A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato

doi:10.1111/mpp.12774

	A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato
	Zheng, Xueao ; Li, Xiaojing 1; Wang, Bingsen ; Cheng, Dong ; Li, Yanping ; Li, Wenhao ; Huang, Mengshu ; Tan, Xiaodan ; Zhao, Guozhen ; Song, Botao 2; Macho, Alberto P.; Chen, Huilan ; Xie, Conghua
	2019
发表期刊	MOLECULAR PLANT PATHOLOGY
ISSN	1464-6722
卷号	20 期号:4 页码:547-561
摘要	Both Solanum tuberosum and Ralstonia solanacearum phylotype IIB originated in South America and share a long-term co-evolutionary history. However, our knowledge of potato bacterial wilt pathogenesis is scarce as a result of the technical difficulties of potato plant manipulation. Thus, we established a multiple screening system (virulence screen of effector mutants in potato, growth inhibition of yeast and transient expression in Nicotiana benthamiana) of core type III effectors (T3Es) of a major potato pathovar of phylotype IIB, to provide more research perspectives and biological tools. Using this system, we identified four effectors contributing to virulence during potato infection, with two exhibiting multiple phenotypes in two other systems, including RipAB. Further study showed that RipAB is an unknown protein with a nuclear localization signal (NLS). Furthermore, we generated a ripAB complementation strain and transgenic ripAB-expressing potato plants, and subsequent virulence assays confirmed that R. solanacearum requires RipAB for full virulence. Compared with wild-type potato, transcriptomic analysis of transgenic ripAB-expressing potato plants showed a significant down-regulation of Ca2+ signalling-related genes in the enriched Plant-Pathogen Interaction (PPI) gene ontology (GO) term. We further verified that, during infection, RipAB is required for the down-regulation of four Ca2+ sensors, Stcml5, Stcml23, Stcml-cast and Stcdpk2, and a Ca2+ transporter, Stcngc1. Further evidence showed that the immune-associated reactive oxygen species (ROS) burst is attenuated in ripAB transgenic potato plants. In conclusion, a systematic screen of conserved R. solanacearum effectors revealed an important role for RipAB, which interferes with Ca2+-dependent gene expression to promote disease development in potato.
关键词	type III effector Ralstonia solanacearum plant immunity potato calcium signalling
学科领域	Plant Sciences
DOI	10.1111/mpp.12774
收录类别	SCI
语种	英语
WOS关键词	PROGRAMMED CELL-DEATH ; DEPENDENT PROTEIN-KINASES ; III EFFECTORS ; SECRETION SYSTEM ; PLANT-PATHOGEN ; PHYTOPHTHORA-INFESTANS ; NICOTIANA-BENTHAMIANA ; BROWN-ROT ; IN-VIVO ; BACTERIAL
WOS研究方向	Plant Sciences
WOS记录号	WOS:000461896600007
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1364-3703
资助机构	Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2013PY080] ; Initial Project Fund from Huazhong Agricultural University [12012] ; Shanghai Center for Plant Stress Biology (Chinese Academy of Sciences) ; Chinese 1000 Talents Program ; Earmarked Fund for Modern Agro-Industry Technology Research System of China [CARS-09-P07]
作者邮箱	Chenhuilan@mail.hzau.edu.cn
作品OA属性	Green Published, gold
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19884
专题	中科院北方资源植物重点实验室
作者单位	1.Huazhong Agr Univ, Key Lab Potato Biol & Biotechnol, Minist Agr & Rural Affairs, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Hubei, Peoples R China 4.Macho, Alberto P.] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Biol Sci, Shanghai 201602, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Xueao,Li, Xiaojing,Wang, Bingsen,et al. A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato[J]. MOLECULAR PLANT PATHOLOGY,2019,20(4):547-561.
APA	Zheng, Xueao.,Li, Xiaojing.,Wang, Bingsen.,Cheng, Dong.,Li, Yanping.,...&Xie, Conghua.(2019).A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato.MOLECULAR PLANT PATHOLOGY,20(4),547-561.
MLA	Zheng, Xueao,et al."A systematic screen of conserved Ralstonia solanacearum effectors reveals the role of RipAB, a nuclear-localized effector that suppresses immune responses in potato".MOLECULAR PLANT PATHOLOGY 20.4(2019):547-561.

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