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The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance | |
Sun, Xiaoming1; Zhang, Langlang; Wong, Darren C. J.; Wang, Yi1; Zhu, Zhenfei; Xu, Guangzhao1; Wang, Qingfeng; Li, Shaohua1; Liang, Zhenchang1; Xin, Haiping | |
2019 | |
发表期刊 | PLANT JOURNAL |
ISSN | 0960-7412 |
卷号 | 99期号:5页码:988-1002 |
摘要 | Cold stress is a major limiting factor in grape (Vitis) productivity. In this study, we characterized a cold-responsive ethylene response factor (ERF) transcription factor, VaERF092, from Amur grape (Vitis amurensis). VaERF092 expression was induced by both low temperatures and the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC), but was suppressed by treatment with the ethylene inhibitor aminoethoxyvinylglycine (AVG) under cold conditions. Ectopic expression of VaERF092 in Arabidopsis thaliana enhanced cold tolerance. Co-expression network analysis of V. vinifera genes indicated that WRKY33 might be a downstream target of VaERF092. This hypothesis was supported by the fact that VaWRKY33 was expressed temporally after VaERF092 expression and could also be induced by cold and ACC, and inhibited by AVG. Yeast one-hybrid, transient beta-glucuronidase (GUS) and dual-luciferase reporter assays provided evidence for an interaction between VaERF092 and a GCC-box element in the VaWRKY33 promoter. In addition, heterologous overexpression of VaWRKY33 in A. thaliana resulted in enhanced cold tolerance. VaERF092- and VaWRKY33 overexpressing grape calli showed lower low-temperature exothermic values than the empty vector (EV) calli, indicating enhanced tolerance to cold. Together, these results indicated that VaERF092 regulates VaWRKY33 through binding to its promoter GCC-box, leading to enhanced cold stress tolerance. |
关键词 | cold stress ERF transcription factor WRKY transcription factor GCC-box Vitis amurensis |
学科领域 | Plant Sciences |
DOI | 10.1111/tpj.14378 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | ABIOTIC STRESS TOLERANCE ; GENOME-WIDE ANALYSIS ; INDUCED CHILLING TOLERANCE ; ERF GENE FAMILY ; FREEZING TOLERANCE ; ENHANCES RESISTANCE ; VITIS-AMURENSIS ; LOW-TEMPERATURE ; OSMOTIC-STRESS ; ARABIDOPSIS |
WOS研究方向 | Plant Sciences |
WOS记录号 | WOS:000483586500014 |
出版者 | WILEY |
文献子类 | Article |
出版地 | HOBOKEN |
EISSN | 1365-313X |
作者邮箱 | zl249@ibcas.ac.cn ; xinhaiping215@hotmail.com |
作品OA属性 | Bronze, Green Published |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19807 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germ Plasm Enhancement & Specialty, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Wong, Darren C. J.] Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia |
推荐引用方式 GB/T 7714 | Sun, Xiaoming,Zhang, Langlang,Wong, Darren C. J.,et al. The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance[J]. PLANT JOURNAL,2019,99(5):988-1002. |
APA | Sun, Xiaoming.,Zhang, Langlang.,Wong, Darren C. J..,Wang, Yi.,Zhu, Zhenfei.,...&Xin, Haiping.(2019).The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance.PLANT JOURNAL,99(5),988-1002. |
MLA | Sun, Xiaoming,et al."The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance".PLANT JOURNAL 99.5(2019):988-1002. |
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