IB-CAS  > 中科院植物分子生理学重点实验室
An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon
Wu, Hongyu; Xue, Xiaodong; Qin, Caihua; Xu, Yi; Guo, Yuyu; Li, Xiang; Lv, Wei; Li, Qinxia; Mao, Chuangxue; Li, Luzhao; Zhao, Suzhen1; Qi, Xiaoquan1; An, Hailong
2019
Source PublicationPLANT PHYSIOLOGY
ISSN0032-0889
Volume180Issue:1Pages:56-65
AbstractTransposon tagging is a powerful tool that has been widely applied in several species for insertional mutagenesis in plants. Several efforts have aimed to create transfer-DNA (T-DNA) insertional mutant populations in Brachypodium distachyon, a monocot plant used as a model system to study temperate cereals, but there has been a lack of research aimed at using transposon strategies. Here, we describe the application of a maize (Zea mays) Dissociation (Ds) transposon tagging system in B. distachyon. The 35S::AcTPase cassette and Ds element were constructed within the same T-DNA and transformed into B. distachyon plants. The Ds element was readily transposed to other chromosomes or to the same chromosome under the function of Activator (Ac) transposase. Through homologous chromosome synapsis, recombination, and segregation, the Ds element separated from the Ac element. We selected stable Ds-only plants using G418 and GFP assays and analyzed 241 T-0 lines, some of which were highly efficient at producing Ds-only progeny. Through thermal asymmetric interlaced PCR, we isolated 710 independent Ds flanking sequences from Ds-only plants. Furthermore, we identified a large collection of mutants with visible developmental phenotypes via this transposon tagging system. The system is relatively simple and rapid in comparison to traditional T-DNA insertion strategies, because once efficiency lines are obtained they can be reused to generate more lines from nontransposed plants without the use of time-consuming tissue culture steps.
Subject AreaPlant Sciences
DOI10.1104/pp.18.00875
Indexed BySCI
Language英语
WOS KeywordAGROBACTERIUM-MEDIATED TRANSFORMATION ; FUNCTIONAL GENOMICS ; MODEL ; ELEMENTS ; LINES ; MUTAGENESIS ; SELECTION ; BIOLOGY ; TRAP ; AC
WOS Research AreaPlant Sciences
WOS IDWOS:000466860800013
PublisherOXFORD UNIV PRESS INC
SubtypeArticle
Publication PlaceCARY
EISSN1532-2548
Funding OrganizationMinistry of Agriculture and Rural Affairs of China [2016ZX08009-002] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2014CB138102] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570290] ; State Key Laboratory of Crop Biology [dxkt201705] ; Double Tops Project of Shandong Province
Corresponding Author Emailhlan@sdau.edu.cn
OAGreen Published, Bronze
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19825
Collection中科院植物分子生理学重点实验室
Affiliation1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Wu, Hongyu,Xue, Xiaodong,Qin, Caihua,et al. An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon[J]. PLANT PHYSIOLOGY,2019,180(1):56-65.
APA Wu, Hongyu.,Xue, Xiaodong.,Qin, Caihua.,Xu, Yi.,Guo, Yuyu.,...&An, Hailong.(2019).An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon.PLANT PHYSIOLOGY,180(1),56-65.
MLA Wu, Hongyu,et al."An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon".PLANT PHYSIOLOGY 180.1(2019):56-65.
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