Knowledge Management System Of Institute Of Botany,CAS
| SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola | |
Zhao, Haixia1; Wang, Liangsheng ; Zhao, Fang-Jie; Wu, Longhua3; Liu, Anna; Xu, Wenzhong
| |
| 2019 | |
| Source Publication | PLANT CELL AND ENVIRONMENT
 |
| ISSN | 0140-7791 |
| Volume | 42Issue:4Pages:1112-1124 |
| Abstract | Sedum plumbizincicola is able to hyperaccumulate cadmium (Cd), a nonessential and highly toxic metal, in the above-ground tissues, but the mechanisms for its Cd hypertolerance are not fully understood. Here, we show that the heavy metal ATPase 1 (SpHMA1) of S. plumbizincicola plays an important role in chloroplast Cd detoxification. Compared with the HMA1 ortholog in the Cd nonhyperaccumulating ecotype of Sedum alfredii, the expression of SpHMA1 in the leaves of S. plumbizincicola was >200 times higher. Heterologous expression of SpHMA1 in Saccharomyces cerevisiae increased Cd sensitivity and Cd transport activity in the yeast cells. The SpHMA1 protein was localized to the chloroplast envelope. SpHMA1 RNA interference transgenic plants and CRISPR/Cas9-induced mutant lines showed significantly increased Cd accumulation in the chloroplasts compared with wild-type plants. Chlorophyll fluorescence imaging analysis revealed that the photosystem II of SpHMA1 knockdown and knockout lines suffered from a much higher degree of Cd toxicity than wild type. Taken together, these results suggest that SpHMA1 functions as a chloroplast Cd exporter and protects photosynthesis by preventing Cd accumulation in the chloroplast in S. plumbizincicola and hyperexpression of SpHMA1 is an important component contributing to Cd hypertolerance in S. plumbizincicola. |
| Keyword | cadmium (Cd) chloroplast CRISPR/Cas9 heavy metal ATPase 1 (HMA1) hyperaccumulator Sedum plumbizincicola transporter |
| Subject Area | Plant Sciences |
| DOI | 10.1111/pce.13456 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | P-TYPE ATPASE ; CHLOROPHYLL FLUORESCENCE ; INDUCED INHIBITION ; METAL HOMEOSTASIS ; PHOTOSYSTEM-II ; ARABIDOPSIS ; ZINC ; TOLERANCE ; PHOTOSYNTHESIS ; ACCUMULATION |
| WOS Research Area | Plant Sciences |
| WOS ID | WOS:000462360300003 |
| Publisher | WILEY |
| Subtype | Article |
| Publication Place | HOBOKEN |
| EISSN | 1365-3040 |
| Funding Organization | National Key R&D Program of China [2017YFD0800900] |
| Corresponding Author Email | xuwzh@ibcas.ac.cn |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/19530 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China 4.Chinese Acad Sci, Key Lab Soil Environm & Pollut Remediat, Inst Soil Sci, Nanjing, Jiangsu, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,et al. SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola[J]. PLANT CELL AND ENVIRONMENT,2019,42(4):1112-1124. |
| APA | Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,Wu, Longhua,Liu, Anna,&Xu, Wenzhong.(2019).SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola.PLANT CELL AND ENVIRONMENT,42(4),1112-1124. |
| MLA | Zhao, Haixia,et al."SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola".PLANT CELL AND ENVIRONMENT 42.4(2019):1112-1124. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Applet_2021-12-13_16（1665KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment