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| Calmodulin-like gene MtCML40 is involved in salt tolerance by regulating MtHKTs transporters in Medicago truncatula | |
Zhang, Xiuxiu; Wang, Tianzuo; Liu, Min; Sun, Wei2; Zhang, Wen-Hao
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| 2019 | |
| 发表期刊 | ENVIRONMENTAL AND EXPERIMENTAL BOTANY
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| ISSN | 0098-8472 |
| 卷号 | 157页码:79-90 |
| 摘要 | Calcium (Ca2+) is a universal messenger mediating numerous physiological processes in responses to developmental and environmental cues in plant cells. Calmodulin (CaM) and calmodulin-like proteins (CMLs) are important plant Ca2+ sensors involved in decoding Ca2+ signatures to execute downstream physiological responses. Despite the involvements of CML proteins in the regulation of developmental processes, little is known about the function of CMLs in response to abiotic stresses in plants. To characterize CML proteins, we isolated and functionally characterized a gene encoding a CML protein from legume model plant Medicago truncatula, referred to as MtCML40. The MtCML40 belonged to subgroup VI of CML family. Expression of MtCML40 was up-regulated by salt, cold and osmotic stress as well as ABA treatment, suggesting a role of MtCML40 in abiotic stress. To test this hypothesis, we generated MtCML40 overexpressing transgenic lines in M. truncatula. Overexpression of MtCML40 rendered seed germination more sensitive to salt stress as evidenced by greater inhibition of seed germination of transgenic lines than wild-type seeds when exposed to NaCI, while seed germination of WT and transgenic lines was comparable under control conditions. In addition to seed germination, exposure to salt stress led to greater inhibition of shoot and root growth, reduction in chlorophyll and carotenoid concentrations and photosynthetic rates in the transgenic lines than WT plants, suggesting a negative regulation of salt tolerance by MtCML40. The greater accumulation of Na+ in shoots of transgenic lines may account for the greater sensitivity to salt stress. We further found that overexpression of MtCML40 resulted in down-regulation of MtHKT1;1 and MtlIKT1;2 that encoded proteins associated with removal of Na+ from shoots. Taken together, our results demonstrate that MtCML40 is involved in the regulation of salt tolerance by targeting MtHKT-dependent Na+ accumulation in M. truncoula. |
| 关键词 | Medicago truncatula Calmodulin-like proteins MtCML40 Salt stress MtHKTs Na+ accumulation |
| 学科领域 | Plant Sciences ; Environmental Sciences |
| DOI | 10.1016/j.envexpbot.2018.09.022 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | POTENTIAL CALCIUM SENSORS ; ARABIDOPSIS-THALIANA ; PLASMA-MEMBRANE ; ABSCISIC-ACID ; NA+/H+ EXCHANGER ; ABIOTIC STRESS ; EXPRESSION ; PROTEIN ; ATHKT1 ; RESPONSES |
| WOS研究方向 | Plant Sciences ; Environmental Sciences & Ecology |
| WOS记录号 | WOS:000454465500008 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 文献子类 | Article |
| 出版地 | OXFORD |
| EISSN | 1873-7307 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31301832, 31470446] |
| 作者邮箱 | whzhang@ibcas.ac.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19643 |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Northeast Normal Univ, Inst Grassland Sci, Key Lab Vegetat Ecol, Minist Educ, Changchun, Jilin, Peoples R China 4.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Global Change Biol, Beijing, Peoples R China 5.Chinese Acad Sci, Inner Mongolia Res Ctr Prataculture, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiuxiu,Wang, Tianzuo,Liu, Min,et al. Calmodulin-like gene MtCML40 is involved in salt tolerance by regulating MtHKTs transporters in Medicago truncatula[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2019,157:79-90. |
| APA | Zhang, Xiuxiu,Wang, Tianzuo,Liu, Min,Sun, Wei,&Zhang, Wen-Hao.(2019).Calmodulin-like gene MtCML40 is involved in salt tolerance by regulating MtHKTs transporters in Medicago truncatula.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,157,79-90. |
| MLA | Zhang, Xiuxiu,et al."Calmodulin-like gene MtCML40 is involved in salt tolerance by regulating MtHKTs transporters in Medicago truncatula".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 157(2019):79-90. |
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