Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata

doi:10.7554/eLife.36316

	Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata
	Diao, Min 1,2; Ren, Sulin 1; Wang, Qiannan ; Qian, Lichao 3; Shen, Jiangfeng ; Liu, Yule 3; Huang, Shanjin
	2018
发表期刊	ELIFE
ISSN	2050-084X
卷号	7
摘要	Here, we demonstrate that Arabidopsis thaliana Formin 2 (AtFH2) localizes to plasmodesmata (PD) through its transmembrane domain and is required for normal intercellular trafficking. Although loss-of-function atfh2 mutants have no overt developmental defect, PD's permeability and sensitivity to virus infection are increased in atfh2 plants. Interestingly, AtFH2 functions in a partially redundant manner with its closest homolog AtFH1, which also contains a PD localization signal. Strikingly, targeting of Class I formins to PD was also confirmed in rice, suggesting that the involvement of Class I formins in regulating actin dynamics at PD may be evolutionarily conserved in plants. In vitro biochemical analysis showed that AtFH2 fails to nucleate actin assembly but caps and stabilizes actin filaments. We also demonstrate that the interaction between AtFH2 and actin filaments is crucial for its function in vivo. These data allow us to propose that AtFH2 regulates PD's permeability by anchoring actin filaments to PD.
学科领域	Biology
DOI	10.7554/eLife.36316
收录类别	SCI
语种	英语
WOS关键词	CLASS-II FORMIN ; ARP2/3 COMPLEX ; PROTEIN ; CYTOSKELETON ; TOBACCO ; LOCALIZATION ; ORGANIZATION ; NUCLEATION ; MECHANISM ; MOVEMENT
WOS记录号	WOS:000443934300001
出版者	ELIFE SCIENCES PUBLICATIONS LTD
文献子类	Article
出版地	CAMBRIDGE
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471266, 31671390, 31121065]
作者邮箱	sjhuang@tsinghua.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：41[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20323
专题	中科院植物分子生理学重点实验室
作者单位	1.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, MOE Key Lab Bioinformat, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Diao, Min,Ren, Sulin,Wang, Qiannan,et al. Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata[J]. ELIFE,2018,7.
APA	Diao, Min.,Ren, Sulin.,Wang, Qiannan.,Qian, Lichao.,Shen, Jiangfeng.,...&Huang, Shanjin.(2018).Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata.ELIFE,7.
MLA	Diao, Min,et al."Arabidopsis formin 2 regulates cell-to-cell trafficking by capping and stabilizing actin filaments at plasmodesmata".ELIFE 7(2018).

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