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Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica
Li, Q. X.; Wang, J.; Zheng, S.; Yang, N.; Sun, K.; He, C. Y.
2019
发表期刊BIOLOGIA PLANTARUM
ISSN0006-3134
卷号63页码:153-163
摘要Virus-induced gene silencing (VIGS) is a particularly useful tool for functional genomics. In the present study, we attempted to utilize this technology to infer the function of genes from Viola philippica using a tobacco rattle virus (TRV) construct. Firstly, the phytoene desaturase gene from V. philippica (VpPDS) was silenced, and local leaf bleaching was observed but did not exhibit systemic effects, thereby limiting utilization of TRV-mediated gene silencing in the recipient plant. However, we observed systemic gene silencing in Nicotiana benthamiana when the VpPDS sequence was used as a trigger, thereby suggesting that heterologous gene sequences could elicit gene silencing. We then investigated the role of gene PISTILLATA from V. philippica (VpPI) of the B-class MADS-box gene family, which regulates the identity and development of stamens and petals. Using the coding region of VpPI as triggers, we determined the gene silencing efficiency of the corresponding GLOBOSA paralogs in N. benthamiana (NbGLO1 and NbGLO2), and we observed stamen-to-carpel transformation and distorted corollas in N. benthamiana suggesting that VpPI functioned as the NbGLO gene. However, the 3'-untranslated region (3'UTR) of VpPI and each 3'UTR of the NbGLO genes downregulated its own corresponding gene, hardly producing floral homeotic alterations. This work provides a better understanding of gene-specific probe design for gene silencing as well as shows that heterologous sequence-triggered VIGS is an efficient way to investigate functional conservation of orthologous genes.
关键词gene-specific probe GLOBOSA phytoene desaturase PISTILLATA
学科领域Plant Sciences
DOI10.32615/bp.2019.018
收录类别SCI
语种英语
WOS关键词TOBACCO RATTLE VIRUS ; EXPRESSION ; ARABIDOPSIS ; PISTILLATA ; EVOLUTION ; VECTORS ; FLOWER ; VIGS
WOS研究方向Plant Sciences
WOS记录号WOS:000466354600020
出版者ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
文献子类Article
出版地PRAHA 6
EISSN1573-8264
资助机构NSFCNational Natural Science Foundation of China (NSFC) [31560066] ; CAS/SAFEA International Partner Program for Creative Research Teams of Systematic and Evolution Botany
作者邮箱liqiaoxia8024@163.com ; kunsun@163.com
作品OA属性gold
引用统计
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/19708
专题系统与进化植物学国家重点实验室
作者单位1.[Li, Q. X.
2.Wang, J.
3.Zheng, S.
4.Yang, N.
5.Sun, K.] Northwest Normal Univ, Life Sci Coll, Lanzhou 730070, Gansu, Peoples R China
6.[He, C. Y.] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
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GB/T 7714
Li, Q. X.,Wang, J.,Zheng, S.,et al. Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica[J]. BIOLOGIA PLANTARUM,2019,63:153-163.
APA Li, Q. X.,Wang, J.,Zheng, S.,Yang, N.,Sun, K.,&He, C. Y..(2019).Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica.BIOLOGIA PLANTARUM,63,153-163.
MLA Li, Q. X.,et al."Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica".BIOLOGIA PLANTARUM 63(2019):153-163.
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