Knowledge Management System Of Institute Of Botany,CAS
| Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity | |
Liu, Cheng1; Cui, Dayong2; Zhao, Jingbo; Liu, Na3; Wang, Bo4; Liu, Jing; Xu, Enjun; Hu, Zhubing5; Ren, Dongtao4; Tang, Dingzhong3; Hu, Yuxin6
| |
| 2019 | |
| Source Publication | MOLECULAR PLANT
 |
| ISSN | 1674-2052 |
| Volume | 12Issue:7Pages:967-983 |
| Abstract | Plants utilize intracellular nucleotide-binding leucine-rich repeat domain-containing receptors (NLRs) to recognize pathogen effectors and induce a robust defense response named effector-triggered immunity (ETI). The Arabidopsis NLR protein HOPZ-ACTIVATED RESISTANCE 1 (ZAR1) forms a precomplex with HOPZ-ETI-DEFICIENT 1 (ZED1), a receptor-like cytoplasmic kinase (RLCK) XII-2 subfamily member, to recognize the Pseudomonas syringae effector HopZ1a. We previously described a dominant mutant of Arabidopsis ZED1, zed1-D, which displays temperature-sensitive autoimmunity in a ZAR1-dependent manner. Here, we report that the RLCKs SUPPRESSOR OF ZED1-D1 (SZE1) and SZE2 associate with the ZAR1-ZED1 complex and are required for the ZED1-D-activated autoimmune response and HopZ1a-triggered immunity. We show that SZE1 but not SZE2 has autophosphorylation activity, and that the N-terminal myristoylation of both SZE1 and SZE2 is critical for their plasma membrane localization and ZED1-D-activated autoimmunity. Furthermore, we demonstrate that SZE1 and SZE2 both interact with ZAR1 to form a functional complex and are required for resistance against P. syringae pv. tomato DC3000 expressing HopZ1a. We also provide evidence that SZE1 and SZE2 interact with HopZ1a and function together with ZED1 to change the intramolecular interactions of ZAR1, leading to its activation. Taken together, our results reveal SZE1 and SZE2 as critical signaling components of HopZ1a-triggered immunity. |
| Keyword | Arabidopsis RLCK SZE-ZAR1-ZED1 complex HopZ1a immune response |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1016/j.molp.2019.03.012 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | DISEASE RESISTANCE PROTEIN ; FLAX RUST ; PLANT ; ACTIVATION ; RECOGNITION ; PERCEPTION ; FAMILY ; DOMAIN ; MYRISTOYLATION ; BIOSYNTHESIS |
| WOS Research Area | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS ID | WOS:000473296400007 |
| Publisher | CELL PRESS |
| Subtype | Article |
| Publication Place | CAMBRIDGE |
| EISSN | 1752-9867 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471160] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDB27030102] |
| Corresponding Author Email | huyuxin@ibcas.ac.cn |
| OA | Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/19546 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qilu Normal Univ, Sch Life Sci, Jinan 250200, Shandong, Peoples R China 4.Fujian Agr & Forestry Univ, Plant Immun Ctr, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou 350002, Peoples R China 5.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 6.Henan Univ, Dept Biol, State Key Lab Cotton Biol, Inst Plant Stress Biol, Kaifeng 475001, Peoples R China 7.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Cheng,Cui, Dayong,Zhao, Jingbo,et al. Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity[J]. MOLECULAR PLANT,2019,12(7):967-983. |
| APA | Liu, Cheng.,Cui, Dayong.,Zhao, Jingbo.,Liu, Na.,Wang, Bo.,...&Hu, Yuxin.(2019).Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity.MOLECULAR PLANT,12(7),967-983. |
| MLA | Liu, Cheng,et al."Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity".MOLECULAR PLANT 12.7(2019):967-983. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Applet_2021-12-9_163（5784KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment