Knowledge Management System Of Institute Of Botany,CAS
| Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing | |
Zhang, Yingchun1; Chi, Xu3; Feng, Laibao2; Wu, Xingwen1; Qi, Xiaoquan1
| |
| 2019 | |
| 发表期刊 | BIOTECHNIQUES
 |
| ISSN | 0736-6205 |
| 卷号 | 67期号:6页码:294-298 |
| 摘要 | The CRISPR/Cas9 system is an efficient gene-editing method, but it is difficult to obtain mutants for some specific species and special genome structures. A previously reported multiplexed, semi-nested PCR target-enrichment approach, which does not rely on transgenic technology, has been shown to be an effective and affordable strategy for the discovery of rare mutations in a large sodium azide-induced rice population. However, this strategy has the potential for further optimization. Here, we describe an improved multiplex semi-nested PCR target-enrichment strategy with simplified processing procedures, reduced false-positive rates and increased mutation detection frequency (1 mutation/73 Kb). |
| 关键词 | dilution improvement multiplexed target enrichment mutants next generation sequencing |
| 学科领域 | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| DOI | 10.2144/btn-2019-0001 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | REVERSE ; RNA ; ENDONUCLEASES ; ARABIDOPSIS ; DISCOVERY ; WHEAT |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000507374000008 |
| 出版者 | FUTURE SCI LTD |
| 文献子类 | Article |
| 出版地 | LONDON |
| EISSN | 1940-9818 |
| 资助机构 | National Transgenic Megaproject of China [2016ZX08009001-006] |
| 作者邮箱 | xqi@ibcas.ac.cn |
| 作品OA属性 | gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19565 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yingchun,Chi, Xu,Feng, Laibao,et al. Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing[J]. BIOTECHNIQUES,2019,67(6):294-298. |
| APA | Zhang, Yingchun,Chi, Xu,Feng, Laibao,Wu, Xingwen,&Qi, Xiaoquan.(2019).Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing.BIOTECHNIQUES,67(6),294-298. |
| MLA | Zhang, Yingchun,et al."Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing".BIOTECHNIQUES 67.6(2019):294-298. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| btn-2019-0001.pdf（1362KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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