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| OsMTOPVIB is required for meiotic bipolar spindle assembly | |
| Xue, Zhihui1,2; Liu, Changzhen1; Shi, Wenqing1; Miao, Yongjie1; Shen, Yi1; Tang, Ding1; Li, Yafei1; You, Aiqing3; Xu, Yunyuan2; Chong, Kang2; Cheng, Zhukuan1 | |
| 2019 | |
| 发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| ISSN | 0027-8424 |
| 卷号 | 116期号:32页码:15967-15972 |
| 摘要 | The organization of microtubules into a bipolar spindle is essential for chromosome segregation. Both centrosome and chromat-independent spindle assembly mechanisms are well studied in mouse, Drosophila melanogaster, and Xenopus oocytes; however, the mechanism of bipolar spindle assembly in plant meiosis remains elusive. According to our observations of microtubule assembly in Oryza sativa, Zea mays, Arabidopsis thaliana, and Solanum lycopersicum, we propose that a key step of plant bipolar spindle assembly is the correction of the multipolar spindle into a bipolar spindle at metaphase I. The multipolar spindles failed to transition into bipolar ones in OsmtopVIB with the defect in double-strand break (DSB) formation. However, bipolar spindles were normally assembled in several other mutants lacking DSB formation, such as Osspo11-1, pair2, and crc1, indicating that bipolar spindle assembly is independent of DSB formation. We further revealed that the mono-orientation of sister kinetochores was prevalent in OsmtopVIB, whereas biorientation of sister kinetochores was frequently observed in Osspo11-1, pair2, and crc1. In addition, mutations of the cohesion subunit OsREC8 resulted in biorientation of sister kinetochores as well as bipolar spindles even in the background of OsmtopVIB. Therefore, we propose that biorientation of the kinetochore is required for bipolar spindle assembly in the absence of homologous recombination. |
| 关键词 | rice meiosis spindle assembly kinetochore |
| 学科领域 | Multidisciplinary Sciences |
| DOI | 10.1073/pnas.1821315116 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | GAMMA-TUBULIN ; RECOMBINATION INITIATION ; MONOPOLAR ORIENTATION ; CHROMATID COHESION ; SELF-ORGANIZATION ; PLANT-CELLS ; MEIOSIS ; CHROMOSOME ; MICROTUBULES ; DIVISION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS记录号 | WOS:000478971900037 |
| 出版者 | NATL ACAD SCIENCES |
| 文献子类 | Article |
| 出版地 | WASHINGTON |
| 资助机构 | National Key Research and Development Program of China [2016YFD0100901] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31771363] |
| 作者邮箱 | zkcheng@genetics.ac.cn |
| 作品OA属性 | Bronze, Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19836 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.Hubei Acad Agr Sci, Food Crops Inst, Wuhan 430064, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Zhihui,Liu, Changzhen,Shi, Wenqing,et al. OsMTOPVIB is required for meiotic bipolar spindle assembly[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(32):15967-15972. |
| APA | Xue, Zhihui.,Liu, Changzhen.,Shi, Wenqing.,Miao, Yongjie.,Shen, Yi.,...&Cheng, Zhukuan.(2019).OsMTOPVIB is required for meiotic bipolar spindle assembly.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(32),15967-15972. |
| MLA | Xue, Zhihui,et al."OsMTOPVIB is required for meiotic bipolar spindle assembly".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.32(2019):15967-15972. |
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| Xue-2019-OsMTOPVIB i（1262KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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