IB-CAS  > 中科院北方资源植物重点实验室
The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance
Sun, Xiaoming1; Zhang, Langlang; Wong, Darren C. J.; Wang, Yi1; Zhu, Zhenfei; Xu, Guangzhao1; Wang, Qingfeng; Li, Shaohua1; Liang, Zhenchang1; Xin, Haiping
2019
Source PublicationPLANT JOURNAL
ISSN0960-7412
Volume99Issue:5Pages:988-1002
AbstractCold stress is a major limiting factor in grape (Vitis) productivity. In this study, we characterized a cold-responsive ethylene response factor (ERF) transcription factor, VaERF092, from Amur grape (Vitis amurensis). VaERF092 expression was induced by both low temperatures and the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC), but was suppressed by treatment with the ethylene inhibitor aminoethoxyvinylglycine (AVG) under cold conditions. Ectopic expression of VaERF092 in Arabidopsis thaliana enhanced cold tolerance. Co-expression network analysis of V. vinifera genes indicated that WRKY33 might be a downstream target of VaERF092. This hypothesis was supported by the fact that VaWRKY33 was expressed temporally after VaERF092 expression and could also be induced by cold and ACC, and inhibited by AVG. Yeast one-hybrid, transient beta-glucuronidase (GUS) and dual-luciferase reporter assays provided evidence for an interaction between VaERF092 and a GCC-box element in the VaWRKY33 promoter. In addition, heterologous overexpression of VaWRKY33 in A. thaliana resulted in enhanced cold tolerance. VaERF092- and VaWRKY33 overexpressing grape calli showed lower low-temperature exothermic values than the empty vector (EV) calli, indicating enhanced tolerance to cold. Together, these results indicated that VaERF092 regulates VaWRKY33 through binding to its promoter GCC-box, leading to enhanced cold stress tolerance.
Keywordcold stress ERF transcription factor WRKY transcription factor GCC-box Vitis amurensis
Subject AreaPlant Sciences
DOI10.1111/tpj.14378
Indexed BySCI
Language英语
WOS KeywordABIOTIC STRESS TOLERANCE ; GENOME-WIDE ANALYSIS ; INDUCED CHILLING TOLERANCE ; ERF GENE FAMILY ; FREEZING TOLERANCE ; ENHANCES RESISTANCE ; VITIS-AMURENSIS ; LOW-TEMPERATURE ; OSMOTIC-STRESS ; ARABIDOPSIS
WOS Research AreaPlant Sciences
WOS IDWOS:000483586500014
PublisherWILEY
SubtypeArticle
Publication PlaceHOBOKEN
EISSN1365-313X
Corresponding Author Emailzl249@ibcas.ac.cn ; xinhaiping215@hotmail.com
OABronze, Green Published
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19807
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Plant Germ Plasm Enhancement & Specialty, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
3.Wong, Darren C. J.] Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia
Recommended Citation
GB/T 7714
Sun, Xiaoming,Zhang, Langlang,Wong, Darren C. J.,et al. The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance[J]. PLANT JOURNAL,2019,99(5):988-1002.
APA Sun, Xiaoming.,Zhang, Langlang.,Wong, Darren C. J..,Wang, Yi.,Zhu, Zhenfei.,...&Xin, Haiping.(2019).The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance.PLANT JOURNAL,99(5),988-1002.
MLA Sun, Xiaoming,et al."The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance".PLANT JOURNAL 99.5(2019):988-1002.
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