DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae

doi:10.1002/ece3.4886

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	DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae
	Ren, Feng-Ming ; Wang, Ying-Wei ; Xu, Zhi-Chao ; Li, Ying ; Xin, Tian-Yi ; Zhou, Jian-Guo ; Qi, Yao-Dong ; Wei, Xue-Ping ; Yao, Hui ; Song, Jing-Yuan
	2019
发表期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
卷号	9 期号:4 页码:1934-1945
摘要	The genus Corydalis is recognized as one of the most taxonomically challenging plant taxa. It is mainly distributed in the Himalaya-Hengduan Mountains, a global biodiversity hotspot. To date, no effective solution for species discrimination and taxonomic assignment in Corydalis has been developed. In this study, five nuclear and chloroplast DNA regions, ITS, ITS2, matK, rbcL, and psbA-trnH, were preliminarily assessed based on their ability to discriminate Corydalis to eliminate inefficient regions, and the three regions showing good performance (ITS, ITS2 and matK) were then evaluated in 131 samples representing 28 species of 11 sections of four subgenera in Corydalis using three analytical methods (NJ, ML, MP tree; K2P-distance and BLAST). The results showed that the various approaches exhibit different species identification power and that BLAST shows the best performance among the tested approaches. A comparison of different barcodes indicated that among the single barcodes, ITS (65.2%) exhibited the highest identification success rate and that the combination of ITS+matK (69.6%) provided the highest species resolution among all single barcodes and their combinations. Three Pharmacopoeia-recorded medicinal plants and their materia medica were identified successfully based on the ITS and ITS2 regions. In the phylogenetic analysis, the sections Thalictrifoliae, Sophorocapnos, Racemosae, Aulacostigma, and Corydalis formed well-supported separate lineages. We thus hypothesize that the five sections should be classified as an independent subgenus and that the genus should be divided into three subgenera. In this study, DNA barcoding provided relatively high species discrimination power, indicating that it can be used for species discrimination in this taxonomically complicated genus and as a potential tool for the authentication of materia medica belonging to Corydalis.
关键词	biodiversity hotspot Corydalis DNA barcoding matK species identification the internal transcribed spacer
学科领域	Ecology ; Evolutionary Biology
DOI	10.1002/ece3.4886
收录类别	SCI
语种	英语
WOS关键词	IDENTIFICATION ; BIODIVERSITY ; AUTHENTICATION ; DIVERSITY ; RHIZOMA ; UTILITY ; L.
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
WOS记录号	WOS:000461114900031
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
资助机构	Basic scientific research project of Chongqing-18 [2018CSCT-JXJL-JBKY-0034] ; CAMS Innovation Fund for Medical Sciences (CIFMS) [2017-I2M-1-009] ; Science and technology project of traditional Chinese medicine in Chongqing [ZY201702143] ; Basic scientific research project of Chongqing-16 [2016CSTC -JBKY01308]
作者邮箱	jysong@implad.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19780
专题	植物园
作者单位	1.[Ren, Feng-Ming 2.Xu, Zhi-Chao 3.Li, Ying 4.Xin, Tian-Yi 5.Zhou, Jian-Guo 6.Qi, Yao-Dong 7.Wei, Xue-Ping 8.Yao, Hui 9.Chinese Acad Med Sci, Inst Med Plant Dev, Peking Union Med Coll,Key Lab Chinese Med Resourc, State Adm Tradit Chinese Med Peoples Republ China, Beijing, Peoples R China 10.Chinese Med Breeding & Evaluat Engn Technol Res C, Characterist Biol Resources Sichuan & Chongqing C, Chongqing Inst Med Plant Cultivat Res & Utilizat, Chongqing, Peoples R China 11.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ren, Feng-Ming,Wang, Ying-Wei,Xu, Zhi-Chao,et al. DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae[J]. ECOLOGY AND EVOLUTION,2019,9(4):1934-1945.
APA	Ren, Feng-Ming.,Wang, Ying-Wei.,Xu, Zhi-Chao.,Li, Ying.,Xin, Tian-Yi.,...&Song, Jing-Yuan.(2019).DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae.ECOLOGY AND EVOLUTION,9(4),1934-1945.
MLA	Ren, Feng-Ming,et al."DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae".ECOLOGY AND EVOLUTION 9.4(2019):1934-1945.

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