Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity

doi:10.1016/j.molp.2019.03.012

	Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity
	Liu, Cheng 1; Cui, Dayong 2; Zhao, Jingbo ; Liu, Na 3; Wang, Bo 4; Liu, Jing ; Xu, Enjun ; Hu, Zhubing 5; Ren, Dongtao 4; Tang, Dingzhong 3; Hu, Yuxin6
	2019
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	12 期号:7 页码:967-983
摘要	Plants utilize intracellular nucleotide-binding leucine-rich repeat domain-containing receptors (NLRs) to recognize pathogen effectors and induce a robust defense response named effector-triggered immunity (ETI). The Arabidopsis NLR protein HOPZ-ACTIVATED RESISTANCE 1 (ZAR1) forms a precomplex with HOPZ-ETI-DEFICIENT 1 (ZED1), a receptor-like cytoplasmic kinase (RLCK) XII-2 subfamily member, to recognize the Pseudomonas syringae effector HopZ1a. We previously described a dominant mutant of Arabidopsis ZED1, zed1-D, which displays temperature-sensitive autoimmunity in a ZAR1-dependent manner. Here, we report that the RLCKs SUPPRESSOR OF ZED1-D1 (SZE1) and SZE2 associate with the ZAR1-ZED1 complex and are required for the ZED1-D-activated autoimmune response and HopZ1a-triggered immunity. We show that SZE1 but not SZE2 has autophosphorylation activity, and that the N-terminal myristoylation of both SZE1 and SZE2 is critical for their plasma membrane localization and ZED1-D-activated autoimmunity. Furthermore, we demonstrate that SZE1 and SZE2 both interact with ZAR1 to form a functional complex and are required for resistance against P. syringae pv. tomato DC3000 expressing HopZ1a. We also provide evidence that SZE1 and SZE2 interact with HopZ1a and function together with ZED1 to change the intramolecular interactions of ZAR1, leading to its activation. Taken together, our results reveal SZE1 and SZE2 as critical signaling components of HopZ1a-triggered immunity.
关键词	Arabidopsis RLCK SZE-ZAR1-ZED1 complex HopZ1a immune response
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2019.03.012
收录类别	SCI
语种	英语
WOS关键词	DISEASE RESISTANCE PROTEIN ; FLAX RUST ; PLANT ; ACTIVATION ; RECOGNITION ; PERCEPTION ; FAMILY ; DOMAIN ; MYRISTOYLATION ; BIOSYNTHESIS
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000473296400007
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471160] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDB27030102]
作者邮箱	huyuxin@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19546
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qilu Normal Univ, Sch Life Sci, Jinan 250200, Shandong, Peoples R China 4.Fujian Agr & Forestry Univ, Plant Immun Ctr, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou 350002, Peoples R China 5.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 6.Henan Univ, Dept Biol, State Key Lab Cotton Biol, Inst Plant Stress Biol, Kaifeng 475001, Peoples R China 7.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Liu, Cheng,Cui, Dayong,Zhao, Jingbo,et al. Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity[J]. MOLECULAR PLANT,2019,12(7):967-983.
APA	Liu, Cheng.,Cui, Dayong.,Zhao, Jingbo.,Liu, Na.,Wang, Bo.,...&Hu, Yuxin.(2019).Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity.MOLECULAR PLANT,12(7),967-983.
MLA	Liu, Cheng,et al."Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity".MOLECULAR PLANT 12.7(2019):967-983.

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