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| Transcriptome Analysis Reveals the Mechanism of Fluoride Treatment Affecting Biochemical Components in Camellia sinensis | |
| Zhu, Jiaojiao; Pan, Junting1; Nong, Shouhua; Ma, Yuanchun; Xing, Anqi; Zhu, Xujun; Wen, Bo; Fang, Wanping; Wang, Yuhua | |
| 2019 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| ISSN | 1422-0067 |
| 卷号 | 20期号:2 |
| 摘要 | Tea (Camellia sinensis (L.) O. Kuntze), one of the main crops in China, is high in various bioactive compounds including flavonoids, catechins, caffeine, theanine, and other amino acids. C. sinensis is also known as an accumulator of fluoride (F), and the bioactive compounds are affected by F, however, the mechanism remains unclear. Here, the effects of F treatment on the accumulation of F and major bioactive compounds and gene expression were investigated, revealing the molecular mechanisms affecting the accumulation of bioactive compounds by F treatment. The results showed that F accumulation in tea leaves gradually increased under exogenous F treatments. Similarly, the flavonoid content also increased in the F treatment. In contrast, the polyphenol content, free amino acids, and the total catechins decreased significantly. Special amino acids, such as sulfur-containing amino acids and proline, had the opposite trend of free amino acids. Caffeine was obviously induced by exogenous F, while the theanine content peaked after two day-treatment. These results suggest that the F accumulation and content of bioactive compounds were dramatically affected by F treatment. Furthermore, differentially expressed genes (DEGs) related to the metabolism of main bioactive compounds and amino acids, especially the pivotal regulatory genes of catechins, caffeine, and theanine biosynthesis pathways, were identified and analyzed using high-throughput Illumina RNA-Seq technology and qRT-PCR. The expression of pivotal regulatory genes is consistent with the changes of the main bioactive compounds in C. sinensis leaves, indicating a complicated molecular mechanism for the above findings. Overall, these data provide a reference for exploring the possible molecular mechanism of the accumulation of major bioactive components such as flavonoid, catechins, caffeine, theanine and other amino acids in tea leaves in response to fluoride treatment. |
| 关键词 | Camellia sinensis fluoride RNA-Seq transcriptome bioactive compounds |
| 学科领域 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| DOI | 10.3390/ijms20020237 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | HUMAN EXPOSURE ; AMINO-ACIDS ; TEA PLANT ; BIOSYNTHESIS ; EXPRESSION ; QUALITY ; L. ; ALUMINUM ; STRESS ; GENES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000459746500005 |
| 出版者 | MDPI |
| 文献子类 | Article |
| 出版地 | BASEL |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770733] ; earmarked fund for the China Agriculture Research System [CARS-19] ; earmarked fund for Jiangsu Agricultural Industry Technology System [JATS[2018]280] |
| 作者邮箱 | 2016104092@njau.edu.cn ; panjunting@ibcas.ac.cn ; 2017804197@njau.edu.cn ; myc@njau.edu.cn ; 2017104087@njau.edu.cn ; zhuxujun@njau.edu.cn ; njauwb@njau.edu.cn ; fangwp@njau.edu.cn ; wangyuhua@njau.edu.cn |
| 作品OA属性 | Green Published, gold, Green Submitted |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/19897 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Jiaojiao,Pan, Junting,Nong, Shouhua,et al. Transcriptome Analysis Reveals the Mechanism of Fluoride Treatment Affecting Biochemical Components in Camellia sinensis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(2). |
| APA | Zhu, Jiaojiao.,Pan, Junting.,Nong, Shouhua.,Ma, Yuanchun.,Xing, Anqi.,...&Wang, Yuhua.(2019).Transcriptome Analysis Reveals the Mechanism of Fluoride Treatment Affecting Biochemical Components in Camellia sinensis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(2). |
| MLA | Zhu, Jiaojiao,et al."Transcriptome Analysis Reveals the Mechanism of Fluoride Treatment Affecting Biochemical Components in Camellia sinensis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.2(2019). |
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| Zhu-2019-Transcripto（1687KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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