Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants

doi:10.1007/s11103-019-00846-6

	Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants
	Zhang, Langlang 2; Zhao, Tingting ; Sun, Xiaoming 1; Wang, Yi 1; Du, Chang ; Zhu, Zhenfei 2; Gichuki, Duncan Kiragu 2; Wang, Qingfeng ; Li, Shaohua1 ; Xin, Haiping
	2019
发表期刊	PLANT MOLECULAR BIOLOGY
ISSN	0167-4412
卷号	100 期号:1-2 页码:95-110
摘要	Key messageOverexpression of VaWRKY12, whose nuclear translocation increased under low temperature, enhanced the cold tolerance of Arabidopsis and grapevine calli and significantly increased the expression of antioxidant-related genes.AbstractLow temperature causes injuries to buds during winter and to young shoots during early spring, thereby affecting grapevine quality and yield. Understanding the regulatory mechanisms of cold stress responses is essential for the breeding of new grapevine cultivars with excellent cold tolerance. Previous studies indicated that WRKY family genes are induced by low temperature in grapevine, but their function in cold stress responses was not clear. Here, a cold-induced WRKY gene, named VaWRKY12, was cloned from Vitis amurensis, which displays remarkable cold tolerance. An atypical transmembrane (TM) region was found in its C-terminal region. Transient expression assays showed that VaWRKY12 was localized in the nucleus and cytoplasm at normal temperature but only in the nucleus after cold treatment. By contrast, a truncated version of VaWRKY12 without the TM region was found specifically in the nucleus at normal temperature, and its binding activity to tandem W-box elements in yeast was stronger than that of VaWRKY12, indicating that the TM region might affect the location and function of VaWRKY12. Overexpression of VaWRKY12 enhanced the cold tolerance of transformed Arabidopsis and grapevine calli. Transcriptome data revealed that the expression of genes encoding antioxidant enzymes, including peroxidases and glutathione S-transferases, was upregulated after cold treatment in VaWRKY12-overexpressing grapevine calli compared to the control calli. This study identifies candidate target genes as a basis for further studies on the roles of VaWRKY12 in cold stress responses in grapevine.
关键词	VaWRKY12 Nuclear translocation Cold stress Vitis amurensis Transcriptome analysis
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1007/s11103-019-00846-6
收录类别	SCI
语种	英语
WOS关键词	RETICULUM STRESS-RESPONSE ; FLOWERING-LOCUS-T ; DROUGHT TOLERANCE ; GENE-EXPRESSION ; OSMOTIC-STRESS ; DNA-BINDING ; DISEASE RESISTANCE ; FREEZING TOLERANCE ; LEAF SENESCENCE ; SALICYLIC-ACID
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000467940100009
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5028
资助机构	National Key Research and Development Program of China [2018YFD1000300] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471857] ; Youth Innovation Promotion Association of CAS [2015281] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
作者邮箱	zll88927@126.com ; zhaotingyx0207@126.com ; aspire2018@163.com ; wangyi19881107@163.com ; duchang@wbgcas.cn ; zhuzhenfei16@maits.ucas.ac.cn ; gichukiduncann@gmail.com ; qfwang@wbgcas.cn ; shhli@ibcas.ac.cn ; xinhaiping215@hotmail.com
引用统计	被引频次：41[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19864
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Langlang,Zhao, Tingting,Sun, Xiaoming,et al. Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants[J]. PLANT MOLECULAR BIOLOGY,2019,100(1-2):95-110.
APA	Zhang, Langlang.,Zhao, Tingting.,Sun, Xiaoming.,Wang, Yi.,Du, Chang.,...&Xin, Haiping.(2019).Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants.PLANT MOLECULAR BIOLOGY,100(1-2),95-110.
MLA	Zhang, Langlang,et al."Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants".PLANT MOLECULAR BIOLOGY 100.1-2(2019):95-110.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Zhang-2019-Overexpre（10372KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文