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| Modulation of Auxin Signaling and Development by Polyadenylation Machinery | |
Zeng, Wei1; Dai, Xinhua2; Sun, Jing3,4; Hou, Yifeng3,4; Ma, Xuan5; Cao, Xiaofeng1,3,4; Zhao, Yunde2; Cheng, Youfa1
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| 2019 | |
| 发表期刊 | PLANT PHYSIOLOGY
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| ISSN | 0032-0889 |
| 卷号 | 179期号:2页码:686-699 |
| 摘要 | Polyadenylation influences gene expression by affecting mRNA stability, transport, and translatability. Here, we report that Cleavage stimulation Factor 77 (AtCstF77), a component of the pre-mRNA 3'-end polyadenylation machinery, affects polyadenylation site (PAS) selection in transcripts of some auxin signaling genes in Arabidopsis (Arabidopsis thaliana). Disruption of AtCstF77 reduced auxin sensitivity and decreased the expression of the auxin reporter DR5-GFP. Null mutations of cstf77 caused severe developmental defects, but were not lethal as previously reported. cstf77-2 genetically interacted with transport inhibitor response 1 auxin signaling f-box 2 auxin receptor double mutants, further supporting that polyadenylation affects auxin signaling. AtCstF77 was ubiquitously expressed in embryos, seedlings, and adult plants. The AtCstF77 protein was localized in the nucleus, which is consistent with its function in pre-mRNA processing. We observed that PASs in transcripts from approximately 2,400 genes were shifted in the cstf77-2 mutant. Moreover, most of the PAS shifts were from proximal to distal sites. Auxin treatment also caused PAS shifts in transcripts from a small number of genes. Several auxin signaling or homeostasis genes had different PASs in their transcripts in the cstf77-2 mutant. The expression levels of AUXIN RESISTANT 2/INDOLE-3-ACETIC ACID 7 were significantly increased in the cstf77-2 mutant, which can partially account for the auxin resistance phenotype of this mutant. Our results demonstrate that AtCstF77 plays pleiotropic and critical roles in Arabidopsis development. Moreover, disruption of AtCstF64, another component of the polyadenylation machinery, led to developmental defects and reduced auxin response, similar to those of the cstf77-2 mutant. We conclude that AtCstF77 affects auxin responses, likely by controlling PAS selection of transcripts of some auxin signaling components. |
| 学科领域 | Plant Sciences |
| DOI | 10.1104/pp.18.00782 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | RNA-BINDING PROTEIN ; ALTERNATIVE POLYADENYLATION ; PLANT-GROWTH ; ARABIDOPSIS ; ENCODES ; DEGRADATION ; TIR1 ; MECHANISM ; MUTANTS ; CPSF30 |
| WOS研究方向 | Plant Sciences |
| WOS记录号 | WOS:000457486700025 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 文献子类 | Article |
| 出版地 | ROCKVILLE |
| EISSN | 1532-2548 |
| 资助机构 | National Natural Science Fund of ChinaNational Natural Science Foundation of China (NSFC) [31270330, 91217310, 91017008, 31171389] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943400] ; Hundred Talents Program of the Chinese Academy of SciencesChinese Academy of Sciences ; U.S. National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM114660] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31788103, 91540203] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB27030201] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM114660] Funding Source: NIH RePORTER |
| 作者邮箱 | yfcheng@ibcas.ac.cn |
| 作品OA属性 | Bronze, Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19851 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA 4.Chinese Acad Sci, State Key Lab Plant Genom, Ctr Excellence Mol Plant Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 5.Chinese Acad Sci, Natl Ctr Plant Gene Res, Ctr Excellence Mol Plant Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 6.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Wei,Dai, Xinhua,Sun, Jing,et al. Modulation of Auxin Signaling and Development by Polyadenylation Machinery[J]. PLANT PHYSIOLOGY,2019,179(2):686-699. |
| APA | Zeng, Wei.,Dai, Xinhua.,Sun, Jing.,Hou, Yifeng.,Ma, Xuan.,...&Cheng, Youfa.(2019).Modulation of Auxin Signaling and Development by Polyadenylation Machinery.PLANT PHYSIOLOGY,179(2),686-699. |
| MLA | Zeng, Wei,et al."Modulation of Auxin Signaling and Development by Polyadenylation Machinery".PLANT PHYSIOLOGY 179.2(2019):686-699. |
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