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Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua
Zhai, Tongtong; Wang, Yuefeng1; Liu, Changli2; Liu, Zhaoying; Zhao, Min2; Chang, Yuan; Zhang, Rongshu
2019
发表期刊ACTA PHYSIOLOGIAE PLANTARUM
ISSN0137-5881
卷号41期号:4
摘要Artemisia annua L. is the main source of artemisinin, currently the most effective treatment for malaria. However, an affordable and abundant supply of artemisinin remains elusive. Trichoderma is a biocontrol agent that stimulates plant growth and defense responses, and improves soil quality. To date, relatively few studies have focused on improving leaf biomass and artemisinin production in A. annua using Trichoderma. To investigate the role of T. asperellum ACCC30536 in improving the artemisinin yield of A. annua, field-grown A. annua was inoculated with T. asperellum conidia and grown for 60days. The results showed that leaf artemisinin concentration and dry weight were increased significantly after inoculation. The optimal inoculation dose was 200mL of conidia suspension at 1x10(7) colony-forming units (cfu)/mL, the highest artemisinin concentration was 8.83mg/g, and the highest artemisinin production was 70.6g on day 50. The results of qRT-PCR revealed that expression of genes encoding key enzymes for artemisinin biosynthesis, namely HMGR1, FPS, ADS, CYP71AV1, CPR, DBR, DXS1, and DXR1, was generally upregulated during days 20-50 following induction by Trichoderma. In addition, the moisture, pH stability, organic matter content, and availability of nitrogen, phosphorus, and kalium in inoculated soil were significantly improved. Thus, application of T. asperellum ACCC30536 may offer a novel approach for improving artemisinin production by upregulating the expression of key enzymes for artemisinin biosynthesis, increasing leaf yield, and improving soil fertility.
关键词Trichoderma asperellum Artemisia annua Artemisinin Soil fertility Leaf yield
学科领域Plant Sciences
DOI10.1007/s11738-019-2836-7
收录类别SCI
语种英语
WOS关键词AMORPHA-4,11-DIENE SYNTHASE ; PHYTOPHTHORA-MEGAKARYA ; GENE-EXPRESSION ; BIOSYNTHESIS ; CLONING ; AVAILABILITY ; PYRAMIDALIS ; BIOCONTROL ; GROWTH ; ENZYME
WOS研究方向Plant Sciences
WOS记录号WOS:000461290100001
出版者SPRINGER HEIDELBERG
文献子类Article
出版地HEIDELBERG
EISSN1861-1664
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC: 31370642] ; State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) [201202] ; Natural Science Foundation of Heilongjiang Province of ChinaNatural Science Foundation of Heilongjiang Province [C201216]
作者邮箱zrs_nefu@163.com
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/19852
专题中科院光生物学重点实验室
作者单位1.Northeast Forestry Univ, Coll Landscape Architecture, Harbin 150040, Heilongjiang, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
3.Northeast Forestry Univ, Coll Life Sci, 26 Hexing Rd, Harbin 150040, Heilongjiang, Peoples R China
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Zhai, Tongtong,Wang, Yuefeng,Liu, Changli,et al. Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua[J]. ACTA PHYSIOLOGIAE PLANTARUM,2019,41(4).
APA Zhai, Tongtong.,Wang, Yuefeng.,Liu, Changli.,Liu, Zhaoying.,Zhao, Min.,...&Zhang, Rongshu.(2019).Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua.ACTA PHYSIOLOGIAE PLANTARUM,41(4).
MLA Zhai, Tongtong,et al."Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua".ACTA PHYSIOLOGIAE PLANTARUM 41.4(2019).
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