Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus

doi:10.1111/ppl.12945

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	Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus
	Sipka, Gabor ; Mueller, Pavel 1; Brettel, Klaus 1; Magyar, Melinda ; Kovacs, Laszlo ; Zhu, Qingjun ; Xiao, Yanan ; Han, Guangye ; Lambrev, Petar H.; Shen, Jian-Ren; Garab, Gyozo 4
	2019
发表期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
卷号	166 期号:1 页码:22-32
摘要	Recent chlorophyll-a fluorescence yield measurements, using single-turnover saturating flashes (STSFs), have revealed the involvement of a rate-limiting step in the reactions following the charge separation induced by the first flash. As also shown here, in diuron-inhibited PSII core complexes isolated from Thermosynechococcus vulcanus the fluorescence maximum could only be reached by a train of STSFs. In order to elucidate the origin of the fluorescence yield increments in STSF series, we performed transient absorption measurements at 819nm, reflecting the photooxidation and re-reduction kinetics of the primary electron donor P680. Upon single flash excitation of the dark-adapted sample, the decay kinetics could be described with lifetimes of 17ns (approximate to 50%) and 167ns (approximate to 30%), and a longer-lived component (approximate to 20%). This kinetics are attributed to re-reduction of P680(center dot+) by the donor side of PSII. In contrast, upon second-flash (with t between 5s and 100ms) or repetitive excitation, the 819nm absorption changes decayed with lifetimes of about 2ns (approximate to 60%) and 10ns (approximate to 30%), attributed to recombination of the primary radical pair P680(center dot+)Pheo(center dot-), and a small longer-lived component (approximate to 10%). These data confirm that only the first STSF is capable of generating stable charge separation-leading to the reduction of Q(A); and thus, the fluorescence yield increments elicited by the consecutive flashes must have a different physical origin. Our double-flash experiments indicate that the rate-limiting steps, detected by chlorophyll-a fluorescence, are not correlated with the turnover of P680.
学科领域	Plant Sciences
DOI	10.1111/ppl.12945
收录类别	SCI
语种	英语
WOS关键词	PRIMARY ELECTRON-DONOR ; PRIMARY-CHARGE SEPARATION ; REDUCTION KINETICS ; ABSORPTION-SPECTROSCOPY ; RECOMBINATION PATHWAYS ; ABSORBENCY CHANGES ; P-680 ; PHEOPHYTIN ; EXCITATION ; PARTICLES
WOS研究方向	Plant Sciences
WOS记录号	WOS:000466108300004
出版者	WILEY
Special Issue	SI
文献子类	Article; Proceedings Paper
出版地	HOBOKEN
EISSN	1399-3054
资助机构	National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [NKFIH OTKA KH 124985, K 128679, TET_15-1-2016-0144, NKFIH OTKA NN 124904, GINOP-2.3.2-15-2016-00001, OTKA PD 121225] ; STSM Grant from the EBSA ; French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INBS-05] ; National Key R&D Program of China [2017YFA0503700] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDB17000000] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Lendulet Program of the Hungarian Academy of Sciences [LP2014/19]
作者邮箱	sipka.gabor@brc.mta.hu ; garab.gyozo@brc.mta.hu
作品OA属性	Green Published
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19768
专题	中科院光生物学重点实验室
作者单位	1.Hungarian Acad Sci, Biol Res Ctr, Inst Plant Biol, Lab Photosynthet Membranes, Szeged, Hungary 2.Univ Paris Saclay, Univ Paris Sud, Inst Integrat Biol Cell I2BC, CEA,CNRS, F-91198 Gif Sur Yvette, France 3.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Beijing, Peoples R China 4.Okayama Univ, Photosynth Res Ctr, Okayama, Japan 5.Univ Ostrava, Fac Sci, Ostrava, Czech Republic
推荐引用方式 GB/T 7714	Sipka, Gabor,Mueller, Pavel,Brettel, Klaus,et al. Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus[J]. PHYSIOLOGIA PLANTARUM,2019,166(1):22-32.
APA	Sipka, Gabor.,Mueller, Pavel.,Brettel, Klaus.,Magyar, Melinda.,Kovacs, Laszlo.,...&Garab, Gyozo.(2019).Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus.PHYSIOLOGIA PLANTARUM,166(1),22-32.
MLA	Sipka, Gabor,et al."Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus".PHYSIOLOGIA PLANTARUM 166.1(2019):22-32.

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