Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis

doi:10.1111/tpj.14302

IB-CAS > 中科院光生物学重点实验室

	Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis
	Liu, Jin ; Sun, Zheng 1; Mao, Xuemei ; Gerken, Henri 2; Wang, Xiaofei ; Yang, Wenqiang3
	2019
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	98 期号:6 页码:1060-1077
摘要	Chromochloris zofingiensis, featured due to its capability to simultaneously synthesize triacylglycerol (TAG) and astaxanthin, is emerging as a leading candidate alga for production uses. To better understand the oleaginous mechanism of this alga, we conducted a multiomics analysis by systematically integrating time-resolved transcriptomes, lipidomes and metabolomes in response to nitrogen deprivation. The data analysis unraveled the distinct mechanism of TAG accumulation, which involved coordinated stimulation of multiple biological processes including supply of energy and reductants, carbon reallocation from protein and starch, and 'pushing' and 'pulling' carbon to TAG synthesis. Unlike the model alga Chlamydomonas, de novo fatty acid synthesis in C. zofingiensis was promoted, together with enhanced turnover of both glycolipids and phospholipids, supporting the drastic need of acyls for TAG assembly. Moreover, genomewide analysis identified many key functional enzymes and transcription factors that had engineering potential for TAG modulation. Two genes encoding glycerol-3-phosphate acyltransferase (GPAT), the first committed enzyme for TAG assembly, were found in the C. zofingiensis genome; in vivo functional characterization revealed that extrachloroplastic GPAT instead of chloroplastic GPAT played a central role in TAG synthesis. These findings illuminate distinct oleaginousness mechanisms in C. zofingiensis and pave the way towards rational manipulation of this alga to becone an emerging model for trait improvements.
关键词	Chromochloris zofingiensis glycerol-3-phosphate acyltransferase oleaginousness multiomics trait improvement triacylglycerol
学科领域	Plant Sciences
DOI	10.1111/tpj.14302
收录类别	SCI
语种	英语
WOS关键词	MICROALGA CHLORELLA-ZOFINGIENSIS ; PENTOSE-PHOSPHATE PATHWAY ; CHLAMYDOMONAS-REINHARDTII ; DIACYLGLYCEROL ACYLTRANSFERASES ; NITROGEN DEPRIVATION ; LIPID-ACCUMULATION ; ACID ACYLTRANSFERASE ; PHYTOENE DESATURASE ; BIOFUEL PRODUCTION ; OIL SYNTHESIS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000473644100009
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Youth Thousand Talents Program ; Peking University CCUS project - BHP Billiton ; Double-First Class Initiative of Peking University
作者邮箱	gjinliu@pku.edu.cn
作品OA属性	Bronze
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19544
专题	中科院光生物学重点实验室
作者单位	1.Peking Univ, Coll Engn, Lab Algae Biotechnol & Innovat, Beijing 100871, Peoples R China 2.Shanghai Ocean Univ, Minist Sci & Technol, Int Res Ctr Marine Biosci, Shanghai 201306, Peoples R China 3.Arizona State Univ Polytech Campus, Sch Sustainable Engn & Built Environm, Mesa, AZ 85212 USA 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jin,Sun, Zheng,Mao, Xuemei,et al. Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis[J]. PLANT JOURNAL,2019,98(6):1060-1077.
APA	Liu, Jin,Sun, Zheng,Mao, Xuemei,Gerken, Henri,Wang, Xiaofei,&Yang, Wenqiang.(2019).Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis.PLANT JOURNAL,98(6),1060-1077.
MLA	Liu, Jin,et al."Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis".PLANT JOURNAL 98.6(2019):1060-1077.

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