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Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity
Liu, Cheng1; Cui, Dayong2; Zhao, Jingbo; Liu, Na3; Wang, Bo4; Liu, Jing; Xu, Enjun; Hu, Zhubing5; Ren, Dongtao4; Tang, Dingzhong3; Hu, Yuxin6
2019
Source PublicationMOLECULAR PLANT
ISSN1674-2052
Volume12Issue:7Pages:967-983
AbstractPlants utilize intracellular nucleotide-binding leucine-rich repeat domain-containing receptors (NLRs) to recognize pathogen effectors and induce a robust defense response named effector-triggered immunity (ETI). The Arabidopsis NLR protein HOPZ-ACTIVATED RESISTANCE 1 (ZAR1) forms a precomplex with HOPZ-ETI-DEFICIENT 1 (ZED1), a receptor-like cytoplasmic kinase (RLCK) XII-2 subfamily member, to recognize the Pseudomonas syringae effector HopZ1a. We previously described a dominant mutant of Arabidopsis ZED1, zed1-D, which displays temperature-sensitive autoimmunity in a ZAR1-dependent manner. Here, we report that the RLCKs SUPPRESSOR OF ZED1-D1 (SZE1) and SZE2 associate with the ZAR1-ZED1 complex and are required for the ZED1-D-activated autoimmune response and HopZ1a-triggered immunity. We show that SZE1 but not SZE2 has autophosphorylation activity, and that the N-terminal myristoylation of both SZE1 and SZE2 is critical for their plasma membrane localization and ZED1-D-activated autoimmunity. Furthermore, we demonstrate that SZE1 and SZE2 both interact with ZAR1 to form a functional complex and are required for resistance against P. syringae pv. tomato DC3000 expressing HopZ1a. We also provide evidence that SZE1 and SZE2 interact with HopZ1a and function together with ZED1 to change the intramolecular interactions of ZAR1, leading to its activation. Taken together, our results reveal SZE1 and SZE2 as critical signaling components of HopZ1a-triggered immunity.
KeywordArabidopsis RLCK SZE-ZAR1-ZED1 complex HopZ1a immune response
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
DOI10.1016/j.molp.2019.03.012
Indexed BySCI
Language英语
WOS KeywordDISEASE RESISTANCE PROTEIN ; FLAX RUST ; PLANT ; ACTIVATION ; RECOGNITION ; PERCEPTION ; FAMILY ; DOMAIN ; MYRISTOYLATION ; BIOSYNTHESIS
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000473296400007
PublisherCELL PRESS
SubtypeArticle
Publication PlaceCAMBRIDGE
EISSN1752-9867
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471160] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDB27030102]
Corresponding Author Emailhuyuxin@ibcas.ac.cn
OABronze
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19546
Collection中科院植物分子生理学重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qilu Normal Univ, Sch Life Sci, Jinan 250200, Shandong, Peoples R China
4.Fujian Agr & Forestry Univ, Plant Immun Ctr, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou 350002, Peoples R China
5.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China
6.Henan Univ, Dept Biol, State Key Lab Cotton Biol, Inst Plant Stress Biol, Kaifeng 475001, Peoples R China
7.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Liu, Cheng,Cui, Dayong,Zhao, Jingbo,et al. Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity[J]. MOLECULAR PLANT,2019,12(7):967-983.
APA Liu, Cheng.,Cui, Dayong.,Zhao, Jingbo.,Liu, Na.,Wang, Bo.,...&Hu, Yuxin.(2019).Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity.MOLECULAR PLANT,12(7),967-983.
MLA Liu, Cheng,et al."Two Arabidopsis Receptor-like Cytoplasmic Kinases SZE1 and SZE2 Associate with the ZAR1-ZED1 Complex and Are Required for Effector-Triggered Immunity".MOLECULAR PLANT 12.7(2019):967-983.
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