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| Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation | |
Chai, Fengmei1,2; Liu, Wenwen1,2; Xiang, Yue2; Meng, Xianbin3; Sun, Xiaoming1; Cheng, Cheng2; Liu, Guotian4; Duan, Lixin5; Xin, Haiping; Li, Shaohua1
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| 2019 | |
| Source Publication | HORTICULTURE RESEARCH
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| ISSN | 2052-7276 |
| Volume | 6 |
| Abstract | Vitis amurensis is a wild Vitis plant that can withstand extreme cold temperatures. However, the accumulation of metabolites during cold acclimation (CA) in V. amurensis remains largely unknown. In this study, plantlets of V amurensis and V vinifera cv. Muscat of Hamburg were treated at 4 degrees C for 24 and 72 h, and changes of metabolites in leaves were detected by gas chromatography coupled with time-of-flight mass spectrometry. Most of the identified metabolites, including carbohydrates, amino acids, and organic acids, accumulated in the two types of grape after CA. Galactinol, raffinose, fructose, mannose, glycine, and ascorbate were continuously induced by cold in V amurensis, but not in Muscat of Hamburg. Twelve metabolites, including isoleucine, valine, proline, 2-oxoglutarate, and putrescine, increased in V amurensis during CA. More galactinol, ascorbate, 2-oxoglutarate, and putrescine, accumulated in V amurensis, but not in Muscat of Hamburg, during CA, which may be responsible for the excellent cold tolerance in V amurensis. The expression levels of the genes encoding (3-amylase (BAMY), galactinol synthase (GoIS), and raffinose synthase (RafS) were evaluated by quantitative reverse transcription-PCR. The expression BAMY (VIT_02s0012 g00170) and RafS (VIT_05s0077 g00840) were primarily responsible for the accumulation of maltose and raffinose, respectively. The accumulation of galactinol was attributed to different members of GoIS in the two grapes. In conclusion, these results show the inherent differences in metabolites between V amurensis and V. vinifera under CA. |
| Subject Area | Plant Sciences ; Genetics & Heredity ; Horticulture |
| DOI | 10.1038/s41438-018-0083-5 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | FREEZING TOLERANCE ; ARABIDOPSIS-THALIANA ; TRANSCRIPTION FACTOR ; INTEGRATED ANALYSIS ; GAS-CHROMATOGRAPHY ; ABIOTIC STRESS ; GENES ; SALT ; ACCESSIONS ; RESPONSES |
| WOS Research Area | Plant Sciences ; Genetics & Heredity ; Agriculture |
| WOS ID | WOS:000454602600008 |
| Publisher | NATURE PUBLISHING GROUP |
| Subtype | Article |
| Publication Place | LONDON |
| Funding Organization | Youth Innovation Promotion Association of CAS [2015281] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471857, 31672132] ; Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia [NXNYYZ201502] |
| Corresponding Author Email | xinhaiping251@hotmail.com ; shhli@ibcas.ac.cn |
| OA | Green Published, gold |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/19571 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 5.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China 6.Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Guangzhou, Guangdong, Peoples R China |
| Recommended Citation GB/T 7714 | Chai, Fengmei,Liu, Wenwen,Xiang, Yue,et al. Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation[J]. HORTICULTURE RESEARCH,2019,6. |
| APA | Chai, Fengmei.,Liu, Wenwen.,Xiang, Yue.,Meng, Xianbin.,Sun, Xiaoming.,...&Li, Shaohua.(2019).Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation.HORTICULTURE RESEARCH,6. |
| MLA | Chai, Fengmei,et al."Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation".HORTICULTURE RESEARCH 6(2019). |
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| Chai-2019-Comparativ（1776KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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