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| Comparative physiological, metabolomic, and transcriptomic analyses reveal developmental stage-dependent effects of cluster bagging on phenolic metabolism in Cabernet Sauvignon grape berries | |
| Sun, Run-Ze; Cheng, Guo4; Li, Qiang5; Zhu, Yan-Rong; Zhang, Xue7; Wang, Yu; He, Yan-Nan; Li, Si-Yu; He, Lei; Chen, Wu3; Pan, Qiu-Hong; Duan, Chang-Qing; Wang, Jun1 | |
| 2019 | |
| Source Publication | BMC PLANT BIOLOGY
 |
| ISSN | 1471-2229 |
| Volume | 19Issue:1 |
| Abstract | Background: Light conditions significantly influence grape berry ripening and the accumulation of phenolic compounds, but the underlying molecular basis remains partially understood. Here, we applied integrated transcriptomics and pathway-level metabolomics analyses to investigate the effect of cluster bagging during various developmental stages on phenolic metabolism in Cabernet Sauvignon grapes. Results: Bagging treatments had limited effects on berry quality attributes at harvest and did not consistently affect phenolic acid biosynthesis between seasons. Significantly elevated flavan-3-ol and flavonol contents were detected in re-exposed berries after bagging during early-developmental stages, while bagging after veraison markedly inhibited skin anthocyanin accumulation. Several anthocyanin derivatives and flavonol glycosides were identified as marker phenolic metabolites for distinguishing bagged and non-bagged grapes. Coordinated transcriptional changes in the light signaling components CRY2 and HY5/HYHs, transcription regulator MYBA1, and enzymes LAR, ANR, UFGT and FLS4, coincided well with light-responsive biosynthesis of the corresponding flavonoids. The activation of multiple hormone signaling pathways after both light exclusion and re-exposure treatments was inconsistent with the changes in phenolic accumulation, indicating a limited role of plant hormones in mediating light/darkness-regulated phenolic biosynthesis processes. Furthermore, gene-gene and gene-metabolite network analyses discovered that the light-responsive expression of genes encoding bHLH, MYB, WRKY, NAC, and MADS-box transcription factors, and proteins involved in genetic information processing and epigenetic regulation such as nucleosome assembly and histone acetylation, showed a high positive correlation with grape berry phenolic accumulation in response to different light regimes. Conclusions: Altogether, our findings provide novel insights into the understanding of berry phenolic biosynthesis under light/darkness and practical guidance for improving grape features. |
| Keyword | Cluster bagging Grape berry Phenolic compounds Transcriptome Vitis vinifera cv. Cabernet sauvignon VviFLS4 |
| Subject Area | Plant Sciences |
| DOI | 10.1186/s12870-019-2186-z |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | FLAVONOL SYNTHASE GENES ; ANTHOCYANIN BIOSYNTHESIS ; SUNLIGHT EXPOSURE ; PATHWAY GENES ; LEAF REMOVAL ; UV-B ; PLANT HORMONES ; ABSCISIC-ACID ; LIGHT ; EXPRESSION |
| WOS Research Area | Plant Sciences |
| WOS ID | WOS:000505241200003 |
| Publisher | BMC |
| Subtype | Article |
| Publication Place | LONDON |
| Funding Organization | China Agriculture Research System [CARS-29] ; Project of Integration of Winemaking Techniques and Product Development for Featured Chateau in Xinjiang Province [2017A01001-3] |
| Corresponding Author Email | jun_wang@cau.edu.cn |
| OA | gold, Green Published |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/19801 |
| Collection | 中科院北方资源植物重点实验室 |
| Affiliation | 1.China Agr Univ, Coll Food Sci & Nutr Engn, Ctr Viticulture & Enol, Beijing 100083, Peoples R China 2.Minist Agr & Rural Affairs, Key Lab Viticulture & Enol, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 4.CITIC Guoan Wine Co Ltd, Xinjiang 832200, Manasi, Peoples R China 5.Guangxi Acad Agr Sci, Grape & Wine Res Inst, Nanning 530007, Peoples R China 6.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 7.Nongfu Spring Co Ltd, Hangzhou 310000, Zhejiang, Peoples R China 8.Ruifeng Oseis Yantai Wine Manor Co Ltd, Yantai 264010, Peoples R China 9.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Hebei, Peoples R China |
| Recommended Citation GB/T 7714 | Sun, Run-Ze,Cheng, Guo,Li, Qiang,et al. Comparative physiological, metabolomic, and transcriptomic analyses reveal developmental stage-dependent effects of cluster bagging on phenolic metabolism in Cabernet Sauvignon grape berries[J]. BMC PLANT BIOLOGY,2019,19(1). |
| APA | Sun, Run-Ze.,Cheng, Guo.,Li, Qiang.,Zhu, Yan-Rong.,Zhang, Xue.,...&Wang, Jun.(2019).Comparative physiological, metabolomic, and transcriptomic analyses reveal developmental stage-dependent effects of cluster bagging on phenolic metabolism in Cabernet Sauvignon grape berries.BMC PLANT BIOLOGY,19(1). |
| MLA | Sun, Run-Ze,et al."Comparative physiological, metabolomic, and transcriptomic analyses reveal developmental stage-dependent effects of cluster bagging on phenolic metabolism in Cabernet Sauvignon grape berries".BMC PLANT BIOLOGY 19.1(2019). |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Sun-2019-Comparative（6748KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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