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Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes
Yang, Qi; Han, Xue-Min; Gu, Jin-Ke; Liu, Yan-Jing; Yang, Mao-Jun; Zeng, Qing-Yin
2019
发表期刊NEW PHYTOLOGIST
ISSN0028-646X
卷号221期号:2页码:1060-1073
摘要A common assumption in comparative genomics is that orthologous genes are functionally more similar than paralogous genes. However, the validity of this assumption needs to be assessed using robust experimental data. We conducted tissue-specific gene expression and protein function analyses of orthologous groups within the glutathione S-transferase (GST) gene family in three closely related Populus species: Populus trichocarpa, Populus euphratica and Populus yatungensis. This study identified 21 GST orthologous groups in the three Populus species. Although the sequences of the GST orthologous groups were highly conserved, the divergence in enzymatic functions was prevalent. Through site-directed mutagenesis of orthologous proteins, this study revealed that nonsynonymous substitutions at key amino acid sites played an important role in the divergence of enzymatic functions. In particular, a single amino acid mutation (Arg39 -> Trp39) contributed to P. euphratica PeGSTU30 possessing high enzymatic activity via increasing the hydrophobicity of the active cavity. This study provided experimental evidence showing that orthologues belonging to the gene family have functional divergences. The nonsynonymous substitutions at a few amino acid sites resulted in functional divergence of the orthologous genes. Our findings provide new insights into the evolution of orthologous genes in closely related species.
关键词functional divergence glutathione S-transferase (GST) orthologous gene Populus protein function
学科领域Plant Sciences
DOI10.1111/nph.15430
收录类别SCI
语种英语
WOS关键词GLUTATHIONE-S-TRANSFERASE ; SUPERGENE FAMILY ; DUPLICATE GENES ; EVOLUTIONARY ; GENOME ; DIVERGENCE ; RESISTANCE ; RESIDUES
WOS研究方向Plant Sciences
WOS记录号WOS:000453883200044
出版者WILEY
文献子类Article
出版地HOBOKEN
EISSN1469-8137
资助机构National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [31425006] ; Chinese Academy of Forestry [CAFYBB2018ZX001]
作者邮箱qingyin.zeng@ibcas.ac.cn
作品OA属性Bronze
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/19751
专题系统与进化植物学国家重点实验室
作者单位1.[Yang, Qi
2.Liu, Yan-Jing
3.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China
4.Han, Xue-Min
5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.[Gu, Jin-Ke
8.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
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GB/T 7714
Yang, Qi,Han, Xue-Min,Gu, Jin-Ke,et al. Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes[J]. NEW PHYTOLOGIST,2019,221(2):1060-1073.
APA Yang, Qi,Han, Xue-Min,Gu, Jin-Ke,Liu, Yan-Jing,Yang, Mao-Jun,&Zeng, Qing-Yin.(2019).Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes.NEW PHYTOLOGIST,221(2),1060-1073.
MLA Yang, Qi,et al."Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes".NEW PHYTOLOGIST 221.2(2019):1060-1073.
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