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| Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility | |
Chen, Shuangyan; Jia, Junting1; Cheng, Liqin; Zhao, Pincang2; Qi, Dongmei; Yang, Weiguang; Liu, Hui; Dong, Xiaobing; Li, Xiaoxia; Liu, Gongshe
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| 2019 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 卷号 | 20期号:9 |
| 摘要 | Sheepgrass (Leymus chinensis (Trin.) Tzvel.) is an economically and ecologically important forage in the grass family. Self-incompatibility (SI) limits its seed production due to the low seed-setting rate after self-pollination. However, investigations into the molecular mechanisms of sheepgrass SI are lacking. Therefore, microscopic observation of pollen germination and pollen tube growth, as well as transcriptomic analyses of pistils after self- and cross-pollination, were performed. The results indicated that pollen tube growth was rapidly inhibited from 10 to 30 min after self-pollination and subsequently stopped but preceded normally after cross-pollination. Time course comparative transcriptomics revealed different transcriptome dynamics between self- and cross-pollination. A pool of SI-related signaling genes and pathways was generated, including genes related to calcium (Ca2+) signaling, protein phosphorylation, plant hormone, reactive oxygen species (ROS), nitric oxide (NO), cytoskeleton, and programmed cell death (PCD). A putative SI response molecular model in sheepgrass was presented. The model shows that SI may trigger a comprehensive calcium- and phytohormone-dominated signaling cascade and activate PCD, which may explain the rapid inhibition of self-pollen tube growth as observed by cytological analyses. These results provided new insight into the molecular mechanisms of sheepgrass (grass family) SI. |
| 关键词 | sheepgrass (Leymus chinensis (Trin ) self-pollination cross-pollination self-incompatibility transcriptome molecular mechanisms |
| 学科领域 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| DOI | 10.3390/ijms20092356 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | PROGRAMMED CELL-DEATH ; POLLEN-TUBE GROWTH ; NITRIC-OXIDE ; S-LOCUS ; PROTEIN ; KINASE ; GENE ; DETERMINANT ; RNASE ; IDENTIFICATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000469753500303 |
| 出版者 | MDPI |
| 文献子类 | Article |
| 出版地 | BASEL |
| EISSN | 1422-0067 |
| 资助机构 | National Key Research and Development Program of China [2018YFD1001000, 2016YFC0500700] ; Science and Technology Major Project of Inner Mongolia Autonomous Region of China ; Kulun Banner Science and Technology Poverty Alleviation Project of Chinese Academy of Sciences ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB138704] |
| 作者邮箱 | sychen@ibcas.ac.cn ; jiajunting123@126.com ; lqcheng@ibcas.ac.cn ; zhaopincang@163.com ; qidm@ibcas.ac.cn ; anda580@163.com ; liuhuisunflower@126.com ; xiaobingdong1022@163.com ; lixx2013@ibcas.ac.cn ; liugs@ibcas.ac.cn |
| 作品OA属性 | Green Published, Green Submitted, gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19579 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China 3.Hebei Univ Econ & Business, Coll Management Sci & Engn, Shijiazhuang 050061, Hebei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Shuangyan,Jia, Junting,Cheng, Liqin,et al. Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(9). |
| APA | Chen, Shuangyan.,Jia, Junting.,Cheng, Liqin.,Zhao, Pincang.,Qi, Dongmei.,...&Liu, Gongshe.(2019).Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(9). |
| MLA | Chen, Shuangyan,et al."Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.9(2019). |
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