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An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize
Jiang, Jingjing1; Chai, Xin1; Manavski, Nikolay2,6; Williams-Carrier, Rosalind; He, Baoye; Brachmann, Aandreas4; Ji, Daili; Ouyang, Min; Liu, Yini; Barkan, Alice3; Meurer, Joerg2; Zhang, Lixin5; Chi, Wei1
2019
Source PublicationPLANT CELL
ISSN1040-4651
Volume31Issue:6Pages:1308-1327
AbstractA key characteristic of chloroplast gene expression is the predominance of posttranscriptional control via numerous nucleus-encoded RNA binding factors. Here, we explored the essential roles of the S1-domain-containing protein photosynthetic electron transfer B (petB)/ petD Stabilizing Factor (BSF) in the stabilization and translation of chloroplast mRNAs. BSF binds to the intergenic region of petB-petD, thereby stabilizing 39 processed petB transcripts and stimulating petD translation. BSF also binds to the 59 untranslated region of petA and activates its translation. BSF displayed nucleic-acid-melting activity in vitro, and its absence induces structural changes to target RNAs in vivo, suggesting that BSF functions as an RNA chaperone to remodel RNA structure. BSF physically interacts with the pentatricopeptide repeat protein Chloroplast RNA Processing 1 (AtCRP1) and the ribosomal release factor-like protein Peptide chain Release Factor 3 (PrfB3), whose established RNA ligands overlap with those of BSF. In addition, PrfB3 stimulated the RNA binding ability of BSF in vitro. We propose that BSF and PrfB3 cooperatively reduce the formation of secondary RNA structures within target mRNAs and facilitate AtCRP1 binding. The translation activation function of BSF for petD is conserved in Arabidopsis (Arabidopsis thaliana) and maize (Zea mays), but that for petA operates specifically in Arabidopsis. Our study sheds light on the mechanisms by which RNA binding proteins cooperatively regulate mRNA stability and translation in chloroplasts.
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI10.1105/tpc.18.00946
Indexed BySCI
Language英语
WOS KeywordPENTATRICOPEPTIDE REPEAT PROTEIN ; ESCHERICHIA-COLI CSPE ; ACID MELTING ACTIVITY ; BINDING DOMAIN ; PHOTOSYSTEM-II ; TRANSCRIPTION ANTITERMINATION ; RIBOSOMAL-SUBUNITS ; SPLICING FACTOR ; NUCLEAR GENES ; COMPLEX
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS IDWOS:000471166900025
PublisherOXFORD UNIV PRESS INC
SubtypeArticle
Publication PlaceCARY
EISSN1532-298X
Funding OrganizationChinese Academy of Sciences (CAS) Strategic Priority Research Program [XDB17030100] ; Ministry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology) Major State Basic Research Development Program [2015CB150101] ; German Science Foundation (DFG)German Research Foundation (DFG) [TRR175 A03] ; US National Science FoundationNational Science Foundation (NSF) [IOS-1339130]
Corresponding Author Emailchiweimr@ibcas.ac.cn
OABronze, Green Published
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19668
Collection中科院光生物学重点实验室
Affiliation1.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Ludwig Maximilians Univ Munchen, Biozentrum, Plant Mol Biol, D-82152 Planegg Martinsried, Germany
4.Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA
5.Ludwig Maximilians Univ Munchen, Genet, Fac Biol, D-82152 Planegg Martinsried, Germany
6.Henan Univ, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Sch Life Sci, Kaifeng 475004, Peoples R China
7.CNRS, Inst Biol Mol 5 Plantes, 12 Rue Gen Zimmer, F-67084 Strasbourg, France
Recommended Citation
GB/T 7714
Jiang, Jingjing,Chai, Xin,Manavski, Nikolay,et al. An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize[J]. PLANT CELL,2019,31(6):1308-1327.
APA Jiang, Jingjing.,Chai, Xin.,Manavski, Nikolay.,Williams-Carrier, Rosalind.,He, Baoye.,...&Chi, Wei.(2019).An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize.PLANT CELL,31(6),1308-1327.
MLA Jiang, Jingjing,et al."An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize".PLANT CELL 31.6(2019):1308-1327.
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