Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism

doi:10.3390/ijms20092339

	Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism
	Xue, Shan 1; Zou, Junjie 2; Liu, Yangfan 1; Wang, Ming 1,3; Zhang, Chunxia ; Le, Jie
	2019
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	20 期号:9
摘要	ADP-ribosylation factor-guanine nucleotide exchange factors (ARF-GEFs) act as key regulators of vesicle trafficking in all eukaryotes. In Arabidopsis, there are eight ARF-GEFs, including three members of the GBF1 subfamily and five members of the BIG subfamily. These ARF-GEFs have different subcellular localizations and regulate different trafficking pathways. Until now, the roles of these BIG-subfamily ARF-GEFs have not been fully revealed. Here, analysis of the BIGs expression patterns showed that BIG3 and BIG5 have similar expression patterns. big5-1 displayed a dwarf growth and big3-1 big5-1 double mutant showed more severe defects, indicating functional redundancy between BIG3 and BIG5. Moreover, both big5-1 and big3-1 big5-1 exhibited a reduced sensitivity to Brassinosteroid (BR) treatment. Brefeldin A (BFA)-induced BR receptor Brassinosteroid insensitive 1 (BRI1) aggregation was reduced in big5-1 mutant, indicating that the action of BIG5 is required for BRI1 recycling. Furthermore, BR-induced dephosphorylation of transcription factor BZR1 was decreased in big3-1 big5-1 double mutants. The introduction of the gain-of-function of BZR1 mutant BZR1-1D in big3-1 big5-1 mutants can partially rescue the big3-1 big5-1 growth defects. Our findings revealed that BIG5 functions redundantly with BIG3 in plant growth and gravitropism, and BIG5 participates in BR signal transduction pathway through regulating BRI1 trafficking.
关键词	ARF protein BIG family ARF-GEF auxin brassinosteroids gravity response
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms20092339
收录类别	SCI
语种	英语
WOS关键词	BRASSINOSTEROID INSENSITIVE1 ; MEDIATES ENDOCYTOSIS ; AUXIN GRADIENTS ; GENE-EXPRESSION ; CELL-DIVISION ; ARABIDOPSIS ; RECEPTOR ; GOLGI ; KINASE ; GNOM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000469753500286
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2011CB710902] ; Strategic Pioneer Program on Space Science, Chinese Academy of Sciences [XDA04079100] ; National Natural Science Foundation of China programNational Natural Science Foundation of China (NSFC) [30971652, 31271463]
作者邮箱	xueshan1984@yeah.net ; zoujunjie@caas.cn ; liuyangfan@ibcas.ac.cn ; ming.wang@genefirst.com ; chunxia.zhang@ibcas.ac.cn ; lejie@ibcas.ac.cn
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19835
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 4.GeneFirst Ltd, Abingdon OX14 3DB, Oxon, England
推荐引用方式 GB/T 7714	Xue, Shan,Zou, Junjie,Liu, Yangfan,et al. Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(9).
APA	Xue, Shan,Zou, Junjie,Liu, Yangfan,Wang, Ming,Zhang, Chunxia,&Le, Jie.(2019).Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(9).
MLA	Xue, Shan,et al."Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.9(2019).

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