ; PHOTOSYNTHESIS ; ACCUMULATION
WOS研究方向	Plant Sciences
WOS记录号	WOS:000462360300003
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-3040
资助机构	National Key R&D Program of China [2017YFD0800900]
作者邮箱	xuwzh@ibcas.ac.cn
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19530
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China 4.Chinese Acad Sci, Key Lab Soil Environm & Pollut Remediat, Inst Soil Sci, Nanjing, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,et al. SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola[J]. PLANT CELL AND ENVIRONMENT,2019,42(4):1112-1124.
APA	Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,Wu, Longhua,Liu, Anna,&Xu, Wenzhong.(2019).SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola.PLANT CELL AND ENVIRONMENT,42(4),1112-1124.
MLA	Zhao, Haixia,et al."SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola".PLANT CELL AND ENVIRONMENT 42.4(2019):1112-1124.

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