CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber

doi:10.1093/jxb/ery336

	CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber
	Liu, Mengyu ; Zhang, Cunjia ; Duan, Lixin 2; Luan, Qianqian ; Li, Jialin ; Yang, Aigang 3; Qi, Xiaoquan1 ; Ren, Zhonghai
	2019
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	70 期号:1 页码:69-84
摘要	Spine colour is an important fruit quality trait that influences the commercial value of cucumber (Cucumis sativus). However, little is known about the metabolites and the regulatory mechanisms of their biosynthesis in black spine varieties. In this study, we determined that the pigments of black spines are flavonoids, including flavonols and proanthocyanidins (PAs). We identified CsMYB60 as the best candidate for the previously identified B (Black spine) locus. Expression levels of CsMYB60 and the key genes involved in flavonoid biosynthesis were higher in black-spine inbred lines than that in white-spine lines at different developmental stages. The insertion of a Mutator-like element (CsMULE) in the second intron of CsMYB60 decreased its expression in a white-spine line. Transient overexpression assays indicated that CsMYB60 is a key regulatory gene and Cs4CL is a key structural gene in the pigmentation of black spines. In addition, the DNA methylation level in the CsMYB60 promoter was much lower in the black-spine line compared with white-spine line. The CsMULE insert may decrease the expression level of CsMYB60, causing hindered synthesis of flavonols and PAs in cucumber fruit spines.
关键词	CsMYB60 cucumber epigenetic alteration flavonols fruit spine Mutator-like element proanthocyanidins
学科领域	Plant Sciences
DOI	10.1093/jxb/ery336
收录类别	SCI
语种	英语
WOS关键词	MUTATOR-LIKE ELEMENTS ; KAPPA-B ; TRANSPOSON ; GENE ; TRANSCRIPTION ; BIOSYNTHESIS ; MAIZE ; DOMESTICATION ; ARABIDOPSIS ; EXPRESSION
WOS研究方向	Plant Sciences
WOS记录号	WOS:000459348500008
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31672170, 31222048] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [JQ201309] ; Shandong 'Double Tops' Program [SYL2017YSTD06] ; 'Taishan Scholar' Foundation of the People's Government of Shandong Province [ts20130932]
作者邮箱	xqi@ibcas.ac.cn ; zhren@sdau.edu.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19716
专题	中科院植物分子生理学重点实验室
作者单位	1.Shandong Agr Univ, Shandong Collaborat Innovat Ctr Fruit & Vegetable, Key Lab Biol & Genet Improvement Hort Crops Huang, State Key Lab Corp Biol,Minist Agr,Coll Hort Sci, Tai An 271018, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, 20 Nanxincun, Beijing 100093, Peoples R China 3.Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Guangzhou, Guangdong, Peoples R China 4.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Liu, Mengyu,Zhang, Cunjia,Duan, Lixin,et al. CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(1):69-84.
APA	Liu, Mengyu.,Zhang, Cunjia.,Duan, Lixin.,Luan, Qianqian.,Li, Jialin.,...&Ren, Zhonghai.(2019).CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber.JOURNAL OF EXPERIMENTAL BOTANY,70(1),69-84.
MLA	Liu, Mengyu,et al."CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber".JOURNAL OF EXPERIMENTAL BOTANY 70.1(2019):69-84.

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