IB-CAS  > 中科院北方资源植物重点实验室
Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants
Zhang, Langlang2; Zhao, Tingting; Sun, Xiaoming1; Wang, Yi1; Du, Chang; Zhu, Zhenfei2; Gichuki, Duncan Kiragu2; Wang, Qingfeng; Li, Shaohua1; Xin, Haiping
2019
Source PublicationPLANT MOLECULAR BIOLOGY
ISSN0167-4412
Volume100Issue:1-2Pages:95-110
AbstractKey messageOverexpression of VaWRKY12, whose nuclear translocation increased under low temperature, enhanced the cold tolerance of Arabidopsis and grapevine calli and significantly increased the expression of antioxidant-related genes.AbstractLow temperature causes injuries to buds during winter and to young shoots during early spring, thereby affecting grapevine quality and yield. Understanding the regulatory mechanisms of cold stress responses is essential for the breeding of new grapevine cultivars with excellent cold tolerance. Previous studies indicated that WRKY family genes are induced by low temperature in grapevine, but their function in cold stress responses was not clear. Here, a cold-induced WRKY gene, named VaWRKY12, was cloned from Vitis amurensis, which displays remarkable cold tolerance. An atypical transmembrane (TM) region was found in its C-terminal region. Transient expression assays showed that VaWRKY12 was localized in the nucleus and cytoplasm at normal temperature but only in the nucleus after cold treatment. By contrast, a truncated version of VaWRKY12 without the TM region was found specifically in the nucleus at normal temperature, and its binding activity to tandem W-box elements in yeast was stronger than that of VaWRKY12, indicating that the TM region might affect the location and function of VaWRKY12. Overexpression of VaWRKY12 enhanced the cold tolerance of transformed Arabidopsis and grapevine calli. Transcriptome data revealed that the expression of genes encoding antioxidant enzymes, including peroxidases and glutathione S-transferases, was upregulated after cold treatment in VaWRKY12-overexpressing grapevine calli compared to the control calli. This study identifies candidate target genes as a basis for further studies on the roles of VaWRKY12 in cold stress responses in grapevine.
KeywordVaWRKY12 Nuclear translocation Cold stress Vitis amurensis Transcriptome analysis
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
DOI10.1007/s11103-019-00846-6
Indexed BySCI
Language英语
WOS KeywordRETICULUM STRESS-RESPONSE ; FLOWERING-LOCUS-T ; DROUGHT TOLERANCE ; GENE-EXPRESSION ; OSMOTIC-STRESS ; DNA-BINDING ; DISEASE RESISTANCE ; FREEZING TOLERANCE ; LEAF SENESCENCE ; SALICYLIC-ACID
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000467940100009
PublisherSPRINGER
SubtypeArticle
Publication PlaceDORDRECHT
EISSN1573-5028
Funding OrganizationNational Key Research and Development Program of China [2018YFD1000300] ; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471857] ; Youth Innovation Promotion Association of CAS [2015281] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
Corresponding Author Emailzll88927@126.com ; zhaotingyx0207@126.com ; aspire2018@163.com ; wangyi19881107@163.com ; duchang@wbgcas.cn ; zhuzhenfei16@maits.ucas.ac.cn ; gichukiduncann@gmail.com ; qfwang@wbgcas.cn ; shhli@ibcas.ac.cn ; xinhaiping215@hotmail.com
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ibcas.ac.cn/handle/2S10CLM1/19864
Collection中科院北方资源植物重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Langlang,Zhao, Tingting,Sun, Xiaoming,et al. Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants[J]. PLANT MOLECULAR BIOLOGY,2019,100(1-2):95-110.
APA Zhang, Langlang.,Zhao, Tingting.,Sun, Xiaoming.,Wang, Yi.,Du, Chang.,...&Xin, Haiping.(2019).Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants.PLANT MOLECULAR BIOLOGY,100(1-2),95-110.
MLA Zhang, Langlang,et al."Overexpression of VaWRKY12, a transcription factor from Vitis amurensis with increased nuclear localization under low temperature, enhances cold tolerance of plants".PLANT MOLECULAR BIOLOGY 100.1-2(2019):95-110.
Files in This Item:
File Name/Size DocType Version Access License
Zhang-2019-Overexpre(10372KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Langlang]'s Articles
[Zhao, Tingting]'s Articles
[Sun, Xiaoming]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Langlang]'s Articles
[Zhao, Tingting]'s Articles
[Sun, Xiaoming]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Langlang]'s Articles
[Zhao, Tingting]'s Articles
[Sun, Xiaoming]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Zhang-2019-Overexpression of VaWRKY12, a trans.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.