Knowledge Management System Of Institute Of Botany,CAS
| Unraveling the genetic architecture of grain size in einkorn wheat through linkage and homology mapping and transcriptomic profiling | |
Yu, Kang1,4; Liu, Dongcheng; Chen, Yong5; Wang, Dongzhi2; Yang, Wenlong; Yang, Wei3; Yin, Lixin3; Zhang, Chi3,4; Zhao, Shancen3,4; Sun, Jiazhu; Liu, Chunming1 ; Zhang, Aimin
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| 2019 | |
| 发表期刊 | JOURNAL OF EXPERIMENTAL BOTANY
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| ISSN | 0022-0957 |
| 卷号 | 70期号:18页码:4671-4687 |
| 摘要 | Understanding the genetic architecture of grain size is a prerequisite to manipulating grain development and improving the potential crop yield. In this study, we conducted a whole genome-wide quantitative trait locus (QTL) mapping of grain-size-related traits by constructing a high-density genetic map using 109 recombinant inbred lines of einkorn wheat. We explored the candidate genes underlying QTLs through homologous analysis and RNA sequencing. The high-density genetic map spanned 1873 cM and contained 9937 single nucleotide polymorphism markers assigned to 1551 bins on seven chromosomes. Strong collinearity and high genome coverage of this map were revealed by comparison with physical maps of wheat and barley. Six grain size-related traits were surveyed in five environments. In total, 42 QTLs were identified; these were assigned to 17 genomic regions on six chromosomes and accounted for 52.3-66.7% of the phenotypic variation. Thirty homologous genes involved in grain development were located in 12 regions. RNA sequencing identified 4959 genes differentially expressed between the two parental lines. Twenty differentially expressed genes involved in grain size development and starch biosynthesis were mapped to nine regions that contained 26 QTLs, indicating that the starch biosynthesis pathway plays a vital role in grain development in einkorn wheat. This study provides new insights into the genetic architecture of grain size in einkorn wheat; identification of the underlying genes enables understanding of grain development and wheat genetic improvement. Furthermore, the map facilitates quantitative trait mapping, map-based cloning, genome assembly, and comparative genomics in wheat taxa. |
| 关键词 | Einkorn wheat (Triticum monococcum) grain size high-density genetic map quantitative trait loci RAD-seq RNA-seq |
| 学科领域 | Plant Sciences |
| DOI | 10.1093/jxb/erz247 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | QUANTITATIVE TRAIT LOCUS ; TRITICUM-MONOCOCCUM L. ; HIGH-DENSITY ; BREAD WHEAT ; RNA-SEQ ; MAP ; QTL ; YIELD ; DOMESTICATION ; EVOLUTION |
| WOS研究方向 | Plant Sciences |
| WOS记录号 | WOS:000493107700010 |
| 出版者 | OXFORD UNIV PRESS |
| 文献子类 | Article |
| 出版地 | OXFORD |
| EISSN | 1460-2431 |
| 资助机构 | National Key Research and Development Program of China [2016YFD0101802, 2016YFD0101004] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31571643] |
| 作者邮箱 | cmliu@ibcas.ac.cn ; amzhang@genetics.ac.cn |
| 作品OA属性 | Green Published, hybrid, Green Submitted |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19850 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Innovat Acad Seed Design, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Beijing Genom Inst Shenzhen, Shenzhen 518083, Peoples R China 5.BGI Shenzhen, State Key Lab Agr Genom, Shenzhen 518083, Peoples R China 6.State Tobacco Monopoly Adm, Sci & Technol Dept, Beijing 100045, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Kang,Liu, Dongcheng,Chen, Yong,et al. Unraveling the genetic architecture of grain size in einkorn wheat through linkage and homology mapping and transcriptomic profiling[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(18):4671-4687. |
| APA | Yu, Kang.,Liu, Dongcheng.,Chen, Yong.,Wang, Dongzhi.,Yang, Wenlong.,...&Zhang, Aimin.(2019).Unraveling the genetic architecture of grain size in einkorn wheat through linkage and homology mapping and transcriptomic profiling.JOURNAL OF EXPERIMENTAL BOTANY,70(18),4671-4687. |
| MLA | Yu, Kang,et al."Unraveling the genetic architecture of grain size in einkorn wheat through linkage and homology mapping and transcriptomic profiling".JOURNAL OF EXPERIMENTAL BOTANY 70.18(2019):4671-4687. |
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