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Secretion of Phospholipase D delta Functions as a Regulatory Mechanism in Plant Innate Immunity
Xing, Jingjing1; Li, Xiaojuan2,3; Wang, Xiaohua; Lv, Xueqin2,3; Wang, Li; Zhang, Liang; Zhu, Yingfang1; Shen, Qianhua4; Baluska, Frantisek5; Samaj, Jozef6; Lin, Jinxing2,3
Source PublicationPLANT CELL
AbstractPlant phospholipase Ds (PLDs), essential regulators of phospholipid signaling, function in multiple signal transduction cascades; however, the mechanisms regulating PLDs in response to pathogens remain unclear. Here, we found that Arabidopsis (Arabidopsis thaliana) PLD delta accumulated in cells at the entry sites of the barley powdery mildew fungus, Blumeria graminis f. sp hordei. Using fluorescence recovery after photobleaching and single-molecule analysis, we observed higher PLD delta density in the plasma membrane after chitin treatment; PLD delta also underwent rapid exocytosis. Fluorescence resonance energy transfer with fluorescence lifetime imaging microscopy showed that the interaction between PLD delta and the microdomain marker AtREMORIN1.3 (AtREM1.3) increased in response to chitin, indicating that exocytosis facilitates rapid, efficient sorting of PLD delta into microdomains upon pathogen stimulus. We further unveiled a trade-off between brefeldin A (BFA)-resistant and -sensitive pathways in secretion of PLD delta under diverse conditions. Upon pathogen attack, PLD delta secretion involved syntaxin-associated VAMP721/722-mediated exocytosis sensitive to BFA. Analysis of phosphatidic acid (PA), hydrogen peroxide, and jasmonic acid (JA) levels and expression of related genes indicated that the relocalization of PLD delta is crucial for its activation to produce PA and initiate reactive oxygen species and JA signaling pathways. Together, our findings revealed that the translocation of PLD delta to papillae is modulated by exocytosis, thus triggering PA-mediated signaling in plant innate immunity.
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS IDWOS:000510846000018
Publication PlaceCARY
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530084, 31622005, 31900162] ; Program of Introducing Talents of Discipline to Universities (111 project)Ministry of Education, China - 111 Project [B13007] ; European Regional Development Fund (ERDF) project Plants as a tool for sustainable global development [CZ.02.1.01/0.0/0.0/16_019/0000827]
Corresponding Author Emaillinjx@ibcas.ac.cn
OAGreen Published, Bronze
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Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
2.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, Kaifeng 457004, Peoples R China
3.Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China
4.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
5.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Mol Agrobiol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
6.Rheinische Freidrich Wilhelms Univ Bonn, Inst Cellular & Mol Bot, Dept Plant Cell Biol, D-53115 Bonn, Germany
7.Palacky Univ, Fac Sci, Ctr Region Hana Biotechnol & Agr Res, Olomouc 78301, Czech Republic
Recommended Citation
GB/T 7714
Xing, Jingjing,Li, Xiaojuan,Wang, Xiaohua,et al. Secretion of Phospholipase D delta Functions as a Regulatory Mechanism in Plant Innate Immunity[J]. PLANT CELL,2019,31(12):3015-3032.
APA Xing, Jingjing.,Li, Xiaojuan.,Wang, Xiaohua.,Lv, Xueqin.,Wang, Li.,...&Lin, Jinxing.(2019).Secretion of Phospholipase D delta Functions as a Regulatory Mechanism in Plant Innate Immunity.PLANT CELL,31(12),3015-3032.
MLA Xing, Jingjing,et al."Secretion of Phospholipase D delta Functions as a Regulatory Mechanism in Plant Innate Immunity".PLANT CELL 31.12(2019):3015-3032.
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