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Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes | |
Yang, Qi; Han, Xue-Min; Gu, Jin-Ke; Liu, Yan-Jing; Yang, Mao-Jun; Zeng, Qing-Yin | |
2019 | |
发表期刊 | NEW PHYTOLOGIST |
ISSN | 0028-646X |
卷号 | 221期号:2页码:1060-1073 |
摘要 | A common assumption in comparative genomics is that orthologous genes are functionally more similar than paralogous genes. However, the validity of this assumption needs to be assessed using robust experimental data. We conducted tissue-specific gene expression and protein function analyses of orthologous groups within the glutathione S-transferase (GST) gene family in three closely related Populus species: Populus trichocarpa, Populus euphratica and Populus yatungensis. This study identified 21 GST orthologous groups in the three Populus species. Although the sequences of the GST orthologous groups were highly conserved, the divergence in enzymatic functions was prevalent. Through site-directed mutagenesis of orthologous proteins, this study revealed that nonsynonymous substitutions at key amino acid sites played an important role in the divergence of enzymatic functions. In particular, a single amino acid mutation (Arg39 -> Trp39) contributed to P. euphratica PeGSTU30 possessing high enzymatic activity via increasing the hydrophobicity of the active cavity. This study provided experimental evidence showing that orthologues belonging to the gene family have functional divergences. The nonsynonymous substitutions at a few amino acid sites resulted in functional divergence of the orthologous genes. Our findings provide new insights into the evolution of orthologous genes in closely related species. |
关键词 | functional divergence glutathione S-transferase (GST) orthologous gene Populus protein function |
学科领域 | Plant Sciences |
DOI | 10.1111/nph.15430 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | GLUTATHIONE-S-TRANSFERASE ; SUPERGENE FAMILY ; DUPLICATE GENES ; EVOLUTIONARY ; GENOME ; DIVERGENCE ; RESISTANCE ; RESIDUES |
WOS研究方向 | Plant Sciences |
WOS记录号 | WOS:000453883200044 |
出版者 | WILEY |
文献子类 | Article |
出版地 | HOBOKEN |
EISSN | 1469-8137 |
资助机构 | National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [31425006] ; Chinese Academy of Forestry [CAFYBB2018ZX001] |
作者邮箱 | qingyin.zeng@ibcas.ac.cn |
作品OA属性 | Bronze |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19751 |
专题 | 系统与进化植物学国家重点实验室 |
作者单位 | 1.[Yang, Qi 2.Liu, Yan-Jing 3.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 4.Han, Xue-Min 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.[Gu, Jin-Ke 8.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Qi,Han, Xue-Min,Gu, Jin-Ke,et al. Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes[J]. NEW PHYTOLOGIST,2019,221(2):1060-1073. |
APA | Yang, Qi,Han, Xue-Min,Gu, Jin-Ke,Liu, Yan-Jing,Yang, Mao-Jun,&Zeng, Qing-Yin.(2019).Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes.NEW PHYTOLOGIST,221(2),1060-1073. |
MLA | Yang, Qi,et al."Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes".NEW PHYTOLOGIST 221.2(2019):1060-1073. |
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nph.154301.pdf(2710KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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