Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)

doi:10.1371/journal.pone.0218336

	Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)
	Zheng, Guangshun 1,2; Fan, Cunying 1,2; Di, Shaokang ; Wang, Xuemin ; Gao, Liping 3; Dzyubenko, Nikolay 4; Chapurin, Vladimir 4; Pang, Yongzhen
	2019
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	14 期号:7
摘要	Flavonoids are one of the largest secondary metabolite groups, which are widely present in plants. Flavonoids include anthocyanins, proanthocyanidins, flavonols and isoflavones. In particular, proanthocyanidins possess beneficial effects for ruminant animals in preventing lethal pasture bloat. As a major legume forage, alfalfa (Medicago sativa) contains little proanthocyanidins in foliage to combat bloat. In an attempt to improve proanthocyanidin content in alfalfa foliage, we over-expressed two MYB transcription factors (CsMYB5-1 and CsMYB5-2) from tea plant that is rich in proanthocyanidins. We showed that, via targeted metabolite and transcript analyses, the transgenic alfalfa plants accumulated higher levels of flavonoids in stems/leaves than the control, in particular anthocyanins and proanthocyanidins. Over-expression of CsMYB5-1 and CsMYB5-2 induced the expression levels of genes involved in flavonoid pathway, especially anthocyanin/proanthocyanidin-specific pathway genes DFR, ANS and ANR in stems/leaves. Both anthocyanin/proanthocyanidin content and the expression levels of several genes were conversely decreased in flowers of the transgenic lines than in control. Our results indicated that CsMYB5-1 and CsMYB5-2 differently regulate anthocyanins/proanthocyanidins in stems/leaves and flowers. Our study provides a guide for increasing anthocyanin/proanthocyanidin accumulation in foliage of legume forage corps by genetic engineering. These results also suggest that it is feasible to cultivate new varieties for forage production to potentially solve pasture bloat, by introducing transcription factors from typical plants with high proanthocyanidin level.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0218336
收录类别	SCI
语种	英语
WOS关键词	REGULATES PROANTHOCYANIDIN SYNTHESIS ; TRANSCRIPTION FACTOR ; SEED COAT ; BIOSYNTHESIS ; ANTHOCYANIN ; TRUNCATULA ; R2R3-MYB ; DIFFERENTIATION ; TRANSFORMATION ; GRAPEVINE
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000484921200009
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	Major State Basic Research and Development ProgramNational Basic Research Program of China [2014CB138703] ; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305]
作者邮箱	pangyongzhen@caas.cn
作品OA属性	Green Published, gold, Green Submitted
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/19886
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 4.Anhui Agr Univ, Sch Life Sci, Hefei, Anhui, Peoples R China 5.Russian Vavilov Inst Plant Genet Resources, Fed Res Ctr, St Petersburg, Russia
推荐引用方式 GB/T 7714	Zheng, Guangshun,Fan, Cunying,Di, Shaokang,et al. Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)[J]. PLOS ONE,2019,14(7).
APA	Zheng, Guangshun.,Fan, Cunying.,Di, Shaokang.,Wang, Xuemin.,Gao, Liping.,...&Pang, Yongzhen.(2019).Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa).PLOS ONE,14(7).
MLA	Zheng, Guangshun,et al."Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)".PLOS ONE 14.7(2019).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Zheng-2019-Ectopic e（2824KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文