High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae)

doi:10.3389/fpls.2018.00398

	High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae)
	Lu, Jiangjie 1,2; Liu, Yuyang 1; Xu, Jing 3,4; Mei, Ziwei 5; Shi, Yujun 6; Liu, Pengli 1; He, Jianbo 7; Wang, Xiaotong 3,4; Meng, Yijun 1; Feng, Shangguo 1; Shen, Chenjia 1; Wang, Huizhong 1
	2018
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	9
摘要	Plants of the Dendrobium genus are orchids with not only ornamental value but also high medicinal value. To understand the genetic basis of variations in active ingredients of the stem total polysaccharide contents (STPCs) among different Dendrobium species, it is of paramount importance to understand the mechanism of STPC formation and identify genes affecting its process at the whole genome level. Here, we report the first high-density single-nucleotide polymorphism (SNP) integrated genetic map with a good genome coverage of Dendrobium. The specific-locus amplified fragment sequencing (SLAF-seq) technology led to identification of 7,013,400 SNPs from 1,503,626 high-quality SLAF markers from two parents (Dendrobium moniliforme female x Dendrobium officinale (1 and their interspecific F-1 hybrid population. The final genetic map contained 8, 573 SLAF markers, covering 19 linkage groups (LGs). This genetic map spanned a length of 2,737.49 cM, where the average distance between markers is 0.32 cM. In total, 5 quantitative trait loci (QTL) related to STPC were identified, 3 of which have candidate genes within the confidence intervals of these stable QTLs based on the D. officinale genome sequence. This study will build a foundation up for the mapping of other medicinal-related traits and provide an important reference for the molecular breeding of these Chinese herb.
关键词	Dendrobium Genetic linkage map Polysaccharides Quantitative trait locus (QTL) SNP
学科领域	Plant Sciences
DOI	10.3389/fpls.2018.00398
收录类别	SCI
语种	英语
WOS关键词	SNP DISCOVERY ; LINKAGE MAPS ; IDENTIFICATION ; GENOME ; HUOSHANENSE ; ANTIOXIDANT ; OFFICINALE ; PROTEINS ; TRAIT ; SEQ
WOS记录号	WOS:000428384900001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81503185] ; Major Increase Or Decrease Program In The Central Finance Level [2060302] ; Zhejiang Provincial Key Research & Development Project [2017C02011, 2018C02030] ; Sci-Tech Major Program for New Crop Varieties (Chinese Medicinal Materials) Breeding of Zhejiang Province [2016C02058] ; Hangzhou Normal University [2016QDL005]
作者邮箱	lujj@hznu.edu.cn ; whz62@163.com
作品OA属性	Green Published, gold
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/20350
专题	系统与进化植物学国家重点实验室
作者单位	1.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou, Zhejiang, Peoples R China 2.Hangzhou Normal Univ, Zhejiang Prov Key Lab Genet Improvement & Qual Co, Hangzhou, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Ctr Rare Plant Med Res Zhejiang Prov, Wuyi, Peoples R China 5.Zhejiang ShouXianGu Pharmaceut Co Ltd, Wuyi, Peoples R China 6.Zhejiang Chinese Med Univ, Coll Pharmaceut Sci, Hangzhou, Zhejiang, Peoples R China 7.Zhejiang Gongshang Univ, Sch Foreign Languages, Hangzhou, Zhejiang, Peoples R China 8.Nanjing Agr Univ, Soybean Res Inst, Nanjing, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Lu, Jiangjie,Liu, Yuyang,Xu, Jing,et al. High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae)[J]. FRONTIERS IN PLANT SCIENCE,2018,9.
APA	Lu, Jiangjie.,Liu, Yuyang.,Xu, Jing.,Mei, Ziwei.,Shi, Yujun.,...&Wang, Huizhong.(2018).High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae).FRONTIERS IN PLANT SCIENCE,9.
MLA	Lu, Jiangjie,et al."High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae)".FRONTIERS IN PLANT SCIENCE 9(2018).

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