IB-CAS  > 中科院光生物学重点实验室
An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize
Jiang, Jingjing1; Chai, Xin1; Manavski, Nikolay2,6; Williams-Carrier, Rosalind; He, Baoye; Brachmann, Aandreas4; Ji, Daili; Ouyang, Min; Liu, Yini; Barkan, Alice3; Meurer, Joerg2; Zhang, Lixin5; Chi, Wei1
2019
发表期刊PLANT CELL
ISSN1040-4651
卷号31期号:6页码:1308-1327
摘要A key characteristic of chloroplast gene expression is the predominance of posttranscriptional control via numerous nucleus-encoded RNA binding factors. Here, we explored the essential roles of the S1-domain-containing protein photosynthetic electron transfer B (petB)/ petD Stabilizing Factor (BSF) in the stabilization and translation of chloroplast mRNAs. BSF binds to the intergenic region of petB-petD, thereby stabilizing 39 processed petB transcripts and stimulating petD translation. BSF also binds to the 59 untranslated region of petA and activates its translation. BSF displayed nucleic-acid-melting activity in vitro, and its absence induces structural changes to target RNAs in vivo, suggesting that BSF functions as an RNA chaperone to remodel RNA structure. BSF physically interacts with the pentatricopeptide repeat protein Chloroplast RNA Processing 1 (AtCRP1) and the ribosomal release factor-like protein Peptide chain Release Factor 3 (PrfB3), whose established RNA ligands overlap with those of BSF. In addition, PrfB3 stimulated the RNA binding ability of BSF in vitro. We propose that BSF and PrfB3 cooperatively reduce the formation of secondary RNA structures within target mRNAs and facilitate AtCRP1 binding. The translation activation function of BSF for petD is conserved in Arabidopsis (Arabidopsis thaliana) and maize (Zea mays), but that for petA operates specifically in Arabidopsis. Our study sheds light on the mechanisms by which RNA binding proteins cooperatively regulate mRNA stability and translation in chloroplasts.
学科领域Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI10.1105/tpc.18.00946
收录类别SCI
语种英语
WOS关键词PENTATRICOPEPTIDE REPEAT PROTEIN ; ESCHERICHIA-COLI CSPE ; ACID MELTING ACTIVITY ; BINDING DOMAIN ; PHOTOSYSTEM-II ; TRANSCRIPTION ANTITERMINATION ; RIBOSOMAL-SUBUNITS ; SPLICING FACTOR ; NUCLEAR GENES ; COMPLEX
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS记录号WOS:000471166900025
出版者OXFORD UNIV PRESS INC
文献子类Article
出版地CARY
EISSN1532-298X
资助机构Chinese Academy of Sciences (CAS) Strategic Priority Research Program [XDB17030100] ; Ministry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology) Major State Basic Research Development Program [2015CB150101] ; German Science Foundation (DFG)German Research Foundation (DFG) [TRR175 A03] ; US National Science FoundationNational Science Foundation (NSF) [IOS-1339130]
作者邮箱chiweimr@ibcas.ac.cn
作品OA属性Bronze, Green Published
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/19668
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Ludwig Maximilians Univ Munchen, Biozentrum, Plant Mol Biol, D-82152 Planegg Martinsried, Germany
4.Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA
5.Ludwig Maximilians Univ Munchen, Genet, Fac Biol, D-82152 Planegg Martinsried, Germany
6.Henan Univ, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Sch Life Sci, Kaifeng 475004, Peoples R China
7.CNRS, Inst Biol Mol 5 Plantes, 12 Rue Gen Zimmer, F-67084 Strasbourg, France
推荐引用方式
GB/T 7714
Jiang, Jingjing,Chai, Xin,Manavski, Nikolay,et al. An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize[J]. PLANT CELL,2019,31(6):1308-1327.
APA Jiang, Jingjing.,Chai, Xin.,Manavski, Nikolay.,Williams-Carrier, Rosalind.,He, Baoye.,...&Chi, Wei.(2019).An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize.PLANT CELL,31(6),1308-1327.
MLA Jiang, Jingjing,et al."An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize".PLANT CELL 31.6(2019):1308-1327.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Jiang-2019-An RNA Ch(2164KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jiang, Jingjing]的文章
[Chai, Xin]的文章
[Manavski, Nikolay]的文章
百度学术
百度学术中相似的文章
[Jiang, Jingjing]的文章
[Chai, Xin]的文章
[Manavski, Nikolay]的文章
必应学术
必应学术中相似的文章
[Jiang, Jingjing]的文章
[Chai, Xin]的文章
[Manavski, Nikolay]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Jiang-2019-An RNA Chaperone-Like Protein Plays.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。