The Protein Modifications of O-GlcNAcylation and Phosphorylation Mediate Vernalization Response for Flowering in Winter Wheat

doi:10.1104/pp.19.00081

	The Protein Modifications of O-GlcNAcylation and Phosphorylation Mediate Vernalization Response for Flowering in Winter Wheat
	Xu, Shujuan 2; Xiao, Jun 3; Yin, Fang 2; Guo, Xiaoyu 2; Xing, Lijing ; Xu, Yunyuan ; Chong, Kang 1,2
	2019
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	180 期号:3 页码:1436-1449
摘要	O-GlcNAcylation and phosphorylation are two posttranslational modifications that antagonistically regulate protein function. However, the regulation of and the cross talk between these two protein modifications are poorly understood in plants. Here we investigated the role of O-GlcNAcylation during vernalization, a process whereby prolonged cold exposure promotes flowering in winter wheat (Triticum aestivum), and analyzed the dynamic profile of O-GlcNAcylated and phosphorylated proteins in response to vernalization. Altering O-GlcNAc signaling by chemical inhibitors affected the vernalization response, modifying the expression of VRN genes and subsequently affecting flowering transition. Over a vernalization time-course, O-GlcNAcylated and phosphorylated peptides were enriched from winter wheat plumules by Lectin weak affinity chromatography and iTRAQ-TiO2, respectively. Subsequent mass spectrometry and gene ontology term enrichment analysis identified 168 O-GlcNAcylated proteins that are mainly involved in responses to abiotic stimulus and hormones, metabolic processing, and gene expression; and 124 differentially expressed phosphorylated proteins that participate in translation, transcription, and metabolic processing. Of note, 31 vernalization-associated proteins were identified that carried both phosphorylation and O-GlcNAcylation modifications, of which the majority (97%) exhibited the coexisting module and the remainder exhibited the potential competitive module. Among these, TaGRP2 was decorated with dynamic O-GlcNAcylation (S87) and phosphorylation (S152) modifications, and the mutation of S87 and S152 affected the binding of TaGRP2 to the RIP3 motif of TaVRN1 in vitro. Our data suggest that a dynamic network of O-GlcNAcylation and phosphorylation at key pathway nodes regulate the vernalization response and mediate flowering in wheat.
学科领域	Plant Sciences
DOI	10.1104/pp.19.00081
收录类别	SCI
语种	英语
WOS关键词	GLCNAC TRANSFERASE ; CROSS-TALK ; POSTTRANSLATIONAL MODIFICATION ; FUNCTIONAL-ANALYSIS ; ANALYSIS REVEALS ; GENE ; ROLES ; TRANSCRIPTION ; CLONING ; FLC
WOS研究方向	Plant Sciences
WOS记录号	WOS:000473274000019
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	National Key Research and Development Program of China [2016YFD0101004] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation
作者邮箱	chongk@ibcas.ac.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19834
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Xu, Shujuan,Xiao, Jun,Yin, Fang,et al. The Protein Modifications of O-GlcNAcylation and Phosphorylation Mediate Vernalization Response for Flowering in Winter Wheat[J]. PLANT PHYSIOLOGY,2019,180(3):1436-1449.
APA	Xu, Shujuan.,Xiao, Jun.,Yin, Fang.,Guo, Xiaoyu.,Xing, Lijing.,...&Chong, Kang.(2019).The Protein Modifications of O-GlcNAcylation and Phosphorylation Mediate Vernalization Response for Flowering in Winter Wheat.PLANT PHYSIOLOGY,180(3),1436-1449.
MLA	Xu, Shujuan,et al."The Protein Modifications of O-GlcNAcylation and Phosphorylation Mediate Vernalization Response for Flowering in Winter Wheat".PLANT PHYSIOLOGY 180.3(2019):1436-1449.

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