Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells

doi:10.3390/ijms20112753

	Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells
	Li, Xin 1; Diao, Min 2,3; Zhang, Yanan 4; Chen, Guanlin 5; Huang, Shanjin 2; Chen, Naizhi
	2019
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	20 期号:11
摘要	The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin filaments in guard cells will enhance our understanding in this regard. Here, we initially developed a program called guard cell microfilament analyzer' (GCMA) that enables the selection of individual actin filaments and analysis of their orientations semiautomatically in guard cells. We next traced the dynamics of individual actin filaments and performed careful quantification in open and closed stomata. We found that de novo nucleation of actin filaments occurs at both dorsal and ventral sides of guard cells from open and closed stomata. Interestingly, most of the nucleated actin filaments elongate radially and longitudinally in open and closed stomata, respectively. Strikingly, radial filaments tend to form bundles whereas longitudinal filaments tend to be removed by severing and depolymerization in open stomata. By contrast, longitudinal filaments tend to form bundles that are severed less frequently in closed stomata. These observations provide insights into the formation and maintenance of distinct actin arrays in guard cells in stomata of different apertures.
关键词	guard cell microfilament angle (GCMA) individual actin filaments different orientations actin dynamics technical advance Arabidopsis
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms20112753
收录类别	SCI
语种	英语
WOS关键词	STOMATAL MOVEMENT ; MICROTUBULE ORGANIZATION ; ABSCISIC-ACID ; REORGANIZATION ; TURNOVER ; CLOSURE ; FUSION ; GROWTH ; ARRAY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000472634100147
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31671398]
作者邮箱	lixinqiu@126.com ; diaomin@shanghaitech.edu.cn ; nanknight@foxmail.com ; hackinfer@gmail.com ; sjhuang@tsinghua.edu.cn ; chennaizhi@ibcas.ac.cn
作品OA属性	Green Published, gold, Green Submitted
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19696
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 4.Shanghai Tech Univ, iHuman Inst, Shanghai 201210, Peoples R China 5.OLYMPUS CHINA CO LTD, Beijing 100027, Peoples R China 6.Baidu Online Network Technol Beijing CO LTD, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Li, Xin,Diao, Min,Zhang, Yanan,et al. Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(11).
APA	Li, Xin,Diao, Min,Zhang, Yanan,Chen, Guanlin,Huang, Shanjin,&Chen, Naizhi.(2019).Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(11).
MLA	Li, Xin,et al."Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.11(2019).

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