NADPH Oxidase Is Crucial for the Cellular Redox Homeostasis in Fungal Pathogen Botrytis cinerea

doi:10.1094/MPMI-05-19-0124-R

	NADPH Oxidase Is Crucial for the Cellular Redox Homeostasis in Fungal Pathogen Botrytis cinerea
	Li, Hua 1; Tian, Shiping1,2 ; Qin, Guozheng2
	2019
发表期刊	MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN	0894-0282
卷号	32 期号:11 页码:1508-1516
摘要	During interactions, both plants and pathogens produce reactive oxygen species (ROS). Plants generate ROS for defense induction, while pathogens synthesize ROS for growth, sporulation, and virulence. NADPH oxidase (NOX) complex in the plasma membrane represents a main protein complex for ROS production in pathogens. Although NOX plays a crucial role in pathogenicity of pathogens, the underlying molecular mechanisms of NOX, especially the proteins regulated by NOX, remain largely unknown. Here, we applied an iodoacetyl tandem mass tag-based redox proteomic assay to investigate the protein redox dynamics in deletion mutant of bcnoxR, which encodes a regulatory subunit of NOX in the fungal pathogen Botrytis cinerea. In total, 214 unique peptidyl cysteine (Cys) thiols from 168 proteins were identified and quantified in both the wild type and Delta bcnoxR mutant. The Cys thiols in the Delta bcnoxR mutant were generally more oxidized than those in the wild type, suggesting that BcNoxR is essential for maintaining the equilibrium of the redox state in B. cinerea. Site-specific thiol oxidation analysis indicated that 142 peptides containing the oxidized thiols changed abundance significantly in the Delta bcnoxR mutant. Proteins containing these differential peptides are classified into various functional categories. Functional analysis revealed that one of these proteins, 6-phosphate dehydrogenase, played roles in oxidative stress response and pathogenesis of B. cinerea. These results provide insight into the potential target proteins and the ROS signal transduction pathway regulated by NOX.
关键词	mechanisms of pathogenicity proteomics
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1094/MPMI-05-19-0124-R
收录类别	SCI
语种	英语
WOS关键词	REACTIVE OXYGEN ; REGULATORY SUBUNIT ; SEXUAL DEVELOPMENT ; DIFFERENTIATION ; VIRULENCE ; GLUTATHIONYLATION ; IDENTIFICATION ; TRANSCRIPTION ; HOMOLOG ; PATHWAY
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号	WOS:000494258900007
出版者	AMER PHYTOPATHOLOGICAL SOC
文献子类	Article
出版地	ST PAUL
EISSN	1943-7706
资助机构	National Key Research and Development Program [2018YFD1000200] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31172004]
作者邮箱	gzqin@ibcas.ac.cn
作品OA属性	hybrid
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19697
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Minist Agr China, Key Lab Postharvest Handing Fruits, Hangzhou 310021, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Li, Hua,Tian, Shiping,Qin, Guozheng. NADPH Oxidase Is Crucial for the Cellular Redox Homeostasis in Fungal Pathogen Botrytis cinerea[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2019,32(11):1508-1516.
APA	Li, Hua,Tian, Shiping,&Qin, Guozheng.(2019).NADPH Oxidase Is Crucial for the Cellular Redox Homeostasis in Fungal Pathogen Botrytis cinerea.MOLECULAR PLANT-MICROBE INTERACTIONS,32(11),1508-1516.
MLA	Li, Hua,et al."NADPH Oxidase Is Crucial for the Cellular Redox Homeostasis in Fungal Pathogen Botrytis cinerea".MOLECULAR PLANT-MICROBE INTERACTIONS 32.11(2019):1508-1516.

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