Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency

doi:10.1186/s13059-019-1799-8

	Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency
	Zhao, Taolan 1,2; Huan, Qing 1,2; Sun, Jing 1; Liu, Chunyan 1; Hou, Xiuli ; Yu, Xiang ; Silverman, Ian M.; Zhang, Yi 6,7; Gregory, Brian D.; Liu, Chun-Ming; Qian, Wenfeng 1,2,3; Cao, Xiaofeng 1,3,8
	2019
发表期刊	GENOME BIOLOGY
ISSN	1474-760X
卷号	20 期号:1
摘要	Background Polyadenylation plays a key role in producing mature mRNAs in eukaryotes. It is widely believed that the poly(A)-binding proteins (PABs) uniformly bind to poly(A)-tailed mRNAs, regulating their stability and translational efficiency. Results We observe that the homozygous triple mutant of broadly expressed Arabidopsis thaliana PABs, AtPAB2, AtPAB4, and AtPAB8, is embryonic lethal. To understand the molecular basis, we characterize the RNA-binding landscape of these PABs. The AtPAB-binding efficiency varies over one order of magnitude among genes. To identify the sequences accounting for the variation, we perform poly(A)-seq that directly sequences the full-length poly(A) tails. More than 10% of poly(A) tails contain at least one guanosine (G); among them, the G-content varies from 0.8 to 28%. These guanosines frequently divide poly(A) tails into interspersed A-tracts and therefore cause the variation in the AtPAB-binding efficiency among genes. Ribo-seq and genome-wide RNA stability assays show that AtPAB-binding efficiency of a gene is positively correlated with translational efficiency rather than mRNA stability. Consistently, genes with stronger AtPAB binding exhibit a greater reduction in translational efficiency when AtPAB is depleted. Conclusions Our study provides a new mechanism that translational efficiency of a gene can be regulated through the G-content-dependent PAB binding, paving the way for a better understanding of poly(A) tail-associated regulation of gene expression.
关键词	Poly(A) tails Poly(A)-binding proteins PAB binding efficiency Poly(A)-tail G-content mRNA stability Translational efficiency Arabidopsis
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/s13059-019-1799-8
收录类别	SCI
语种	英语
WOS关键词	POLY(A)-BINDING PROTEIN ; RNA-BINDING ; INITIATION-FACTOR ; REVEALS ; TAIL ; GENE ; MULTIPLE ; TRANSCRIPTS ; ALIGNMENT ; COMPLEX
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000483772800002
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31788103, 91540203, 31770874, 31900455, 31571308, 31701096] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943400] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDPB0403, XDB27030201, XDA08020303] ; Key Research Program of Frontier Sciences of Chinese Academy of Sciences [QYZDY-SSW-SMC022] ; State Key Laboratory of Plant Genomics
作者邮箱	wfqian@genetics.ac.cn ; xfcao@genetics.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19880
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Genet Network Biol, Beijing 100101, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 6.Yu, Xiang; Silverman, Ian M.; Gregory, Brian D.] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA 7.ABLife Inc, Lab Genome Regulat & Human Hlth, Wuhan 430075, Hubei, Peoples R China 8.ABLife Inc, Ctr Genome Anal, Wuhan 430075, Hubei, Peoples R China 9.Chinese Acad Sci, Inst Genet & Dev Biol, CAS Ctr Excellence Mol Plant Sci, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Taolan,Huan, Qing,Sun, Jing,et al. Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency[J]. GENOME BIOLOGY,2019,20(1).
APA	Zhao, Taolan.,Huan, Qing.,Sun, Jing.,Liu, Chunyan.,Hou, Xiuli.,...&Cao, Xiaofeng.(2019).Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency.GENOME BIOLOGY,20(1).
MLA	Zhao, Taolan,et al."Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency".GENOME BIOLOGY 20.1(2019).

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