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OHP1, OHP2, and HCF244 Form a Transient Functional Complex with the Photosystem II Reaction Center | |
Li, Yonghong1; Liu, Bei1; Zhang, Jiao; Kong, Fanna; Zhang, Lin2; Meng, Han2; Li, Wenjing3; Rochaix, Jean-David; Li, Dan; Peng, Lianwei2 | |
2019 | |
发表期刊 | PLANT PHYSIOLOGY |
ISSN | 0032-0889 |
卷号 | 179期号:1页码:195-208 |
摘要 | The reaction center (RC) of photosystem II (PSII), which is composed of D1, D2, PsbI, and cytochrome b559 subunits, forms at an early stage of PSII biogenesis. However, it is largely unclear how these components assemble to form a functional unit. In this work, we show that synthesis of the PSII core proteins D1/D2 and formation of the PSII RC is blocked specifically in the absence of ONE-HELIX PROTEIN1 (OHP1) and OHP2 proteins in Arabidopsis (Arabidopsis thaliana), indicating that OHP1 and OHP2 are essential for the formation of the PSII RC. Mutagenesis of the chlorophyll-binding residues in OHP proteins impairs their function and/or stability, suggesting that they may function in the binding of chlorophyll in vivo. We further show that OHP1, OHP2, and HIGH CHLOROPHYLL FLUORESCENCE244 (HCF244), together with D1, D2, PsbI, and cytochrome b559, form a complex. We designated this complex the PSII RC-like complex to distinguish it from the RC subcomplex in the intact PSII complex. Our data imply that OHP1, OHP2, and HCF244 are present in this PSII RC-like complex for a limited time at an early stage of PSII de novo assembly and of PSII repair under high-light conditions. In a subsequent stage of PSII biogenesis, OHP1, OHP2, and HCF244 are released from the PSII RC-like complex and replaced by the other PSII subunits. Together with previous reports on the cyanobacterium Synechocystis, our results demonstrate that the process of PSII RC assembly is highly conserved among photosynthetic species. |
学科领域 | Plant Sciences |
DOI | 10.1104/pp.18.01231 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | LIGHT-HARVESTING COMPLEX ; PSBA MESSENGER-RNA ; CHLOROPHYLL BINDING ; AUXILIARY PROTEINS ; CRYSTAL-STRUCTURE ; D1 PROTEIN ; BIOGENESIS ; REPAIR ; STABILITY ; CHLOROPLASTS |
WOS研究方向 | Plant Sciences |
WOS记录号 | WOS:000454929100017 |
出版者 | AMER SOC PLANT BIOLOGISTS |
文献子类 | Article |
出版地 | ROCKVILLE |
EISSN | 1532-2548 |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470760] ; National Natural Science Foundation for Outstanding YouthNational Natural Science Foundation of China (NSFC) [31322007] ; Shanghai Engineering Research Center of Plant Germplasm Resources [17DZ2252700] |
作者邮箱 | penglianwei@shnu.edu.cn |
作品OA属性 | Green Published, Bronze |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19698 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China 4.Langfang Teachers Univ, Coll Life Sci, Langfang 065000, Hebei, Peoples R China 5.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland 6.Univ Geneva, Dept Plant Biol, CH-1211 Geneva, Switzerland |
推荐引用方式 GB/T 7714 | Li, Yonghong,Liu, Bei,Zhang, Jiao,et al. OHP1, OHP2, and HCF244 Form a Transient Functional Complex with the Photosystem II Reaction Center[J]. PLANT PHYSIOLOGY,2019,179(1):195-208. |
APA | Li, Yonghong.,Liu, Bei.,Zhang, Jiao.,Kong, Fanna.,Zhang, Lin.,...&Peng, Lianwei.(2019).OHP1, OHP2, and HCF244 Form a Transient Functional Complex with the Photosystem II Reaction Center.PLANT PHYSIOLOGY,179(1),195-208. |
MLA | Li, Yonghong,et al."OHP1, OHP2, and HCF244 Form a Transient Functional Complex with the Photosystem II Reaction Center".PLANT PHYSIOLOGY 179.1(2019):195-208. |
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Li-2019-OHP1, OHP2, (2073KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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