The study of transcriptome sequencing for flower coloration in different anthesis stages of alpine ornamental herb (Meconopsis 'Lingholm')

doi:10.1016/j.gene.2018.12.017

	The study of transcriptome sequencing for flower coloration in different anthesis stages of alpine ornamental herb (Meconopsis 'Lingholm')
	Qu, Yan ; Ou, Zhi ; Yang, Fu-sheng ; Wang, Shu ; Peng, Jiansong
	2019
发表期刊	GENE
ISSN	0378-1119
卷号	689 页码:220-226
摘要	Meconopsis (Papaveraceae) is an interesting alpine herb, mainly distributed in the mountainous area of southwest China and high altitude zone in Tibetan-Himalaya. Different Meconopsis species showed a flower color alteration in different anthesis stages, Meconopsis `Lingholm' is one of the localized species whose petal color changes from purple to blue during the flowering process. In general, the blue color flower is a rare kind, and usually hard to cultivate artificially. The molecular mechanism of flower color formation and color alteration of alpine flowers were reported by many research workers. To find critical genes that regulate Meconopsis `Lingholm' color alteration and the mechanism of environmental adaptation, the current study performed transcriptome sequencing by using Meconopsis `Lingholm' petals from different anthesis stages. There were totally 91,615 unigenes obtained from 31.4 Gb sequencing data, and differentially expressed genes between two consecutive flowering stages were obtained. Bioinformatics studies showed genes regulating petal color alteration were activated. Moreover, the functional analysis showed that Meconopsis `Lingholm' showed a stress response to mechanical damage, non-biological stimulation and water deficiency in the bud stage, as well as showed a stress response to the cold from cracking stage to blooming stage. Furthermore, RNA-Seq results were verified using nine randomly selected genes by qPCR, which showed same expression trend with sequencing results. During this study, 20 candidate genes identified for further studies, which included five petal color related genes and 15 environmental response genes.
关键词	Meconopsis 'Linghohn' Blue petal Transcriptome sequencing Flower color alteration Anthocyanidin Heat shock protein
学科领域	Genetics & Heredity
DOI	10.1016/j.gene.2018.12.017
收录类别	SCI
语种	英语
WOS关键词	HIMALAYAN POPPY ; ANTHOCYANINS ; TEMPERATURE ; PLANT ; BIOSYNTHESIS ; PAPAVERACEAE ; STABILITY ; PATHWAY
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000459216900027
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	1879-0038
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31460218, 31570197] ; Key Disciplines Project (Landscape Architecture Ph.D) of Yunnan Education Department [2011(16)] ; Joint Special Program for Agricultural Basic Research of Yunnan Province [2017FG001(-68)]
作者邮箱	swallow@zoology.ubc.ca
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19562
专题	系统与进化植物学国家重点实验室
作者单位	1.Southwest Forestry Univ, State Forestry Adm China, Engn & Technol Res Ctr Landscape Architecture, Kunming 650224, Yunnan, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Qu, Yan,Ou, Zhi,Yang, Fu-sheng,et al. The study of transcriptome sequencing for flower coloration in different anthesis stages of alpine ornamental herb (Meconopsis 'Lingholm')[J]. GENE,2019,689:220-226.
APA	Qu, Yan,Ou, Zhi,Yang, Fu-sheng,Wang, Shu,&Peng, Jiansong.(2019).The study of transcriptome sequencing for flower coloration in different anthesis stages of alpine ornamental herb (Meconopsis 'Lingholm').GENE,689,220-226.
MLA	Qu, Yan,et al."The study of transcriptome sequencing for flower coloration in different anthesis stages of alpine ornamental herb (Meconopsis 'Lingholm')".GENE 689(2019):220-226.

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