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| Ectopic expression of ORANGE promotes carotenoid accumulation and fruit development in tomato | |
Yazdani, Mohammad1; Sun, Zhaoxia1,2; Yuan, Hui1; Zeng, Shaohua1,3; Thannhauser, Theodore W.; Vrebalov, Julia; Ma, Qiyue; Xu, Yimin; Fei, Zhangjun; Van Eck, Joyce1; Tian, Shiping5 ; Tadmor, Yaakov6; Giovannoni, James J.; Li, Li1
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| 2019 | |
| 发表期刊 | PLANT BIOTECHNOLOGY JOURNAL
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| ISSN | 1467-7644 |
| 卷号 | 17期号:1页码:33-49 |
| 摘要 | Carotenoids are critically important to plants and humans. The ORANGE (OR) gene is a key regulator for carotenoid accumulation, but its physiological roles in crops remain elusive. In this study, we generated transgenic tomato ectopically overexpressing the Arabidopsis wild-type OR (AtORWT) and a 'golden SNP'-containing OR (AtORHis). We found that AtORHis initiated chromoplast formation in very young fruit and stimulated carotenoid accumulation at all fruit developmental stages, uncoupled from other ripening activities. The elevated levels of carotenoids in the AtOR lines were distributed in the same subplastidial fractions as in wild-type tomato, indicating an adaptive response of plastids to sequester the increased carotenoids. Microscopic analysis revealed that the plastid sizes were increased in both AtORWT and AtORHis lines at early fruit developmental stages. Moreover, AtOR overexpression promoted early flowering, fruit set and seed production. Ethylene production and the expression of ripening-associated genes were also significantly increased in the AtOR transgenic fruit at ripening stages. RNA-Seq transcriptomic profiling highlighted the primary effects of OR overexpression on the genes in the processes related to RNA, protein and signalling in tomato fruit. Taken together, these results expand our understanding of OR in mediating carotenoid accumulation in plants and suggest additional roles of OR in affecting plastid size as well as flower and fruit development, thus making OR a target gene not only for nutritional biofortification of agricultural products but also for alteration of horticultural traits. |
| 关键词 | OR carotenoids plastids early flowering fruit set tomato |
| 学科领域 | Biotechnology & Applied Microbiology ; Plant Sciences |
| DOI | 10.1111/pbi.12945 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | CELL-WALL INVERTASE ; PHYTOENE SYNTHASE ; REGULATORY CONTROL ; CLP PROTEASE ; GENE ; BIOSYNTHESIS ; CHLOROPLAST ; METABOLISM ; MUTANT ; MANIPULATION |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS记录号 | WOS:000455486500005 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1467-7652 |
| 资助机构 | United States-Israel Binational Agricultural Research and Development FundUS-Israel Binational Science Foundation [US-4918-16CR] ; USDA-ARS base fund ; Shanxi Scholarship Council of China [2017-069] ; Division Of Integrative Organismal SystemsNational Science Foundation (NSF)NSF - Directorate for Biological Sciences (BIO) [1339287] Funding Source: National Science Foundation |
| 作者邮箱 | ll37@cornell.edu |
| 作品OA属性 | Green Published, gold |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19848 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14850 USA 2.Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14850 USA 3.Shanxi Agr Univ, Inst Agr Bioengn, Coll Agr, Taigu, Shanxi, Peoples R China 4.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou, Guangdong, Peoples R China 5.Vrebalov, Julia; Ma, Qiyue; Xu, Yimin; Fei, Zhangjun; Van Eck, Joyce; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst, Ithaca, NY USA 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 7.Israeli Agr Res Org, Inst Plant Sci, Newe Yaar Res Ctr, Ramat Yishai, Israel |
| 推荐引用方式 GB/T 7714 | Yazdani, Mohammad,Sun, Zhaoxia,Yuan, Hui,et al. Ectopic expression of ORANGE promotes carotenoid accumulation and fruit development in tomato[J]. PLANT BIOTECHNOLOGY JOURNAL,2019,17(1):33-49. |
| APA | Yazdani, Mohammad.,Sun, Zhaoxia.,Yuan, Hui.,Zeng, Shaohua.,Thannhauser, Theodore W..,...&Li, Li.(2019).Ectopic expression of ORANGE promotes carotenoid accumulation and fruit development in tomato.PLANT BIOTECHNOLOGY JOURNAL,17(1),33-49. |
| MLA | Yazdani, Mohammad,et al."Ectopic expression of ORANGE promotes carotenoid accumulation and fruit development in tomato".PLANT BIOTECHNOLOGY JOURNAL 17.1(2019):33-49. |
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