Knowledge Management System Of Institute Of Botany,CAS
Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism | |
Xue, Shan1; Zou, Junjie2; Liu, Yangfan1; Wang, Ming1,3; Zhang, Chunxia; Le, Jie | |
2019 | |
发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
卷号 | 20期号:9 |
摘要 | ADP-ribosylation factor-guanine nucleotide exchange factors (ARF-GEFs) act as key regulators of vesicle trafficking in all eukaryotes. In Arabidopsis, there are eight ARF-GEFs, including three members of the GBF1 subfamily and five members of the BIG subfamily. These ARF-GEFs have different subcellular localizations and regulate different trafficking pathways. Until now, the roles of these BIG-subfamily ARF-GEFs have not been fully revealed. Here, analysis of the BIGs expression patterns showed that BIG3 and BIG5 have similar expression patterns. big5-1 displayed a dwarf growth and big3-1 big5-1 double mutant showed more severe defects, indicating functional redundancy between BIG3 and BIG5. Moreover, both big5-1 and big3-1 big5-1 exhibited a reduced sensitivity to Brassinosteroid (BR) treatment. Brefeldin A (BFA)-induced BR receptor Brassinosteroid insensitive 1 (BRI1) aggregation was reduced in big5-1 mutant, indicating that the action of BIG5 is required for BRI1 recycling. Furthermore, BR-induced dephosphorylation of transcription factor BZR1 was decreased in big3-1 big5-1 double mutants. The introduction of the gain-of-function of BZR1 mutant BZR1-1D in big3-1 big5-1 mutants can partially rescue the big3-1 big5-1 growth defects. Our findings revealed that BIG5 functions redundantly with BIG3 in plant growth and gravitropism, and BIG5 participates in BR signal transduction pathway through regulating BRI1 trafficking. |
关键词 | ARF protein BIG family ARF-GEF auxin brassinosteroids gravity response |
学科领域 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
DOI | 10.3390/ijms20092339 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | BRASSINOSTEROID INSENSITIVE1 ; MEDIATES ENDOCYTOSIS ; AUXIN GRADIENTS ; GENE-EXPRESSION ; CELL-DIVISION ; ARABIDOPSIS ; RECEPTOR ; GOLGI ; KINASE ; GNOM |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS记录号 | WOS:000469753500286 |
出版者 | MDPI |
文献子类 | Article |
出版地 | BASEL |
EISSN | 1422-0067 |
资助机构 | National Basic Research Program of ChinaNational Basic Research Program of China [2011CB710902] ; Strategic Pioneer Program on Space Science, Chinese Academy of Sciences [XDA04079100] ; National Natural Science Foundation of China programNational Natural Science Foundation of China (NSFC) [30971652, 31271463] |
作者邮箱 | xueshan1984@yeah.net ; zoujunjie@caas.cn ; liuyangfan@ibcas.ac.cn ; ming.wang@genefirst.com ; chunxia.zhang@ibcas.ac.cn ; lejie@ibcas.ac.cn |
作品OA属性 | Green Submitted, Green Published, gold |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19835 |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China 4.GeneFirst Ltd, Abingdon OX14 3DB, Oxon, England |
推荐引用方式 GB/T 7714 | Xue, Shan,Zou, Junjie,Liu, Yangfan,et al. Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(9). |
APA | Xue, Shan,Zou, Junjie,Liu, Yangfan,Wang, Ming,Zhang, Chunxia,&Le, Jie.(2019).Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(9). |
MLA | Xue, Shan,et al."Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.9(2019). |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Xue-2019-Involvement(3441KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论