The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance

doi:10.1111/tpj.14378

	The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance
	Sun, Xiaoming 1; Zhang, Langlang ; Wong, Darren C. J.; Wang, Yi 1; Zhu, Zhenfei ; Xu, Guangzhao 1; Wang, Qingfeng ; Li, Shaohua1 ; Liang, Zhenchang 1; Xin, Haiping
	2019
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	99 期号:5 页码:988-1002
摘要	Cold stress is a major limiting factor in grape (Vitis) productivity. In this study, we characterized a cold-responsive ethylene response factor (ERF) transcription factor, VaERF092, from Amur grape (Vitis amurensis). VaERF092 expression was induced by both low temperatures and the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC), but was suppressed by treatment with the ethylene inhibitor aminoethoxyvinylglycine (AVG) under cold conditions. Ectopic expression of VaERF092 in Arabidopsis thaliana enhanced cold tolerance. Co-expression network analysis of V. vinifera genes indicated that WRKY33 might be a downstream target of VaERF092. This hypothesis was supported by the fact that VaWRKY33 was expressed temporally after VaERF092 expression and could also be induced by cold and ACC, and inhibited by AVG. Yeast one-hybrid, transient beta-glucuronidase (GUS) and dual-luciferase reporter assays provided evidence for an interaction between VaERF092 and a GCC-box element in the VaWRKY33 promoter. In addition, heterologous overexpression of VaWRKY33 in A. thaliana resulted in enhanced cold tolerance. VaERF092- and VaWRKY33 overexpressing grape calli showed lower low-temperature exothermic values than the empty vector (EV) calli, indicating enhanced tolerance to cold. Together, these results indicated that VaERF092 regulates VaWRKY33 through binding to its promoter GCC-box, leading to enhanced cold stress tolerance.
关键词	cold stress ERF transcription factor WRKY transcription factor GCC-box Vitis amurensis
学科领域	Plant Sciences
DOI	10.1111/tpj.14378
收录类别	SCI
语种	英语
WOS关键词	ABIOTIC STRESS TOLERANCE ; GENOME-WIDE ANALYSIS ; INDUCED CHILLING TOLERANCE ; ERF GENE FAMILY ; FREEZING TOLERANCE ; ENHANCES RESISTANCE ; VITIS-AMURENSIS ; LOW-TEMPERATURE ; OSMOTIC-STRESS ; ARABIDOPSIS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000483586500014
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
作者邮箱	zl249@ibcas.ac.cn ; xinhaiping215@hotmail.com
作品OA属性	Bronze, Green Published
引用统计	被引频次：67[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19807
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Germ Plasm Enhancement & Specialty, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Wong, Darren C. J.] Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia
推荐引用方式 GB/T 7714	Sun, Xiaoming,Zhang, Langlang,Wong, Darren C. J.,et al. The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance[J]. PLANT JOURNAL,2019,99(5):988-1002.
APA	Sun, Xiaoming.,Zhang, Langlang.,Wong, Darren C. J..,Wang, Yi.,Zhu, Zhenfei.,...&Xin, Haiping.(2019).The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance.PLANT JOURNAL,99(5),988-1002.
MLA	Sun, Xiaoming,et al."The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance".PLANT JOURNAL 99.5(2019):988-1002.

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