IB-CAS  > 中科院北方资源植物重点实验室
SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola
Zhao, Haixia1; Wang, Liangsheng; Zhao, Fang-Jie; Wu, Longhua3; Liu, Anna; Xu, Wenzhong
2019
Source PublicationPLANT CELL AND ENVIRONMENT
ISSN0140-7791
Volume42Issue:4Pages:1112-1124
AbstractSedum plumbizincicola is able to hyperaccumulate cadmium (Cd), a nonessential and highly toxic metal, in the above-ground tissues, but the mechanisms for its Cd hypertolerance are not fully understood. Here, we show that the heavy metal ATPase 1 (SpHMA1) of S. plumbizincicola plays an important role in chloroplast Cd detoxification. Compared with the HMA1 ortholog in the Cd nonhyperaccumulating ecotype of Sedum alfredii, the expression of SpHMA1 in the leaves of S. plumbizincicola was >200 times higher. Heterologous expression of SpHMA1 in Saccharomyces cerevisiae increased Cd sensitivity and Cd transport activity in the yeast cells. The SpHMA1 protein was localized to the chloroplast envelope. SpHMA1 RNA interference transgenic plants and CRISPR/Cas9-induced mutant lines showed significantly increased Cd accumulation in the chloroplasts compared with wild-type plants. Chlorophyll fluorescence imaging analysis revealed that the photosystem II of SpHMA1 knockdown and knockout lines suffered from a much higher degree of Cd toxicity than wild type. Taken together, these results suggest that SpHMA1 functions as a chloroplast Cd exporter and protects photosynthesis by preventing Cd accumulation in the chloroplast in S. plumbizincicola and hyperexpression of SpHMA1 is an important component contributing to Cd hypertolerance in S. plumbizincicola.
Keywordcadmium (Cd) chloroplast CRISPR/Cas9 heavy metal ATPase 1 (HMA1) hyperaccumulator Sedum plumbizincicola transporter
Subject AreaPlant Sciences
DOI10.1111/pce.13456
Indexed BySCI
Language英语
WOS KeywordP-TYPE ATPASE ; CHLOROPHYLL FLUORESCENCE ; INDUCED INHIBITION ; METAL HOMEOSTASIS ; PHOTOSYSTEM-II ; ARABIDOPSIS ; ZINC ; TOLERANCE ; PHOTOSYNTHESIS ; ACCUMULATION
WOS Research AreaPlant Sciences
WOS IDWOS:000462360300003
PublisherWILEY
SubtypeArticle
Publication PlaceHOBOKEN
EISSN1365-3040
Funding OrganizationNational Key R&D Program of China [2017YFD0800900]
Corresponding Author Emailxuwzh@ibcas.ac.cn
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19530
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
3.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China
4.Chinese Acad Sci, Key Lab Soil Environm & Pollut Remediat, Inst Soil Sci, Nanjing, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,et al. SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola[J]. PLANT CELL AND ENVIRONMENT,2019,42(4):1112-1124.
APA Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,Wu, Longhua,Liu, Anna,&Xu, Wenzhong.(2019).SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola.PLANT CELL AND ENVIRONMENT,42(4),1112-1124.
MLA Zhao, Haixia,et al."SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola".PLANT CELL AND ENVIRONMENT 42.4(2019):1112-1124.
Files in This Item:
File Name/Size DocType Version Access License
Applet_2021-12-13_16(1665KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Haixia]'s Articles
[Wang, Liangsheng]'s Articles
[Zhao, Fang-Jie]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Haixia]'s Articles
[Wang, Liangsheng]'s Articles
[Zhao, Fang-Jie]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Haixia]'s Articles
[Wang, Liangsheng]'s Articles
[Zhao, Fang-Jie]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Applet_2021-12-13_163935990658953.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.